FlyBase Allele Report: Dmel\stnB[UAS.cEa]

General Information

Symbol

Dmel\stnB^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Estes

FlyBase ID

FBal0151403

Feature type

allele

Associated gene

Dmel\stnB

Associated Insertion(s)

Carried in Construct

P{UAS-stnB.E}

Key Links

Transgenic product class

wild_type

(Estes et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Estes et al., 2003)

Mutagen

in vitro construct

(Estes et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-stnB.E}

Cytology

Description

UASt regulatory sequences drive expression of stnB.

(Estes et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

stnB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

stnB^UAS.cEa, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

NOT Suppressor of

Statement

Reference

stnB^UAS.cEa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Other

Statement

Reference

Scer\GAL4^Mhc.PW, stnA¹², stnB¹², stnB^UAS.cEa has lethal phenotype

(Estes et al., 2003)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

stnB^UAS.cEa, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

NOT Suppressor of

Statement

Reference

stnB^UAS.cEa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of stnB^Scer\UAS.cEa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹ stnB¹/Y ("stn^8P1") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 rescues the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^Mhc.PW does not rescue the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹ stnB¹ ("stn^8P1") larvae. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹⁵ stnB¹⁵ ("stn^C") larvae. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 restores the on/off transients in the electroretinograms of stnA¹⁵ stnB¹⁵ ("stn^C") animals. The extra "satellite" bouton phenotype seen at the neuromuscular junction of stnA¹ stnB¹ ("stn^8P1") larvae is rescued by expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155.