FlyBase Allele Report: Dmel\neur[GAL4-A101]

General Information

Symbol

Dmel\neur^GAL4-A101

Species

D. melanogaster

Name

FlyBase ID

FBal0120429

Feature type

allele

Associated gene

Dmel\neur

Associated Insertion(s)

P{GawB}neur^GAL4-A101

Carried in Construct

Also Known As

neur-Gal4, neuralized-GAL4, neu-gal4, neuralised-GAL4, neurGal4

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

gene conversion

(Ayyub, 2001.12.14, Jhaveri et al., 2000, Reddy et al., 1999)

P-element activity

(Ayyub, 2001.12.14)

Progenitor genotype

neur^A101

(Ayyub, 2001.12.14, Jhaveri et al., 2000, Reddy et al., 1999)

Associated Insertion(s)

P{GawB}neur^GAL4-A101

Cytology

Description

The P{lArB} element in neur^A101 has been replaced with the P{GawB} element from P{GawB}SG18.1 by P\T-mediated conversion.

(Ayyub, 2001.12.14)

The P{lArB} sequences of the neur^A101 insertion have been replaced with P{GAL4} sequences.

(Jhaveri et al., 2000)

The P{lArB} element of neur^A101 has been replaced with a P{GAL4} element.

(Reddy et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory

(Pavlopoulos et al., 2008)

lethal | recessive

(Skwarek et al., 2007)

neurogenic phenotype | embryonic stage | recessive

(Liu et al., 2012, Skwarek et al., 2007)

Phenotype Manifest In

larval nervous system

(Liu et al., 2012, Skwarek et al., 2007)

Detailed Description

Statement

Reference

neur^GAL4-A101 heterozygotes exhibit learning and 9-minute memory abilities that appear identical to wild-type controls. However, these animals display significantly reduced 260-minute memory. This memory deficit is detectable 24 hours later. These mutants do not exhibit deficiencies in the perception of conditioned stimuli (CS) and unconditioned stimuli (US) or detectable aberrations in the architecture of the brain and the mushroom bodies.

(Pavlopoulos et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

neur^GAL4-A101 is partially rescued by Scer\GAL4^{neur-GAL4-A101}/neur^{UAS.PA.Tag:V5}

(Liu et al., 2012)

neur^GAL4-A101 is partially rescued by Scer\GAL4^{neur-GAL4-A101}/neur^{UAS.PA.Tag:V5}

(Skwarek et al., 2007)

Not rescued by

neur^GAL4-A101 is not rescued by Scer\GAL4^{neur-GAL4-A101}/neur^{G430E.UAS.PA.Tag:V5}

(Liu et al., 2012)

neur^GAL4-A101 is not rescued by Scer\GAL4^{neur-GAL4-A101}/neur^{5Q.UAS.PA.Tag:V5}

(Skwarek et al., 2007)

Comments

Expression of neur^{Scer\UAS.PA.T:SV5\V5} under the control of Scer\GAL4^{neur-GAL4-A101} strongly rescues the neurogenic phenotype of homozygous neur^GAL4-A101 embryos.

Expression of neur^{G430E.Scer\UAS.PA.T:SV5\V5} under the control of Scer\GAL4^{neur-GAL4-A101} fails to rescue the neurogenic phenotype of homozygous neur^GAL4-A101 embryos.

Expression of neur^{ΔNH2.Scer\UAS.PA.T:SV5\V5} under the control of Scer\GAL4^{neur-GAL4-A101} may have some residual rescue activity of the neurogenic phenotype of homozygous neur^GAL4-A101 embryos.

(Liu et al., 2012)

Expression of neur^{Scer\UAS.PA.T:SV5\V5} under the control of Scer\GAL4^{neur-GAL4-A101} strongly rescues the neurogenic phenotype of homozygous neur^GAL4-A101 embryos.

Expression of neur^{5Q.Scer\UAS.PA.T:SV5\V5} under the control of Scer\GAL4^{neur-GAL4-A101} fails to rescue the neurogenic phenotype of homozygous neur^GAL4-A101 embryos.

(Skwarek et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6393

w¹¹¹⁸; P{GawB}neur^GAL4-A101 Kg^V/TM3, Sb¹

Kyoto

108764

w¹¹¹⁸; P{GawB}neur^GAL4-A101 Kg^V/TM3, Sb¹