FlyBase Allele Report: Dmel\Src42A[myri]

General Information

Symbol

Dmel\Src42A^myri

Species

D. melanogaster

Name

myristoylation

FlyBase ID

FBal0103954

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

Also Known As

Src42A^miri

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tateno et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: G2D.

(Tateno et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:5,982,422..5,982,422 [+]

Nucleotide change:

G5982422A

Amino acid change:

G2D | Src42A-PA; G2D | Src42A-PC

Reported amino acid change:

G2D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Tateno et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Tateno et al., 2000)

lethal - all die during embryonic stage | partially

(Murray et al., 2006)

some die during embryonic stage | partially

(Murray et al., 2006)

Phenotype Manifest In

cephalopharyngeal skeleton

(Tateno et al., 2000)

embryo | dorsal closure stage

(Murray et al., 2006)

embryonic/first instar larval cuticle

(Murray et al., 2006)

embryonic/larval dorsal tracheal branch | embryonic stage

(Shindo et al., 2008)

embryonic/larval mouth

(Murray et al., 2006)

embryonic/larval tracheal dorsal trunk | embryonic stage

(Shindo et al., 2008)

embryonic leading edge cell & actomyosin

(Murray et al., 2006)

larval dorsal hair

(Murray et al., 2006)

Detailed Description

Statement

Reference

In Src42A^myri mutant embryos the extension of the dorsal branches lags behind the normally concurrent process of dorsal closure. The dorsal branches also contain fewer stalk cells. The dorsal trunk is a zigzag shape.

(Shindo et al., 2008)

Src42A^myri embryos exhibit defects in leading edge cells: the actomyosin cable is disrupted and dorsal closure is slightly defective. 8% of stage 16 embryos show a very small dorsal hole and the remainder show an irregular arrangement of dorsal hairs. Embryonic lethality is 63% with 60% of unhatched embryos showing malformed mouthparts and a small anterior hole.

(Murray et al., 2006)

50.0 +/- 3.3% of homozygotes die as embryos and most of the remainder die during the first larval instar stage. Homozygous embryos do not show a dorsal open phenotype but have malformed mouth parts.

(Tateno et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Src42A^myri has lethal | embryonic stage phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has lethal | embryonic stage | recessive phenotype, enhanceable by Btk^k05610/Btk^k00206

(Tateno et al., 2000)

Enhancer of

Statement

Reference

Src42A^myri is an enhancer of lethal | embryonic stage phenotype of Btk^k05610/Btk^k00206

(Tateno et al., 2000)

Suppressor of

Statement

Reference

Src42A^myri is a suppressor | partially of increased mortality during development phenotype of CycE^{UAS.Tag:HA.Medsh}, Med^{RNAi.UAS.CycE-HA}, Ras85D^{G12D.UAS.p53sh}, Scer\GAL4^Ser.PU, p53^{RNAi.UAS.RasG12D}

(Sekiya et al., 2023)

Phenotype Manifest In

Enhanced by

Statement

Reference

Src42A^myri has embryonic/larval dorsal tracheal branch | embryonic stage phenotype, enhanceable by Src64B^PI

(Shindo et al., 2008)

Src42A^myri has embryonic leading edge cell & actomyosin phenotype, enhanceable by Df(3L)FER-Δ1

(Murray et al., 2006)

Src42A^myri has embryonic/first instar larval cuticle phenotype, enhanceable by Df(3L)FER-Δ1

(Murray et al., 2006)

Src42A^myri has embryonic leading edge cell & actomyosin phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has embryo | dorsal closure stage phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has embryonic/larval mouth phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has larval dorsal hair phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has embryonic/first instar larval cuticle phenotype, enhanceable by FER^Δex1

(Murray et al., 2006)

Src42A^myri has embryo | dorsal closure stage phenotype, enhanceable by Df(3L)FER-Δ1

(Murray et al., 2006)

Suppressed by

Statement

Reference

Btk^k05610/Btk^k00206, Src42A^myri has embryonic/first instar larval cuticle | dorsal phenotype, suppressible | partially by Jra^Asp.hs.sev

(Tateno et al., 2000)

Other

Statement

Reference

Src42A^myri, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, Src64B^PI has embryonic/first instar larval cuticle | dorsal phenotype

(Tateno et al., 2000)

Btk^k05610/Btk^k00206, Src42A^myri has embryonic/first instar larval cuticle | dorsal phenotype

(Tateno et al., 2000)

Btk^k05610/Btk^k00206, Src42A^myri has embryo | dorsal closure stage phenotype

(Tateno et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 90% of Src42A^myri; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

(Tikhmyanova et al., 2010)

Src64B^PI enhances the tracheal defects seen in Src42A^myri mutant embryos. Many dorsal branches fail to extend correctly, remaining in a multicellular state despite the progression of dorsal closure.

(Shindo et al., 2008)

Src42A^myri; Fps85D^Δex1 double mutant embryos show an enhanced embryonic phenotype compared to Src42A^myri embryos. Src42A^myri; Fps85D^Δex1 leading edge cells show a greater disruption of the actomyosin cable, resulting in a more irregular profile. The embryos show delayed dorsal closure, as 85% are still undergoing closure at late stage 16. Embryonic lethality is increased to 100% (vs 63% in Src42A^myri embryos). 95% of the double mutant embryos show breaks and irregularities in the dorsal hair pattern and a small anterior hole in the mouthparts. The remaining embryos fail to complete dorsal closure and have a large anterior hole.

Src42A^myri; Df(3L)Fps85D-Δ1 embryos show an enhancement of the embryonic phenotypes of both Src42A^myri and Df(3L)Fps85D-Δ1 single mutants. The leading edge cells of Src42A^myri; Df(3L)Fps85D-Δ1 embryos are highly irregular and show a complete loss of the F-actin cable. Cuticle preparations from these embryos show that 30% have a large anterior hole, 59% show a small anterior hole with small scabs along the dorsal midline and the remaining 11% fail to secrete a cuticle.

(Murray et al., 2006)

Btk29A^k00206 Src42A^myri/Btk29A^k05610 Src42A^myri double homozygotes show complete embryonic lethality and some embryos have a dorsal open phenotype. The leading edge cells are only partially elongated during dorsal closure. The dorsal open phenotype is partially rescued by Jra^Asp.hs.sev. Src42A^myri; Src64B^PI double homozygous embryos have a mild but clear dorsal open phenotype.

(Tateno et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6453

w¹¹¹⁸; Src42A^myri/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Src42A^miri

(Tikhmyanova et al., 2010, Shindo et al., 2008)

Src42A^myr

(Shindo et al., 2008)

Src42A^myri

(Sekiya et al., 2023, Shindo et al., 2008, Murray et al., 2006)

Name Synonyms

myristoylation

(Tateno et al., 2000)

Secondary FlyBase IDs

References (5)

Report Sections

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