FlyBase Allele Report: Dmel\fu[mH63]

General Information

Symbol

Dmel\fu^mH63

Species

D. melanogaster

Name

FlyBase ID

FBal0120493

Feature type

allele

Associated gene

Dmel\fu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Therond et al., 1993, Busson et al., 1988)

hypomorphic allele - genetic evidence

(Liu et al., 2007)

loss of function allele

(Mullor and Guerrero, 2000)

Mutagen

ethyl methanesulfonate

(Therond et al., 1996, Preat et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: A147T. Residue is located 2 positions downstream of the DFG triplet.

(Therond et al., 1996)

Amino acid replacement: A?T. Mutation is in the kinase domain.

(Preat et al., 1993)

restriction map normal

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:18,658,350..18,658,350 [+]

Nucleotide change:

G18658350A

Reported nucleotide change:

G1414A

Amino acid change:

A147T | fu-PA

Reported amino acid change:

A147T

(Preat et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage | recessive

(Therond et al., 1993)

lethal - all die before end of pupal stage

(Busson et al., 1988)

lethal - all die during embryonic stage | germline clone | recessive

(Deshpande et al., 2001)

some die during embryonic stage | recessive

(Deshpande et al., 2001)

some die during pharate adult stage | recessive

(Therond et al., 1993)

some die during pupal stage

(Busson et al., 1988)

visible

(Therond et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

Polar follicle cell specification occurs in clones induced in somatic stem cells and their descendents. Relatively large somatic clones that include the anterior region of the follicular epithelium of an egg chamber are associated with the production of egg chambers with abnormal numbers of germline cells.

(Besse et al., 2002)

Germ cells in embryos lacking maternal fu function (derived from females carrying fu^mH63 germline clones and fertilised with a wild-type sperm) migrate towards the somatic gonadal precursor cells but fail to properly associate with them and instead scatter through the posterior half of the embryo. These embryos do not hatch.

(Deshpande et al., 2001)

Mutant embryos derived from homozygous germline clones lack Bolwig's organs.

(Suzuki and Saigo, 2000)

fu^mH63 mutant clones at the AP border of the wing invade posterior compartment territory and induce a central vein 3 decorated with sensillae at the position normally occupied by vein 4. Wing morphology is not affected in clones outside the region usually affected by hh signalling.

(Ohlmeyer and Kalderon, 1998)

Homozygotes display a strong fu wing phenotype.

(Therond et al., 1996)

Pharates die with a strong fused wing phenotype.

(Therond et al., 1993)

Homozygous and hemizygous females and hemizygous males die in pupal stage; lethality suppressible by Su(fu). fu^mH63/fu⁴¹ and fu^mH63/Y have all segments entirely duplicated; lack mouth hooks and some have no head. fu^mH63/+ embryos from such clones exhibit very low paternal rescue; small correction of segmental phenotype, low hatch and preadult mortality (Busson et al. 1988). pupal lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

fu^mH63, ptc^S2 has increased occurrence of cell division | somatic clone | oogenesis phenotype

(Zhang and Kalderon, 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

fu^mH63 has Bolwig organ | germline clone phenotype, suppressible by hh^UAS.cKa/Scer\GAL4^da.G32

(Suzuki and Saigo, 2000)

fu^mH63 has phenotype, suppressible by Su(fu)^LP

(Preat et al., 1993, Preat, 1992)

fu^mH63 has phenotype, suppressible by Su(fu)^12d

(Preat, 1992)

Enhancer of

Statement

Reference

fu^mH63 is an enhancer of wing phenotype of ptc^S2

(Ohlmeyer and Kalderon, 1998)

fu^mH63 is an enhancer of wing vein phenotype of ptc^S2

(Ohlmeyer and Kalderon, 1998)

NOT Enhancer of

Statement

Reference

fu^mH63 is a non-enhancer of wing phenotype of Pka-C1^B3

(Ohlmeyer and Kalderon, 1998)

NOT Suppressor of

Statement

Reference

fu^mH63 is a non-suppressor of wing phenotype of Pka-C1^B3

(Ohlmeyer and Kalderon, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The lack of Bolwig's organs seen in embryos derived from homozygous germline is partially rescued by expression of hh^Scer\UAS.cKa under the control of Scer\GAL4^da.G32.

(Suzuki and Saigo, 2000)

Somatic stem cell clones in the ovary homozygous for both fu^mH63 and ptc^S2 show only very mild overproliferation.

(Zhang and Kalderon, 2000)

Wing phenotype of Pka-C1^B3 mutant clones is unaffected by fu^mH63. ptc^S2, fu^mH63 double mutant clones still retain some pattern organising activity in wings.

(Ohlmeyer and Kalderon, 1998)

When in combination with Su(fu)^LP heterozygotes individuals display a partially suppressed fu phenotype. When in combination with cos³ individuals display no cos phenotype.

(Preat et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fu^mH63 is rescued by fu^+t5.1

(Therond et al., 1993)

fu^mH63 is rescued by fu^+t7.4

(Therond et al., 1993)

Comments

Lethality is rescued when in combination with either fu^+t5.1 or fu^+t7.4, individuals display wild type wings.

(Therond et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7207

fu^mH63/FM6

Notes on Origin

Discoverer

Hanratty.

Comments

Class I fu allele.

(Preat et al., 1993)

Class I mutation based on interaction with Su(fu)^LP, suppression of embryonic and adult phenotype.

(Therond et al., 1996)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

S69166

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAB29841

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

fu²⁹

fu³¹

fu^MH63

(Zhou et al., 2006, Methot and Basler, 2000, van den Heuvel et al., 1993)

fu^mH63

(Little et al., 2020, Garcia-Garcia et al., 2017, Chang et al., 2013, Marks and Kalderon, 2011, Zhou and Kalderon, 2011, Ayers et al., 2009, Liu et al., 2007, Zhou et al., 2006)

fu^mh63

(Lefers et al., 2001, Therond et al., 1993)

Name Synonyms

Secondary FlyBase IDs

FBal0004862
FBal0004864

References (27)

Report Sections

Open Close