FlyBase Allele Report: Dmel\kn[col85-GAL4]

General Information

Symbol

Dmel\kn^col85-GAL4

Species

D. melanogaster

Name

FlyBase ID

FBal0267751

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

P{GAL4}kn^col85-GAL4

Carried in Construct

Also Known As

Pcol85-Gal4, col-Gal4, collier-Gal4, PCol85Gal4, pcol-Gal4

Key Links

Allele class

hypomorphic allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele

(Enriquez et al., 2012)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GAL4}kn^col85-GAL4

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GAL4}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

muscle cell of A1-7 dorsal acute muscle 2 | ectopic

(Enriquez et al., 2012)

muscle cell of A1-7 dorsal acute muscle 2 | ectopic (with kn^col-1)

(Enriquez et al., 2012)

muscle cell of A1-7 dorsal acute muscle 3

(Enriquez et al., 2012)

muscle cell of A1-7 dorsal acute muscle 3 (with kn^col-1)

(Enriquez et al., 2012)

Detailed Description

Statement

Reference

Heterozygous kn^col85-GAL4 embryos have a few 'angled' muscles maintaining both dorsal and ventral anterior attachment sites, indicating a partial DA3>DA2 transformation. The penetrance of this phenotype increases in homozygotes and, in addition, 7% of segments show a complete DA3>DA2 morphological transformation.

40% of segments in kn^col85-GAL4/kn^col-1 embryos show a complete DA3>DA2 morphological transformation. In most other segments, the DA3>DA2 muscle maintains thin projections toward the wild type DA3 attachment site, suggesting an incomplete transformation.

(Enriquez et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

kn[+]/kn^col85-GAL4, nau¹⁸⁸ has muscle cell of A1-7 dorsal acute muscle 3 phenotype, suppressible by kn^UAS.Tag:HA/Scer\GAL4^{kn-col85-GAL4}

(Enriquez et al., 2012)

Enhancer of

Statement

Reference

kn[+]/kn^col85-GAL4 is an enhancer of muscle cell of A1-7 dorsal acute muscle 3 phenotype of nau¹⁸⁸

(Enriquez et al., 2012)

kn^col85-GAL4/kn^col85-GAL4 is an enhancer of muscle cell of A1-7 dorsal acute muscle 3 phenotype of nau¹⁸⁸

(Enriquez et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

kn^col85-GAL4/+ and, more so, kn^col85-GAL4/kn^col85-GAL4 aggravates the DA3 muscle defects seen in nau¹⁸⁸ embryos. Inclusion of kn^col85-GAL4 also increases the number of ill-formed muscles at the expense of DA3>DA2 muscle transformations. However, the fraction of DA3>DO5 transformations and DA3 +DO5 bifid fibres is not significantly increased by lowering kn levels.

(Enriquez et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

kn^col85-GAL4

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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