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General Information
Symbol
Dmel\Rab5UAS.cEa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Entchev
FlyBase ID
FBal0120655
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-rab5
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the Rab5 GM02432 EST.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

egg chamber & basement membrane | apical, with Scer\GAL4slbo.2.6

Detailed Description
Statement
Reference

Expression of Rab5Scer\UAS.cEa under the control of Scer\GAL4ap-md544 in a wild-type background has no effect on leg joint development.

Expression of Rab5Scer\UAS.cEa under the control of Scer\GAL4bbg-C96 appears to have no effect on adult wing formation.

Expression of Rab5Scer\UAS.cEa in border cells, driven by Scer\GAL4slbo.2.6, induces a 5% increase in the number of egg chambers with an apical cap. Micro caps are seen less frequently than in wild-type egg chambers.

Rab5Scer\UAS.cEa, under the regulation of Scer\GAL4elav-C155 does not significantly alter the synaptic area, nor the active zone density of presynaptic terminals, implying that the total number of active zones is normal. The mean quantal content, however, is significantly increased by 1.74 times in Rab5Scer\UAS.cEa mutants compared to wild-type. Because the number of docked vesicles and the spontaneous release rate is normal, the higher quantal content indicates that elevated levels of Rab5 function leads to an increased probability of Ca2+-triggered synaptic vesicle release. Rab5Scer\UAS.cEa; Scer\GAL4elav-C155 larvae have expanded endosomal structures at pre-synaptic termini, producing large tubular structures and multivesicular bodies and only 1-3 endosomal structures per synaptic bouton.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Rab5Scer\UAS.cEa under the control of Scer\GAL4ap-md544 partially suppresses the dsh1 ectopic leg joint phenotype, with partial suppression being seen in 80% of the legs examined.

Expression of egrScer\UAS.cIa and Rab5Scer\UAS.cEa in the eye under the control of Scer\GAL4GMR.PF results in a severe small-eye phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Rab5Scer\UAS.cEa
Rab5UAS.cEa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Entchev
Secondary FlyBase IDs
    References (10)