FlyBase Allele Report: Dmel\pk[UAS.cGa]

General Information

Symbol

Dmel\pk^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Gubb

FlyBase ID

FBal0101220

Feature type

allele

Associated gene

Dmel\pk

Associated Insertion(s)

Carried in Construct

P{UAS-pk.G}

Also Known As

UAS-pk, UAS-Pk^pk

Key Links

Transgenic product class

cDNA

(Gubb et al., 1999)

wild_type

(Gubb et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gubb et al., 1999)

wild_type

(Gubb et al., 1999)

Mutagen

in vitro construct

(Gubb et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-pk.G}

Cytology

Description

UASt sequences drive the Pk4a3 cDNA.

(Gubb et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Ehaideb et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^ptc-559.1

(Tree et al., 2002)

abnormal cell polarity | cell non-autonomous, with Scer\GAL4^Ubx.PdC

(Tree et al., 2002)

abnormal planar polarity, with Scer\GAL4^C-765

(Gubb et al., 1999)

abnormal planar polarity, with Scer\GAL4^da.G32

(Gubb et al., 1999)

abnormal planar polarity, with Scer\GAL4^ptc-559.1

(Lee and Adler, 2002)

abnormal planar polarity | adult stage, with Scer\GAL4^ptc.PU

(Wang et al., 2017)

abnormal planar polarity | cell autonomous, with Scer\GAL4^ptc-559.1

(Lu et al., 2010)

bang sensitive, with Scer\GAL4^A51

(Ehaideb et al., 2014)

bang sensitive, with Scer\GAL4^elav-C155

(Ehaideb et al., 2014)

visible, with Scer\GAL4^dpp.blk1

(Jenny et al., 2005)

visible | adult stage, with Scer\GAL4^ptc.PU

(Wang et al., 2017)

Phenotype Manifest In

metatarsus, with Scer\GAL4^C-765

(Gubb et al., 1999)

metatarsus, with Scer\GAL4^da.G32

(Gubb et al., 1999)

microtubule | larval stage, with Scer\GAL4^Toll-6-D42

(Ehaideb et al., 2014)

motor neuron | larval stage, with Scer\GAL4^Toll-6-D42

(Ehaideb et al., 2014)

tarsal segment 1 & joint, with Scer\GAL4^C-765

(Gubb et al., 1999)

tarsal segment 1 & joint, with Scer\GAL4^da.G32

(Gubb et al., 1999)

tarsal segment 2, with Scer\GAL4^C-765

(Gubb et al., 1999)

tarsal segment 3, with Scer\GAL4^C-765

(Gubb et al., 1999)

tarsal segment 4, with Scer\GAL4^C-765

(Gubb et al., 1999)

tarsal segment, with Scer\GAL4^C-765

(Gubb et al., 1999)

tarsal segment, with Scer\GAL4^da.G32

(Gubb et al., 1999)

wing & pupa & cell, with Scer\GAL4^en-e16E

(Gubb et al., 1999)

wing blade dorsal compartment, with Scer\GAL4^Bx-MS1096

(Doyle et al., 2008)

wing hair, with Scer\GAL4^Bx-MS1096

(Doyle et al., 2008)

wing hair, with Scer\GAL4^C-765

(Doyle et al., 2008)

wing hair, with Scer\GAL4^da.G32

(Gubb et al., 1999)

wing hair, with Scer\GAL4^dpp.blk1

(Jenny et al., 2005)

wing hair, with Scer\GAL4^ptc-559.1

(Lu et al., 2010)

wing hair, with Scer\GAL4^ptc.PU

(Wang et al., 2017)

wing hair, with Scer\GAL4^Ubx.PdC

(Tree et al., 2002)

wing hair & 1st posterior cell, with Scer\GAL4^ptc-559.1

(Lee and Adler, 2002)

wing hair & cell & pupa, with Scer\GAL4^en-e16E

(Gubb et al., 1999)

Detailed Description

Statement

Reference

Expression of pk^Scer\UAS.cGa under the control of Scer\GAL4^ptc.PU results in wing hair polarity defects in the adult wings.

