FlyBase Allele Report: Dmel\nau[188]

General Information

Symbol

Dmel\nau¹⁸⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0104475

Feature type

allele

Associated gene

Dmel\nau

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wei et al., 2007)

loss of function allele

(Balagopalan et al., 2000)

Mutagen

ethyl methanesulfonate

(Balagopalan et al., 2001)

Progenitor genotype

Cytology

Description

Deletion of sequence from -877 to +1266bp relative to the nau transcription start site.

(Balagopalan et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:23,717,110..23,719,221 [-]

Comment:

Deletion reported as extending from -877 to +1266 relative to the nau transcription start site. This corresponds to a region of 2112bp on the reference sequence. The deletion coordinates were determined from junction sequences in Figure 2.

(Balagopalan et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with Df(3R)nau-11a4)

(Balagopalan et al., 2001)

lethal | recessive

(Wei et al., 2007)

sterile | recessive

(Balagopalan et al., 2001)

viable

(Balagopalan et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

nau¹⁸⁸ embryos exhibit randomisation of the DA3 attachment sites between the wild type DA3 and DO5 positions, as well as the formation of unstructured fibres not resembling any particular muscle. Even when correctly orientated, mutant DA3 fibres are generally thinner than wild type, despite a normal or slightly reduced number of nuclei per cell. In addition, there are many cases of either loss or mis-formation of the DA3, DO4 and DO3 muscles, while other muscles are hardly affected. The severity of these phenotypes vary from embryo to embryo and between segments within the same embryo. There are a small number of DA3>DA2 transformations.

(Enriquez et al., 2012)

In homozygous nau¹⁸⁸ mutant embryos the DA3 muscle is completely absent in around 5% of segments, abnormal in orientation in 45% and normal looking in about 50% of segments. The DA3 fibres in the normal looking nau¹⁸⁸ muscles contain only seven nuclei on average compared to approximately 9 in wild type.

(Dubois et al., 2007)

Homozygous embryos show a range of muscle defects.

Approximately 50% of homozygous embryos do not survive to the pupal stage

(Wei et al., 2007)

Homozygous embryos have a somatic muscle phenotype, in which only a small subset of somatic muscles is affected, including muscles DO4 and DA3. 39% of homozygous eggs derived from homozygous female germline clones (lacking both maternal and zygotic nau function) survive to adulthood. The remaining 61% of eggs appear to be unfertilised. Homozygous embryos derived from homozygous female germline clones show somatic muscle defects identical to those seen for nau¹⁸⁸ zygotic mutants, with the most severely affected muscles including DO4 and DA3.

(Balagopalan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

nau¹⁸⁸ has muscle cell of A1-7 dorsal acute muscle 3 phenotype, enhanceable by kn[+]/kn^col85-GAL4

(Enriquez et al., 2012)

nau¹⁸⁸ has muscle cell of A1-7 dorsal acute muscle 3 phenotype, enhanceable by kn^col85-GAL4/kn^col85-GAL4

(Enriquez et al., 2012)

Suppressed by

Statement

Reference

kn[+]/kn^col85-GAL4, nau¹⁸⁸ has muscle cell of A1-7 dorsal acute muscle 3 phenotype, suppressible by kn^UAS.Tag:HA/Scer\GAL4^{kn-col85-GAL4}

(Enriquez et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

kn^col85-GAL4/+ and, more so, kn^col85-GAL4/kn^col85-GAL4 aggravates the DA3 muscle defects seen in nau¹⁸⁸ embryos. Inclusion of kn^col85-GAL4 also increases the number of ill-formed muscles at the expense of DA3>DA2 muscle transformations. However, the fraction of DA3>DO5 transformations and DA3 +DO5 bifid fibres is not significantly increased by lowering kn levels.

Scer\GAL4^{kn-col85-GAL4}-mediated expression of kn^{Scer\UAS.T:Ivir\HA1} fully rescues the DA3>DA2 phenotype and decreased the number of ill-formed muscles of nau¹⁸⁸, kn^col85-GAL4/+ animals. The fraction of DA3>DO5 transformations and bifid fibres is unaffected.

(Enriquez et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92177

ru¹ st¹ e¹ nau¹⁸⁸/TM3, P{HZR+6.8Xb}JG1, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nau¹⁸⁸

(Choi et al., 2008, Dubois et al., 2007, Wei et al., 2007)

nau^CK188

(Enriquez et al., 2012)

nau^ck188

(Enriquez et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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