FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pak20
Open Close
General Information
Symbol
Dmel\Pak20
Species
D. melanogaster
Name
FlyBase ID
FBal0117677
Feature type
allele
Associated gene
Dmel\Pak
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Amino acid replacement: Q281term.

    (Newsome et al., 2000)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    3R:6,357,109..6,357,109 [+]
    Nucleotide change:

    C6357109T

    Amino acid change:

    Q281term | Pak-PA; Q281term | Pak-PB; Q281term | Pak-PC; Q281term | Pak-PE; Q281term | Pak-PF; Q281term | Pak-PG; Q281term | Pak-PH; Q281term | Pak-PI; Q281term | Pak-PJ

    Reported amino acid change:

    Q281term

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    (Newsome et al., 2000)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Embryos lacking both maternal and zygotic Pak[+] function (derived from females containing homozygous germline clones mated to heterozygous males) show guidance defects in the VO5, VO6 and VA3 muscles; affected muscles (50%) bypass their normal ventral attachment sites and mistarget towards or cross the ventral midline. In addition, an increase in LT and VA3 muscle numbers is seen and the LO1 and VO1 muscles ae defective in shape.

        (Bahri et al., 2009)

        The cuticle of embryos lacking both maternal and zygotic Pak show dorsal pucker, 'scab' and head defects. About 41% of embryos exhibit dorsal closure defects. The leading edge cytoskeleton is considerably depleted, though the dorsal-most epidermal cells show considerable elongation in the dorsal-ventral direction.

        (Conder et al., 2004)

        Defects in pathfinding by photoreceptor axons are seen in mosaic animals in which virtually the entire retina is homozygous for Pak20 while other tissues are heterozygous (clones generated using the "eyFLP" system); mosaic larvae show a disorganised pattern of photoreceptor axon projection and 92% of brain hemispheres of mosaic adults show a "medulla bypass" phenotype.

        (Newsome et al., 2000)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Enhancer of
        Statement
        Reference

        Pak[+]/Pak20 is an enhancer of phenotype of trioS138606/trio1

        (Newsome et al., 2000)

        Pak[+]/Pak20 is an enhancer of photoreceptor cell & axon phenotype of trioS138606/trio1

        (Newsome et al., 2000)

        Pak[+]/Pak20 is an enhancer of phenotype of trioS138606/trio8

        (Newsome et al., 2000)

        Pak[+]/Pak20 is an enhancer of photoreceptor cell & axon phenotype of trioS138606/trio8

        (Newsome et al., 2000)
        Other
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Gitex21C Pak20 zygotic double mutant embryos show muscle targeting defects that are not seen in either zygotic mutant alone, and the double mutant embryos show a general disorganisation of their muscle pattern.

        (Bahri et al., 2009)

        The frequency of trioS138606/trio1 or trioS138606/trio8 animals showing defects in photoreceptor axon projection (having a "medulla bypass" phenotype) is enhanced if they are also heterozygous for Pak20.

        (Newsome et al., 2000)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        Pak20
        dpak20
        (Bahri et al., 2009)
        dpak20
        (Bahri et al., 2009)
        Name Synonyms
        Secondary FlyBase IDs
          References (3)