(Wang et al., 2017)

Expression of pk^Scer\UAS.cGa under the control of Scer\GAL4^A51 or Scer\GAL4^elav-C155 leads to bang sensitivity.

Expression of pk^Scer\UAS.cGa under the control of Scer\GAL4^Toll-6-D42 results in normal polarity of motor neuron microtubules, but a significant increase in plus-end polymerization, as compared with controls.

(Ehaideb et al., 2014)

Expression of pk^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 results in wing hairs pointing towards the midline within the expression domain. Outside of the expression domain wing hair polarity is normal.

(Lu et al., 2010)

Scer\GAL4^C-765-driven overexpression of pk^Scer\UAS.cGa results in variable localised changes in wing hair polarity and occasional cells producing extra hairs. Wing membrane ridge structure shows some deterioration compared to wild-types, but overall ridge orientation is unchanged.

Scer\GAL4^Bx-MS1096-driven overexpression of pk^Scer\UAS.cGa results in disruption of wing hair polarity and ridge formation, but does not produce consistent wing hair or wing membrane ridge reorientation.

(Doyle et al., 2008)

Expression of pk^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point towards from the expression domain.

(Jenny et al., 2005)

In pk^Scer\UAS.cGa; Scer\GAL4^αTub84B.PL adults, planar polarity in the abdomen is reversed in parts of the anterior compartment of each segment, but is normal in the posterior compartments.

(Lawrence et al., 2004)

When expression is driven by Scer\GAL4^ptc-559.1 hairs point towards the midline in the C region of the wing.

(Lee and Adler, 2002)

When pk^Scer\UAS.cGa is expressed as clones in the wing (driven by Scer\GAL4^Ubx.PdC) produces a cell non-autonomous phenotype where cells distal to the clones point toward the clone. When pk^Scer\UAS.cGa is driven by Scer\GAL4^ptc-559.1, the wing hairs in the region between wing vein L3 and L4, distal to the anterior crossvein point towards the midline of the wing, unlike wild-type wings, where the wing hairs point distally.

(Tree et al., 2002)

When pk^Scer\UAS.cGa is driven by Scer\GAL4^en-e16E, hairs fail to migrate from the distal vertex of pupal wing cells of these mutants, unlike wild-type. Over expression of pk^Scer\UAS.cGa when driven by Scer\GAL4^da.G32 or Scer\GAL4^C-765 gives an extreme tarsal duplication phenotype, including a duplicated socket structure in the proximal T1 segment. Defects are also seen in T2, T3 and T4. Also, when driven by Scer\GAL4^da.G32, swirls are seen in the wing hair orientation with different patterns from wing to wing.

(Gubb et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^ptc-559.1/pk^UAS.cGa is a non-enhancer of abnormal planar polarity phenotype of fy²/fy⁶

(Lee and Adler, 2002)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^ptc-559.1/pk^UAS.cGa is a non-enhancer of wing hair phenotype of fy²/fy⁶

(Lee and Adler, 2002)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/pk^UAS.cGa is a suppressor of wing hair & 1st posterior cell phenotype of in¹

(Lee and Adler, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

pk^UAS.cGa/Scer\GAL4^C-765 partially rescues pk³⁰

(Gubb et al., 1999)

Comments

pk^Scer\UAS.cGa driven by Scer\GAL4^C-765 rescues the wing phenotypes of pk³⁰ flies. The triple row bristles are wild-type in appearance and the wing hairs are almost wild-type in appearance.

(Gubb et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

41777

y¹ w^*; P{UAS-pk.G}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pk^Scer\UAS.cGa

pk^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Gubb

(Gubb et al., 1999)

Secondary FlyBase IDs

References (18)

Report Sections

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