FlyBase Allele Report: Dmel\D[UAS.cMa]

General Information

Symbol

Dmel\D^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Mukherjee

FlyBase ID

FBal0104336

Feature type

allele

Associated gene

Dmel\D

Associated Insertion(s)

Carried in Construct

P{UAS-D.M}

Key Links

Transgenic product class

cDNA

(Mukherjee et al., 2000)

wild_type

(Mukherjee et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Mukherjee et al., 2000)

wild_type

(Mukherjee et al., 2000)

Mutagen

in vitro construct

(Mukherjee et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-D.M}

Cytology

Description

A 1.9kb D cDNA clone containing the entire open reading frame is driven by UASt sequences.

(Mukherjee et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PF

(Ma et al., 2000)

viable, with Scer\GAL4^30A

(Mukherjee et al., 2000)

viable, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

viable, with Scer\GAL4^GMR.PF

(Mukherjee et al., 2000)

visible, with Scer\GAL4^30A

(Mukherjee et al., 2000)

visible, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

visible, with Scer\GAL4^GMR.PF

(Ma et al., 2000, Mukherjee et al., 2000)

Phenotype Manifest In

antenna, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

arista, with Scer\GAL4^30A

(Mukherjee et al., 2000)

arista, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

eye, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Ma et al., 2000, Mukherjee et al., 2000)

eye-antennal disc, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Ma et al., 2000, Mukherjee et al., 2000)

leg, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

leg | distal, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

macrochaeta, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

ommatidium, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

ommatidium, with Scer\GAL4^GMR.PF

(Ma et al., 2000, Mukherjee et al., 2000)

pigment cell, with Scer\GAL4^GMR.PF

(Ma et al., 2000)

tarsal segment 2, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

tarsal segment 3, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

tarsal segment 4, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

tarsal segment, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

tarsal segment | ectopic, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

tibia, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

wing, with Scer\GAL4^dpp.blk1

(Mukherjee et al., 2000)

Detailed Description

Statement

Reference

Expression of D^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF results in a moderate eye roughening, with a disorganisation of ommatidia and loss of most mechanosensory bristles. There is a slight loss of pigmentation.

(Ma et al., 2000)

When D^Scer\UAS.cMa is driven by Scer\GAL4^dpp.blk1 at 25^oC, animals survive to the pharate adult stage but fail to eclose. These flies exhibit multiple morphological disruptions of eyes, antennae, wings legs and bristles. The arista region of the antenna is generally lacking and replaced by a thick cuticular structure. Some animals exhibit arista-to-tarsal transformation. More proximal regions of the antennae are normal. Legs are severely truncated, as tarsal segments 2-4 are generally fused or missing. In addition both tarsal and tibial regions often exhibit ventralisation, and in some animals, a small distal leg segment is also detected. Eye imaginal discs are greatly reduced and lack a morphogenetic furrow. Flies exhibit moderate to severe loss of eye tissues, with many individuals exhibiting a complete absence of ommatidia. When D^Scer\UAS.cMa is driven by Scer\GAL4^30A, flies are viable, but exhibit reduced aristae that are compressed along the proximal/distal axis. The main aristal branch is severely shortened and the side branches are also stunted, though not reduced in number. When driven by Scer\GAL4^GMR.PF, flies exhibit a reduction and disorganisation of ommatidia and mechanosensory bristles. At higher doses, a total absence of ommatidia and mechanosensory bristles is seen, replaced by necrotic cell masses. When driven by Scer\GAL4^Dll-md23, complete prepupal lethality is seen.

(Mukherjee et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

D^UAS.cMa, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

D^UAS.cMa, Scer\GAL4^GMR.PF has abnormal eye color phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

D^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

D^UAS.cMa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

D^UAS.cMa, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

D^UAS.cMa, Scer\GAL4^GMR.PF has pigment cell phenotype, enhanceable by sim^UAS.cXa, Scer\GAL4^GMR.PF

(Ma et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing both D^Scer\UAS.cMa and sim^Scer\UAS.cXa under the control of Scer\GAL4^GMR.PF show a stronger disorganisation of ommatidia and mechanosensory bristles in the eye than seen in flies expressing either D^Scer\UAS.cMa or sim^Scer\UAS.cXa alone under the control of Scer\GAL4^GMR.PF. There is also a dramatic loss of eye pigmentation in flies expressing both D^Scer\UAS.cMa and sim^Scer\UAS.cXa under the control of Scer\GAL4^GMR.PF.

(Ma et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

D^Scer\UAS.cMa

D^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Mukherjee

(Mukherjee et al., 2000)

Secondary FlyBase IDs

References (2)

Report Sections

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