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General Information
Symbol
Dmel\DUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Mukherjee
FlyBase ID
FBal0104336
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 1.9kb D cDNA clone containing the entire open reading frame is driven by UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of DScer\UAS.cMa under the control of Scer\GAL4GMR.PF results in a moderate eye roughening, with a disorganisation of ommatidia and loss of most mechanosensory bristles. There is a slight loss of pigmentation.

When DScer\UAS.cMa is driven by Scer\GAL4dpp.blk1 at 25oC, animals survive to the pharate adult stage but fail to eclose. These flies exhibit multiple morphological disruptions of eyes, antennae, wings legs and bristles. The arista region of the antenna is generally lacking and replaced by a thick cuticular structure. Some animals exhibit arista-to-tarsal transformation. More proximal regions of the antennae are normal. Legs are severely truncated, as tarsal segments 2-4 are generally fused or missing. In addition both tarsal and tibial regions often exhibit ventralisation, and in some animals, a small distal leg segment is also detected. Eye imaginal discs are greatly reduced and lack a morphogenetic furrow. Flies exhibit moderate to severe loss of eye tissues, with many individuals exhibiting a complete absence of ommatidia. When DScer\UAS.cMa is driven by Scer\GAL430A, flies are viable, but exhibit reduced aristae that are compressed along the proximal/distal axis. The main aristal branch is severely shortened and the side branches are also stunted, though not reduced in number. When driven by Scer\GAL4GMR.PF, flies exhibit a reduction and disorganisation of ommatidia and mechanosensory bristles. At higher doses, a total absence of ommatidia and mechanosensory bristles is seen, replaced by necrotic cell masses. When driven by Scer\GAL4Dll-md23, complete prepupal lethality is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Flies expressing both DScer\UAS.cMa and simScer\UAS.cXa under the control of Scer\GAL4GMR.PF show a stronger disorganisation of ommatidia and mechanosensory bristles in the eye than seen in flies expressing either DScer\UAS.cMa or simScer\UAS.cXa alone under the control of Scer\GAL4GMR.PF. There is also a dramatic loss of eye pigmentation in flies expressing both DScer\UAS.cMa and simScer\UAS.cXa under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DScer\UAS.cMa
DUAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Mukherjee
Secondary FlyBase IDs
    References (2)