FlyBase Allele Report: Dmel\Su(H)[VP16.UAS]

General Information

Symbol

Dmel\Su(H)^VP16.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0117640

Feature type

allele

Associated gene

Dmel\Su(H)

Associated Insertion(s)

Carried in Construct

P{UAS-Su(H).VP16.C}

Also Known As

UAS-Su(H)VP16, UAS-Su(H)-vp16, UAS-Su(H)^VP16

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Cooper et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Su(H).VP16.C}

Cytology

Description

UASt regulatory sequences drive expression of amino acids 18 to 594 of Su(H) fused in frame to the VP16 transcriptional activation domain.

(Cooper et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Su(H)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^pnt-14-94

(Liu et al., 2017)

increased cell number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

increased cell number | third instar larval stage, with Scer\GAL4^pnt-14-94

(Liu et al., 2017)

increased occurrence of cell division | larval stage

(Baonza and Freeman, 2005)

Phenotype Manifest In

embryonic hindgut, with Scer\GAL4^455.2

(Iwaki and Lengyel, 2002)

embryonic hindgut, with Scer\GAL4^byn-Gal4

(Iwaki and Lengyel, 2002, Takashima et al., 2002)

embryonic large intestine, with Scer\GAL4^byn-Gal4

(Takashima et al., 2002)

eye disc, with Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

larval brain | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

larval brain | third instar larval stage, with Scer\GAL4^pnt-14-94

(Liu et al., 2017)

muscle cell of A1-7 dorsal acute muscle 1, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

muscle cell of mesothoracic dorsal acute muscle 1, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

muscle cell of metathoracic dorsal acute muscle 1, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

neuroblast | third instar larval stage, with Scer\GAL4^pnt-14-94

(Liu et al., 2017)

pericardial cell, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

pericardial cell | ectopic, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

wing, with Scer\GAL4^dpp.blk1

(Furriols and Bray, 2000)

wing, with Scer\GAL4^ptc-559.1

(Furriols and Bray, 2000)

wing margin | ectopic, with Scer\GAL4^dpp.blk1

(Furriols and Bray, 2000)

wing margin | ectopic, with Scer\GAL4^ptc-559.1

(Furriols and Bray, 2000)

wing vein L3, with Scer\GAL4^dpp.blk1

(Furriols and Bray, 2000)

Detailed Description

Statement

Reference

The expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of either Scer\GAL4^pnt-14-94 or Scer\GAL4^insc-Mz1407 leads to a severe increase in the number of neuroblasts per third instar larval brain lobe, as compared to controls.

(Liu et al., 2017)

Expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^GMR.PF results in excess mitoses in the eye disc posterior to the morphogenetic furrow. There is also a smaller increase in mitotic figures anterior to the morphogenetic furrow.

(Baonza and Freeman, 2005)

When Su(H)^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^eve.eme, in some segments no eve expressing cells form and in others more eve expressing pericardial cells and no DA1 muscles form.

(Han and Bodmer, 2003)

Expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^byn-Gal4 results in embryos with hindguts that are dramatically shorter and wider than wild type. Expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^455.2 does not affect hindgut elongation, but ectopic hindgut boundary cells are seen. A large portion of the hindgut shows a reversal of left-right looping.

(Iwaki and Lengyel, 2002)

Expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^byn-Gal4 in the embryo results in the entire large intestine differentiating into boundary cells.

(Takashima et al., 2002)

Wings from flies expressing Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^ptc-559.1 are distorted by extensive outgrowth that is accompanied by ectopic wing margin structures. When Su(H)^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^dpp.blk1 an ectopic wing margin is formed, the proximal part of the wing is altered, and the L3 vein is wider.

(Furriols and Bray, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Su(H)^VP16.UAS has increased occurrence of cell division | larval stage phenotype, non-enhanceable by Scer\GAL4^GMR.PF/Delta^UAS.cJa

(Baonza and Freeman, 2005)

Suppressed by

Statement

Reference

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has increased cell number | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^pnt-14-94

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has increased cell number | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^pnt-14-94

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Su(H)^VP16.UAS has eye disc phenotype, non-enhanceable by Scer\GAL4^GMR.PF/Delta^UAS.cJa

(Baonza and Freeman, 2005)

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^pnt-14-94/Cdk9^D199N.UAS.SR

(Liu et al., 2017)

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Cdk9^D199N.UAS.SR

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has larval brain | third instar larval stage phenotype, suppressible by Su(Tpl)^HMS00277/Scer\GAL4^pnt-14-94

(Liu et al., 2017)

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^pnt-14-94/Cdk9^D199N.UAS.SR

(Liu et al., 2017)

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Cdk9^D199N.UAS.SR

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS has neuroblast | third instar larval stage phenotype, suppressible by Su(Tpl)^HMS00277/Scer\GAL4^pnt-14-94

(Liu et al., 2017)

Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS has neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Su(H)^VP16.UAS has eye disc phenotype, non-suppressible by Scer\GAL4^GMR.PF/Delta^UAS.cJa

(Baonza and Freeman, 2005)

NOT Suppressor of

Statement

Reference

Su(H)^VP16.UAS/Scer\GAL4^GMR.PF is a non-suppressor of eye disc | somatic clone phenotype of E2f1^rM729

(Baonza and Freeman, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of neuroblasts in third instar larval brain lobes resulting from the expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of either Scer\GAL4^pnt-14-94 or Scer\GAL4^insc-Mz1407 is fully suppressed by the co-expression of either Cdk9^{D199N.Scer\UAS.SR} or Su(Tpl)^HMS00277.

(Liu et al., 2017)

Scer\GAL4^GMR.PF-driven co-expression of Su(H)^{Scer\UAS.T:Hsim\VP16} and Dl^Scer\UAS.cJa results in an expansion of the zone of BrdU incorporation (a marker of S phases) and an increase in the number of cell divisions. Co-expression of Su(H)^{Scer\UAS.T:Hsim\VP16} and Dl^Scer\UAS.cJa gives the same degree of excess proliferation in the eye disc as expression of Su(H)^{Scer\UAS.T:Hsim\VP16} alone.

Scer\GAL4^GMR.PF-driven expression of Su(H)^{Scer\UAS.T:Hsim\VP16} is unable to rescue the small size of E2f^rM729 eye disc clones.

(Baonza and Freeman, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Su(H)^{Scer\UAS.T:Hsim\VP16}

Su(H)^VP16.UAS

Name Synonyms

Secondary FlyBase IDs

References (14)

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