FlyBase Allele Report: Dmel\btd[UAS.cSa]

General Information

Symbol

Dmel\btd^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schock

FlyBase ID

FBal0103887

Feature type

allele

Associated gene

Dmel\btd

Associated Insertion(s)

Carried in Construct

P{UAS-btd.S}

Also Known As

UAS-btd

Key Links

Transgenic product class

wild_type

(Schock et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Schock et al., 1999)

Mutagen

in vitro construct

(Schock et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-btd.S}

Cytology

Description

UAS regulatory sequences drive expression of btd.

(Schock et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

btd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | embryonic stage 6, with Scer\GAL4^{GCN4.nanos.bcd3'UTR}

(Eritano et al., 2020)

homeotic | somatic clone, with Scer\GAL4^Tub.PU

(Estella and Mann, 2010)

visible, with Scer\GAL4^sca-537.4

(Schock et al., 1999)

Phenotype Manifest In

antenna, with Scer\GAL4^ey.PH

(Estella et al., 2003)

antenna | ectopic, with Scer\GAL4^ey.PH

(Estella et al., 2003)

cephalic furrow | embryonic stage 6, with Scer\GAL4^{GCN4.nanos.bcd3'UTR}

(Eritano et al., 2020)

epithelial cell | embryonic stage 6, with Scer\GAL4^{GCN4.nanos.bcd3'UTR}

(Eritano et al., 2020)

eye, with Scer\GAL4^ey.PH

(Estella et al., 2003)

haltere, with Scer\GAL4^bi-omb-Gal4

(Estella et al., 2003)

haltere, with Scer\GAL4^Dll-MD743

(Estella et al., 2003)

haltere, with Scer\GAL4^nub-AC-62

(Estella et al., 2003)

haltere | somatic clone, with Scer\GAL4^Act5C.PP

(Estella et al., 2003)

Keilin organ, with Scer\GAL4^btd-MD808

(Franch-Marro et al., 2006)

leg, with Scer\GAL4^ptc.PU

(Córdoba et al., 2016)

leg | distal | ectopic | somatic clone, with Scer\GAL4^Tub.PU

(Estella and Mann, 2010)

leg | ectopic, with Scer\GAL4^bi-omb-Gal4

(Estella et al., 2003)

leg | ectopic, with Scer\GAL4^Dll-MD743

(Estella et al., 2003)

leg | ectopic, with Scer\GAL4^nub-AC-62

(Estella et al., 2003)

leg | ectopic | proximal | somatic clone, with Scer\GAL4^Tub.PU

(Estella and Mann, 2010)

leg | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

(Estella et al., 2003)

leg joint, with Scer\GAL4^ptc.PU

(Córdoba et al., 2016)

wing, with Scer\GAL4^bi-omb-Gal4

(Estella et al., 2003)

wing, with Scer\GAL4^Dll-MD743

(Estella et al., 2003)

wing, with Scer\GAL4^nub-AC-62

(Estella et al., 2003)

wing, with Scer\GAL4^sca-537.4

(Schock et al., 1999)

wing | somatic clone, with Scer\GAL4^Act5C.PP

(Estella et al., 2003)

wing | somatic clone, with Scer\GAL4^Tub.PU

(Estella and Mann, 2010)

wing margin bristle, with Scer\GAL4^sca-537.4

(Schock et al., 1999)

wing margin bristle | ectopic, with Scer\GAL4^sca-537.4

(Schock et al., 1999)

Detailed Description

Statement

Reference

Embryos expressing btd^UAS.cSa under the control of Scer\GAL4^{GCN4.nos.bcd3'UTR} display cephalic furrow initiation events of "type III+", in which three or more cells undergo shortening simultaneously, and an increase in the number of initiating cells per area unit.

(Eritano et al., 2020)

The expression of btd^Scer\UAS.cSa under the control of Scer\GAL4^ptc.PU, in combination with gal80[ts] to induce expression from third instar larval stage onwards, leads to defects in joint formation, as compared to controls.

(Córdoba et al., 2016)

Clones expressing btd^Scer\UAS.cSa, generated under the control of Scer\GAL4^tub.PU using the MARCM system, show ectopic leg tissues in the wing. Both proximal and distal leg structures can be seen.

