FlyBase Allele Report: Dmel\Vav[2]

General Information

Symbol

Dmel\Vav²

Species

D. melanogaster

Name

FlyBase ID

FBal0263789

Feature type

allele

Associated gene

Dmel\Vav

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Malartre et al., 2010)

Mutagen

Delta2-3 transposase

(Malartre et al., 2010)

Progenitor genotype

Vav^KG02022

(Malartre et al., 2010)

Cytology

Description

Imprecise excision of vav^KG02022 results in a 1532p deletion of the vav locus. Neighbouring genes CG8010 and rictor are intact.

(Malartre et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

commissure | embryonic stage 17

(Malartre et al., 2010)

cone cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

ellipsoid body | adult stage | male limited

(Malartre et al., 2010)

embryonic/larval neuromuscular junction | larval stage

(Kim et al., 2024)

eye disc | third instar larval stage

(Martín-Bermudo et al., 2015)

eye photoreceptor cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

interommatidial cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

larval longitudinal connective | embryonic stage 17

(Malartre et al., 2010)

NMJ bouton | increased number | larval stage | male

(Kim et al., 2024)

ommatidial precursor cluster | third instar larval stage

(Martín-Bermudo et al., 2015)

photoreceptor cell R7 | increased number | third instar larval stage

(Martín-Bermudo et al., 2015)

photoreceptor cell R7 | pupal stage | increased number

(Martín-Bermudo et al., 2015)

photoreceptor neuron | increased number | third instar larval stage

(Martín-Bermudo et al., 2015)

primary pigment cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

retina | pupal stage

(Martín-Bermudo et al., 2015)

Detailed Description

Statement

Reference

The regular hexagonal lattice of pupal retina is disorganized in homozygous Vav² mutants and contain extra cells of all types (inter-ommatidial cells, primary pigment cells as well as cone cells).

Vav² homozygous mutants display extra photoreceptor cells in third instar larval eye disc (R7 and R8) as well as in the majority of ommatidia (supernumerary R7 photoreceptor cells) in a pupal retina. Precocious recruitment of R7 cells is observed in Vav² mutant eye discs compared to wild-type controls.

The presence of supernumerary accessory cells in Vav² homozygous pupal ommatidia does not correlate with the presence of extra R7 photoreceptor cells and the numbers of extra cone cells also do not correlate with the number of primary pigment cells.

Vav² mutants display defect in ommatidial cluster rotation in third instar larval eye disc.

Cells in Vav² homozygous somatic clones display defects in adherens junction dynamics and excessive cell movements (revealead by live imaging) which result in a patterning delay. The dynamics defects correlate with the recruitment of extra primary pigment cells.

(Martín-Bermudo et al., 2015)

The majority of vav² homozygous mutants die at the pupal stages but male escapers exist. The escapers are wild type in appearance but exhibit locomotor defects and die shortly after eclosion. They have impaired spontaneous activity and a "shaking phenotype".

vav² pupae that fail to eclose contain fully developed flies that exhibit severe locomotor defects.

Defects in axon projection are seen in approximately 14% of stage 17 vav² embryos. On average two longitudinal axons are misrouted across the midline in each embryo. This phenotype is not enhanced when the maternal vav product is absent.

The midline glia is present in vav² embryos.

63% of vav² male homozygotes exhibit axon growth defects in the adult ellipsoid body, although the severity of the phenotype varies. In milder mutants only the external ring remains open whereas in more severe mutants both rings are open.

(Malartre et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Vav² has increased cell number | pupal stage phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Vav² has abnormal neuroanatomy | embryonic stage 17 phenotype, enhanceable by trio^6A

(Malartre et al., 2010)

Vav² has abnormal neuroanatomy | embryonic stage 17 phenotype, enhanceable by Sos^e2H

(Malartre et al., 2010)

Suppressed by

Statement

Reference

Vav² has increased cell number | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

NOT suppressed by

Statement

Reference

Vav² has partially lethal - majority die | male limited | pupal stage phenotype, non-suppressible by trio^UAS.cBa/Scer\GAL4^arm.PS

(Malartre et al., 2010)

Vav² has partially lethal - majority die | male limited | pupal stage phenotype, non-suppressible by trio^UAS.cBa/Scer\GAL4^αTub84B.PL

(Malartre et al., 2010)

Enhancer of

Statement

Reference

Vav² is an enhancer of abnormal neuroanatomy | embryonic stage 17 phenotype of trio^6A

(Malartre et al., 2010)

Vav² is an enhancer of abnormal neuroanatomy | embryonic stage 17 phenotype of Sos^e2H

(Malartre et al., 2010)

Suppressor of

Statement

Reference

Vav² is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, aos^UAS.cUa

(Martín-Bermudo et al., 2015)

Other

Statement

Reference

Rap1^rvB1, Vav² has abnormal neurophysiology | larval stage phenotype

(Kim et al., 2024)

Adcy1^rut-1, Vav² has abnormal neurophysiology | larval stage phenotype

(Kim et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

Vav² has retina | pupal stage phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Vav² has primary pigment cell | pupal stage | increased number phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Vav² has larval longitudinal connective | embryonic stage 17 phenotype, enhanceable by trio^6A

(Malartre et al., 2010)

