FlyBase Allele Report: Dmel\trio[6A]

General Information

Symbol

Dmel\trio^6A

Species

D. melanogaster

Name

FlyBase ID

FBal0117304

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ball et al., 2010)

Mutagen

ethyl methanesulfonate

(Bateman et al., 2000)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with trio¹)

(Ball et al., 2010)

abnormal neuroanatomy (with trio^S137203)

(Ball et al., 2010)

abnormal neuroanatomy | embryonic stage 17

(Malartre et al., 2010)

lethal | recessive

(Bateman et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

trio¹/trio^6A third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls.

trio^S137203/trio^6A third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls. The sizes of evoked excitatory junctional potentials (EJPs), miniature excitatory junctional potentials and the quantal content do not differ significantly from controls.

(Ball et al., 2010)

trio^6A homozygous stage 17 embryos exhibit interrupted axon tracts.

(Malartre et al., 2010)

Heterozygotes with Df(3L)Ar12-1 rarely survive to pupal stages. Mutants show a short stop phenotype, with defects in the ability of nerve branches to reach target muscles. ISNb (where the effect is most pronounced) and SNa are most sensitive to the loss of trio activity. In addition, there are occasional defects in epidermal muscle attachment sites. The CNS also shows pathfinding errors resulting in breaks in the longitudinal connectives. The axons often fail to traverse the segment boundary.

(Bateman et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

trio^6A has abnormal neuroanatomy | embryonic stage 17 phenotype, enhanceable by Vav²

(Malartre et al., 2010)

Enhancer of

Statement

Reference

trio^6A is an enhancer of abnormal neuroanatomy | embryonic stage 17 phenotype of Vav²

(Malartre et al., 2010)

Suppressor of

Statement

Reference

trio^6A/trio[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

trio^6A/trio[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

trio^6A/trio^S137203 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^6A/trio^S137203 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^BG380, sax^A.UAS.cBa, tkv^{Q199D.UAS.Tag:HA}

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

trio^6A/trio^S137203 is a non-suppressor of abnormal size | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

trio^6A/trio^S137203 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Df(2R)en-SFX31, trio^6A has abnormal neuroanatomy phenotype

(Joly et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

trio^6A has larval longitudinal connective | embryonic stage 17 phenotype, enhanceable by Vav²

(Malartre et al., 2010)

Enhancer of

Statement

Reference

trio^6A is an enhancer of larval longitudinal connective | embryonic stage 17 phenotype of Vav²

(Malartre et al., 2010)

trio^6A is an enhancer of commissure | embryonic stage 17 phenotype of Vav²

(Malartre et al., 2010)

Suppressor of

Statement

Reference

trio^6A/trio[+] is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

NOT Suppressor of

Statement

Reference

trio^6A/trio^S137203 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

trio^6A/trio^S137203 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Df(2R)en-SFX31, trio^6A has larval ventral nerve cord phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, trio^6A has larval anterior commissure phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, trio^6A has larval posterior commissure phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, trio^6A has lateral tract phenotype

(Joly et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

trio^S137203/trio^6A does not suppress the larval neuromuscular junction overgrowth and increase in bouton number seen when Rac1^V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4^BG380.

trio^S137203/trio^6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1^Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4^OK6.

trio^S137203/trio^6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when sax^{A.Scer\UAS.cBa} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1} are expressed in motor neurons under the control of Scer\GAL4^BG380.

(Ball et al., 2010)

A large proportion of vav² trio^6A double mutant embryos exhibit gross patterning defects that make examination of axogenesis defects difficult. However in double mutant embryos that display less severe morphological defects the midline crossing phenotypes are enhanced compared to single mutants.

(Malartre et al., 2010)

Stage 15 transheterozygous Df(2R)en-SFX31/trio^6A embryos exhibit defects in central nervous system scaffolding with gaps in longitudinal tracts, loss of posterior commissures, or fusion of commissures in about 64% of the segments.

Stage 15 transheterozygous en^E/trio^6A embryos exhibit defects in central nervous system scaffolding with gaps in longitudinal tracts, loss of posterior commissures, or fusion of commissures in about 12% of the segments.

(Joly et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

trio^6A/trio^S137203 is rescued by trio^UAS.cBa/Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^6A/trio^S137203 is rescued by Scer\GAL4^{elav.Switch.PO}/trio^UAS.cBa

(Ball et al., 2010)

Partially rescued by

trio^6A/Df(3L)Ar12-1 is partially rescued by Scer\GAL4^elav-C155/trio^UAS.cBa

(Bateman et al., 2000)

Not rescued by

trio^6A/trio^S137203 is not rescued by Scer\GAL4^G14/trio^UAS.cBa

(Ball et al., 2010)

Comments

Expression of trio^Scer\UAS.cBa in neurons under the control of Scer\GAL4^OK6 rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae. Expression of trio^Scer\UAS.cBa in muscles using Scer\GAL4^G14 is unable to rescue the phenotype.

Expression of trio^Scer\UAS.cBa only in the larval stages using the neuronal driver Scer\GAL4^{elav.Switch.PO} rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae.

(Ball et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8595

w^*; trio^6A/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Trio⁸⁵⁹⁵

(Shao et al., 2017)

trio^6A

(Hildebrand et al., 2021, Long et al., 2016, Ball et al., 2010, Malartre et al., 2010, Joly et al., 2007)

trio^S6A

(Spinner et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (8)

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