(Estella and Mann, 2010)

Expression of btd^Scer\UAS.cSa under the control of Scer\GAL4^btd-MD808 causes the Keilin's organs to develop more than the the wild-type number of three sensory hairs.

(Franch-Marro et al., 2006)

When btd^Scer\UAS.cSa is driven by Scer\GAL4^ey.PH, the original eye disappears and is replaced by an ectopic antenna, which often fuses with the normal one. When btd^Scer\UAS.cSa is driven by Scer\GAL4^nub-AC-62, wings and halteres differentiate leg tissue. The leg tissue in these flies is not arranged in segments but it is possible to recognise pattern elements distal markers such as claws are found as well as tarsal and tibia-like bristles. The amount of leg tissue produced in each of these appendages is greater than the corresponding individual legs. When btd^Scer\UAS.cSa is driven by Scer\GAL4^MD743 or Scer\GAL4^bi-omb-Gal4 sets of leg structures are seen in the wing disc derivatives. btd^Scer\UAS.cSa clones (driven by Scer\GAL4^Act5C.PP) in the haltere and wing differentiate leg tissue. These clones normally appear as either vesicles of tissue segregated from the wing and the haltere, or as protruding tissue. They tend to form leg patterns resembling normal ones, which include the formation of various leg segments. Moreover, these clones develop nearly complete leg patterns independently of the wing region where they localise.

(Estella et al., 2003)

Ectopic expression of btd^Scer\UAS.cSa driven by Scer\GAL4^sca-537.4 causes development of mechanosensory stout bristles in ectopic places in the wing.

(Schock et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, btd^UAS.cSa has homeotic | somatic clone phenotype, suppressible | partially by Dll¹⁷

(Estella and Mann, 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, btd^UAS.cSa has wing | somatic clone phenotype, suppressible | partially by Dll¹⁷

(Estella and Mann, 2010)

Scer\GAL4^Tub.PU, btd^UAS.cSa has leg | distal | ectopic | somatic clone phenotype, suppressible by Dll¹⁷

(Estella and Mann, 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, btd^UAS.cSa has leg | proximal | ectopic | somatic clone phenotype, non-suppressible by Dll¹⁷

(Estella and Mann, 2010)

Other

Statement

Reference

Dll^UAS.cGa, Scer\GAL4^btd-MD808, btd^UAS.cSa has Keilin organ | ectopic phenotype

(Franch-Marro et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

A Dll¹⁷ mutant background partially suppresses the ectopic leg tissue phenotype seen when btd^Scer\UAS.cSa clones are generated under the control of Scer\GAL4^tub.PU using the MARCM system. These clones cannot generate distal leg fates, but are able to produce what appears to be proximal leg tissue.

(Estella and Mann, 2010)

Coexpression of btd^Scer\UAS.cSa and Dll^Scer\UAS.cGa, under the control of Scer\GAL4^btd-MD808, cause ectopic formation of Keilin's organs in otherwise normal abdominal segments. These ectopic organs have more than the wild-type number of three sensory hairs.

(Franch-Marro et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Tub.PU/btd^UAS.cSa fails to rescue Df(1)btd-Sp1

(Estella and Mann, 2010)

Scer\GAL4^Dll-em212/btd^UAS.cSa fails to rescue Df(1)btd-Sp1

(Estella and Mann, 2010)

Comments

Expression of btd^Scer\UAS.cSa (using the MARCM system under the control of Scer\GAL4^tub.PU) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae.

Expression of btd^Scer\UAS.cSa fails to rescue the growth defects seen in Df(1)btd-Sp1 mutant leg clones generated in a minute background during embryogenesis using Scer\FLP1^Scer\UAS.cUa under the control of Scer\GAL4^Dll-em212.

(Estella and Mann, 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26881

w^*; wg^Sp-1/CyO; P{UAS-btd.S}M1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

btd^Scer\UAS.cSa

btd^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schock

(Schock et al., 1999)

Secondary FlyBase IDs

References (10)

Report Sections

Open Close