Vav² has commissure | embryonic stage 17 phenotype, enhanceable by trio^6A

(Malartre et al., 2010)

Vav² has larval longitudinal connective | embryonic stage 17 phenotype, enhanceable by Sos^e2H

(Malartre et al., 2010)

Vav² has commissure | embryonic stage 17 phenotype, enhanceable by Sos^e2H

(Malartre et al., 2010)

NOT Enhanced by

Statement

Reference

Vav² has NMJ bouton | larval stage | increased number phenotype, non-enhanceable by Scer\GAL4^elav-C155/Rap1^{V12.UAS.Tag:MYC}

(Kim et al., 2024)

Vav² has cone cell | pupal stage | increased number phenotype, non-enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Suppressed by

Statement

Reference

Vav² has retina | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

Vav² has cone cell | increased number | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

NOT suppressed by

Statement

Reference

Vav² has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Rap1^{V12.UAS.Tag:MYC}

(Kim et al., 2024)

Vav² has primary pigment cell | increased number | pupal stage phenotype, non-suppressible by spi¹

(Martín-Bermudo et al., 2015)

Enhancer of

Statement

Reference

Vav²/Vav[+] is an enhancer of retina | pupal stage phenotype of Scer\GAL4^GMR.PU, cindr^{RNAi.PC.PD.UAS}

(Martín-Bermudo et al., 2015)

Vav²/Vav[+] is an enhancer of primary pigment cell | pupal stage | increased number phenotype of Scer\GAL4^GMR.PU, cindr^{RNAi.PC.PD.UAS}

(Martín-Bermudo et al., 2015)

Vav² is an enhancer of larval longitudinal connective | embryonic stage 17 phenotype of Sos^e2H

(Malartre et al., 2010)

Vav² is an enhancer of commissure | embryonic stage 17 phenotype of Sos^e2H

(Malartre et al., 2010)

Vav² is an enhancer of larval longitudinal connective | embryonic stage 17 phenotype of trio^6A

(Malartre et al., 2010)

Suppressor of

Statement

Reference

Vav²/Vav[+] is a suppressor of NMJ bouton | larval stage | decreased number phenotype of Rap1^{V12.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Kim et al., 2024)

Vav² is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, aos^UAS.cUa

(Martín-Bermudo et al., 2015)

Other

Statement

Reference

Abl⁴, Vav² has embryonic/larval neuromuscular junction | larval stage phenotype

(Kim et al., 2024)

Abl⁴, Vav² has NMJ bouton | increased number | larval stage phenotype

(Kim et al., 2024)

Abl⁴, Vav² has macropinosome | larval stage phenotype

(Kim et al., 2024)

Rap1^rvB1, Vav² has embryonic/larval neuromuscular junction | larval stage phenotype

(Kim et al., 2024)

Rap1^rvB1, Vav² has NMJ bouton | increased number | larval stage phenotype

(Kim et al., 2024)

Rap1^rvB1, Vav² has macropinosome | larval stage phenotype

(Kim et al., 2024)

Adcy1^rut-1, Vav² has embryonic/larval neuromuscular junction | larval stage phenotype

(Kim et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary cone cells in pupal retina characteristic for Vav² mutants is almost completely suppressed by combination with spi¹ in heterozygous state but the increased number of primary pigment cells remains unchanged.

The disorganised ommatidial lattice and supernumerary primary pigment cells phenotype characteristic for Vav² homozygotes is strongly enhanced by combination with shg^k03401 in heterozygous state but the proportion of ommatidia with extra cone cells is not further increased.

The patterning defects and mild supernumerary primary pigment cells phenotype observed in pupal retina of flies expressing cindr^{dsRNA.PC.PD.Scer\UAS} under the control of Scer\GAL4^GMR.PU can be significantly enhanced by combination with Vav² in heterozygous state.

The small and rough adult eye phenotype seen in flies expressing aos^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PU can be partially restored by combination with Vav².

(Martín-Bermudo et al., 2015)

Expression of trio^Scer\UAS.cBa under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver cannot suppress the semi-lethality seen in vav² male homozygotes.

A large proportion of vav² trio^6A double mutant embryos exhibit gross patterning defects that make examination of axogenesis defects difficult. However in double mutant embryos that display less severe morphological defects the midline crossing phenotypes are enhanced compared to single mutants.

Homozygous Sos^e2H enhances the axon tract phenotypes seen in vav² homozygous mutant embryos, with an increased number of midline crosses per embryo. No other CNS defects are observed.

(Malartre et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vav² is rescued by Scer\GAL4^arm.PS/Vav^UAS.Tag:HA

(Malartre et al., 2010)

Vav² is rescued by Vav^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Malartre et al., 2010)

Partially rescued by

Vav² is partially rescued by Scer\GAL4^arm.PS/Vav^UAS.Tag:HA

(Malartre et al., 2010)

Comments

Expression of vav^{Scer\UAS.T:Ivir\HA1} under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver rescues the semi-lethality seen in vav² male homozygotes.

Expression of vav^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the midline crossing phenotype seen in vav² homozygotes.

(Malartre et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98868

y¹ w^* Vav²/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Vav²

(Kim et al., 2024, Park et al., 2022)

vav²

(Martín-Bermudo et al., 2015, Malartre et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (4)

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