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General Information
Symbol
Dmel\trio1
Species
D. melanogaster
Name
FlyBase ID
FBal0117311
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G1028291A

Amino acid change:

W315term | trio-PA; W315term | trio-PC

Reported amino acid change:

W315term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W315term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval muscle 4 neuromuscular junctions in trio1 heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

trio8/trio1 embryos display stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 in >65% of hemisegments examined.

Axon terminals of homozygous R7 photoreceptor cell clones sometimes fail to contact the M6 layer of the medulla. Those axons that do contact M6 sometimes project thin extensions beyond M6.

trio1/trio123.4 mutants display a specific axonal defect in ISNb, 'stalling' in the middle of the target field.

trio1/trio6A third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls.

trio1/trio8 embryos show defects in motor axon patterning; ISNb motor axons stall and fail to innervate their most distal target and the embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target.

Dorsal closure occurs normally in trio1/trio8 embryos derived from trio1/trio1 mothers. Myoblast fusion appears complete, but myotubes often fail to attach correctly to the epidermis.

When mutant somatic clones are made in the border cells no effect is seen.

Defects in pathfinding by photoreceptor axons are seen in mosaic larvae in which virtually the entire retina is homozygous for trio1 while other tissues are heterozygous (clones generated using the "eyFLP" system); the photoreceptor axons appear to extend normally into the brain but fail to elaborate smooth retinotopic arrays in the lamina and medulla. Less than 10% of trioS138606/trio1 animals show defects in photoreceptor axon projection, having a "medulla bypass" phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

trio1/trio[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Dab1

Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

trioS138606/trio1 has phenotype, enhanceable by Pak[+]/Pak16

trioS138606/trio1 has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak16

trioS138606/trio1 has phenotype, enhanceable by Pak[+]/Pak20

trioS138606/trio1 has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak20

Enhancer of
Statement
Reference

trio1/trio[+] is an enhancer of photoreceptor cell R7 & axon phenotype of Lar5.5/Lar2127

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Larval muscle 4 neuromuscular junctions in trio1, DAAMEx68 double heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by trio1/+ to 78% and 80% respectively.

The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen Dab1 homozygous embryos is increased by trio1/+ to 72% and 35% respectively.

The frequency of trioS138606/trio1 animals showing defects in photoreceptor axon projection (having a "medulla bypass" phenotype) is enhanced if they are also heterozygous for either Pak16 or Pak20.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of trioGEF1mu.Scer\UAS (under the control of Scer\GAL4elav-C155) fails to rescue the ISNb axonal phenotypes of trio1/trio123.4 mutants.

Expression of trioGEF2mu.Scer\UAS (under the control of Scer\GAL4elav-C155) rescues the ISNb axonal phenotypes found in trio1/trio123.4 mutants.

Expression of trioScer\UAS.cBa (under the control of Scer\GAL4elav-C155) rescues the ISNb axonal phenotypes found in trio1/trio123.4 mutants.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (16)