FlyBase Allele Report: Dmel\trio[1]

General Information

Symbol

Dmel\trio¹

Species

D. melanogaster

Name

FlyBase ID

FBal0117311

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ball et al., 2010)

Mutagen

ethyl methanesulfonate

(Newsome et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: W315term.

(Newsome et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:1,028,291..1,028,291 [-]

Nucleotide change:

G1028291A

Amino acid change:

W315term | trio-PA; W315term | trio-PC

Reported amino acid change:

W315term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Newsome et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with trio^6A)

(Ball et al., 2010)

abnormal neuroanatomy (with trio^123.4)

(Song and Giniger, 2011)

abnormal neuroanatomy | adult stage | somatic clone

(Holbrook et al., 2012)

abnormal neuroanatomy | embryonic stage (with trio⁸)

(Song et al., 2010)

lethal | recessive

(Newsome et al., 2000)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with trio^6A)

(Ball et al., 2010)

embryonic somatic muscle cell (with trio⁸)

(Hakeda-Suzuki et al., 2002)

larval intersegmental nerve branch ISNb of A1-7 (with trio⁸)

(Song et al., 2010)

larval intersegmental nerve branch ISNb of A1-7 (with trio^123.4)

(Song and Giniger, 2011)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with trio⁸)

(Kannan et al., 2017)

larval segmental nerve branch SNa of A1-7 (with trio⁸)

(Song et al., 2010)

NMJ bouton | third instar larval stage (with trio^6A)

(Ball et al., 2010)

photoreceptor cell & axon (with trio^S138606)

(Newsome et al., 2000)

photoreceptor cell & axon | somatic clone

(Newsome et al., 2000)

photoreceptor cell R7 | somatic clone

(Holbrook et al., 2012)

Detailed Description

Statement

Reference

Larval muscle 4 neuromuscular junctions in trio¹ heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

(Migh et al., 2018)

trio⁸/trio¹ embryos display stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 in >65% of hemisegments examined.

(Kannan et al., 2017)

Axon terminals of homozygous R7 photoreceptor cell clones sometimes fail to contact the M6 layer of the medulla. Those axons that do contact M6 sometimes project thin extensions beyond M6.

(Holbrook et al., 2012)

trio¹/trio^123.4 mutants display a specific axonal defect in ISNb, 'stalling' in the middle of the target field.

(Song and Giniger, 2011)

trio¹/trio^6A third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls.

(Ball et al., 2010)

trio¹/trio⁸ embryos show defects in motor axon patterning; ISNb motor axons stall and fail to innervate their most distal target and the embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target.

(Song et al., 2010)

Dorsal closure occurs normally in trio¹/trio⁸ embryos derived from trio¹/trio¹ mothers. Myoblast fusion appears complete, but myotubes often fail to attach correctly to the epidermis.

(Hakeda-Suzuki et al., 2002)

When mutant somatic clones are made in the border cells no effect is seen.

(Duchek et al., 2001)

Defects in pathfinding by photoreceptor axons are seen in mosaic larvae in which virtually the entire retina is homozygous for trio¹ while other tissues are heterozygous (clones generated using the "eyFLP" system); the photoreceptor axons appear to extend normally into the brain but fail to elaborate smooth retinotopic arrays in the lamina and medulla. Less than 10% of trio^S138606/trio¹ animals show defects in photoreceptor axon projection, having a "medulla bypass" phenotype.

(Newsome et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

trio¹/trio[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Dab¹

(Song et al., 2010)

trio¹/trio[+] is an enhancer of abnormal neuroanatomy | embryonic stage | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

Suppressor of

Statement

Reference

trio¹/trio[+] is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

trio^S138606/trio¹ has phenotype, enhanceable by Pak[+]/Pak¹⁶

(Newsome et al., 2000)

trio^S138606/trio¹ has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak¹⁶

(Newsome et al., 2000)

trio^S138606/trio¹ has phenotype, enhanceable by Pak[+]/Pak²⁰

(Newsome et al., 2000)

trio^S138606/trio¹ has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak²⁰

(Newsome et al., 2000)

Enhancer of

Statement

Reference

trio¹/trio[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

trio¹/trio[+] is an enhancer of larval segmental nerve branch SNa of A1-7 | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

trio¹/trio[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of Dab¹

(Song et al., 2010)

trio¹/trio[+] is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of Dab¹

(Song et al., 2010)

trio¹/trio[+] is an enhancer of photoreceptor cell R7 & axon phenotype of Lar^5.5/Lar²¹²⁷

(Maurel-Zaffran et al., 2001)

Suppressor of

Statement

Reference

trio¹/trio[+] is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Larval muscle 4 neuromuscular junctions in trio¹, DAAM^Ex68 double heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

(Migh et al., 2018)

The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab¹/Dab² females and having Dab¹ as the paternally derived copy of Dab) is increased by trio¹/+ to 78% and 80% respectively.

The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen Dab¹ homozygous embryos is increased by trio¹/+ to 72% and 35% respectively.

(Song et al., 2010)

The frequency of trio^S138606/trio¹ animals showing defects in photoreceptor axon projection (having a "medulla bypass" phenotype) is enhanced if they are also heterozygous for either Pak¹⁶ or Pak²⁰.

(Newsome et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of trio^{GEF1mu.Scer\UAS} (under the control of Scer\GAL4^elav-C155) fails to rescue the ISNb axonal phenotypes of trio¹/trio^123.4 mutants.

Expression of trio^{GEF2mu.Scer\UAS} (under the control of Scer\GAL4^elav-C155) rescues the ISNb axonal phenotypes found in trio¹/trio^123.4 mutants.

Expression of trio^Scer\UAS.cBa (under the control of Scer\GAL4^elav-C155) rescues the ISNb axonal phenotypes found in trio¹/trio^123.4 mutants.

(Song and Giniger, 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9129

y¹ w^*; trio¹ P{FRT(w^hs)}2A/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trio¹

(Shcherbata et al., 2007, Duchek et al., 2001)

trio¹

(Cheong et al., 2020, Migh et al., 2018, Kannan et al., 2017, Holbrook et al., 2012, Goossens et al., 2011, Song and Giniger, 2011, Ball et al., 2010, Malartre et al., 2010, Song et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (16)

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