FlyBase Allele Report: Dmel\chb[EP3403]

General Information

Symbol

Dmel\chb^EP3403

Species

D. melanogaster

Name

FlyBase ID

FBal0118303

Feature type

allele

Associated gene

Dmel\chb

Associated Insertion(s)

P{EP}chb^EP3403

Carried in Construct

Also Known As

EP(3)3403

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Huang and Rubin, 2000, Lemos et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}chb^EP3403

Cytology

Description

The orientation of the P{EP} element is the same as the direction of chb transcription.

(Shulman and Feany, 2003)

The P{EP} insertion maps to the 5' end of the long chb transcription unit, 2317bp upstream of the predicted ATG.

(Lemos et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:21,175,814..21,175,814 [+]

(Lee et al., 2004, Shulman and Feany, 2003, Gene Disruption Project members, 2001-, Huang and Rubin, 2000, Lemos et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Lemos et al., 2000)

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Lee et al., 2004)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001, Huang and Rubin, 2000)

lethal - all die before end of pupal stage (with Df(3L)31A)

(Lemos et al., 2000)

some die during pupal stage (with Df(3L)31A)

(Lemos et al., 2000)

viable

(Huang and Rubin, 2000)

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval neuron, with Scer\GAL4^elav.PLu

(Lee et al., 2004)

mitotic cell cycle

(Lemos et al., 2000)

Detailed Description

Statement

Reference

Embryos expressing chb^EP3403 under the control of Scer\GAL4^elav.PLu show a low but significant number of axons ectopically crossing the midline.

(Lee et al., 2004)

Mutant larvae expressing chb^EP3403 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing chb^EP3403 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

Mutant larval brains have a mitotic index significantly higher than wild-type, (1.94 compared to 1.14), the majority of cells are arrested in metaphase or prometaphase, polyploid cells, cells with abnormal anaphases and circular mitotic figures are also seen.

(Lemos et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, chb^EP3403 has abnormal neuroanatomy phenotype, enhanceable by Abl^UAS.cFa

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb^EP3403 is an enhancer of abnormal neuroanatomy phenotype of Abl^UAS.cFa, Scer\GAL4^elav.PLu

(Lee et al., 2004)

chb^EP3403, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Scer\GAL4^hs.2sev/chb^EP3403 is an enhancer of visible phenotype of ksr::tor^{ΔNksr.hs.sev}

(Huang and Rubin, 2000)

NOT Enhancer of

Statement

Reference

chb^EP3403, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev/chb^EP3403 is a suppressor of visible phenotype of Ras85D^V12.sev

(Huang and Rubin, 2000)

NOT Suppressor of

Statement

Reference

chb^EP3403, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, chb^EP3403 has larval neuron phenotype, enhanceable by Abl^UAS.cFa

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb^EP3403 is an enhancer of larval intersegmental nerve phenotype of Abl^UAS.cFa, Scer\GAL4^elav.PLu

(Lee et al., 2004)

chb^EP3403 is an enhancer of larval neuron phenotype of Abl^UAS.cFa, Scer\GAL4^elav.PLu

(Lee et al., 2004)

chb^EP3403, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

chb^EP3403, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Scer\GAL4^hs.2sev/chb^EP3403 is an enhancer of eye phenotype of ksr::tor^{ΔNksr.hs.sev}

(Huang and Rubin, 2000)

NOT Enhancer of

Statement

Reference

chb^EP3403, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev/chb^EP3403 is a suppressor of eye phenotype of Ras85D^V12.sev

(Huang and Rubin, 2000)

NOT Suppressor of

Statement

Reference

chb^EP3403, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of chb^EP3403 and Abl^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu in embryos results in an increased frequency of axons ectopically crossing the midline compared to embryos expressing either chb^EP3403 or Abl^Scer\UAS.cFa singly under the control of Scer\GAL4^elav.PLu. Co-expression of chb^EP3403 enhances the expressivity of the ISNb axons phenotype caused by expression of Abl^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu, although the effect is subtle.

(Lee et al., 2004)

Strong enhancer of ksr::tor^{ΔNksr.hs.sev} and moderate suppressor of Ras85D^V12.sev, when expression is driven by Scer\GAL4^hs.2sev.

(Huang and Rubin, 2000)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by chb^EP3403.

(Shulman and Feany, 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The effects on viability, mitotic phenotype and mitotic progression suggest an allelic series: chb^EP3515 < chb^v40 < chb^EP3403 < chb^P4.

(Lemos et al., 2000)

Precise excision of the P{EP} element reverts the modifying effect of chb^EP3403 on the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Shulman and Feany, 2003)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF195498

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAG28470

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

chb^EP3403

mast3

(Lemos et al., 2000)

mast^P3

(Lemos et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (6)

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