FlyBase Allele Report: Dmel\hth[100-1]

General Information

Symbol

Dmel\hth^100-1

Species

D. melanogaster

Name

FlyBase ID

FBal0121728

Feature type

allele

Associated gene

Dmel\hth

Associated Insertion(s)

Carried in Construct

Also Known As

hth^100.1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Noro et al., 2006)

Mutagen

ethyl nitrosourea

(Kurant et al., 2001)

Progenitor genotype

Cytology

Description

This allele encodes only the 'HDless' (homeodomainless) isoform and does not encode the 'HM' domain.

(Noro et al., 2006)

Amino acid replacement: R321term.

(Kurant et al., 2001)

Nucleotide substitution: C2213T.

(Kurant et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:10,533,944..10,533,944 [-]

Nucleotide change:

C10533944T

Reported nucleotide change:

C2213T

Amino acid change:

R306term | hth-PA; R321term | hth-PC

Reported amino acid change:

R321term

(Kurant et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | homeotic

(Noro et al., 2006)

Phenotype Manifest In

abdominal segment 1

(Noro et al., 2006)

antenna | somatic clone

(Noro et al., 2006)

embryonic head

(Noro et al., 2006)

embryonic thorax & denticle | ectopic

(Noro et al., 2006)

eye | somatic clone

(Singh et al., 2011)

femur

(Noro et al., 2006)

larval abdominal lch5 neuron

(Kurant et al., 2001)

leg | ectopic | somatic clone

(Noro et al., 2006)

tibia

(Noro et al., 2006)

wing hinge | somatic clone

(Noro et al., 2006)

Detailed Description

Statement

Reference

Loss-of-function hth^100-1 eye clones exhibit a dorsal-ventral constraint in their phenotypes, where clones in the ventral eye result in eye enlargement or induction of ectopic eyes, whereas in the dorsal eye these clones do not affect the eye fate.

(Singh et al., 2011)

hth^100-1 embryos show head defects. In these embryos, A1 is weakly transformed toward A2, but the rest of the abdominal segments have a wild-type pattern of denticle belts. There are no segmental fusions and the thoracic segments are normal except for the rare appearance of a small number of ectopic denticles.

hth^100-1 clones induced in the antennal imaginal disc result in the transformation of the antenna towards a leg. hth^100-1 clones induced in the wing result in aberrant morphology of the wing hinge.

In approximately one-third of hth^100-1 legs the tibia and femur are partially fused.

(Noro et al., 2006)

Mutant embryos show a relatively mild phenotype in the PNS, which consists mainly of dorsal localisation of LCh5 neurons in some of the abdominal segments.

(Kurant et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

hth[+]/hth^100-1 is an enhancer of eye | somatic clone phenotype of L²

(Singh et al., 2011)

hth[+]/hth^100-1 is an enhancer of eye disc | somatic clone phenotype of L²

(Singh et al., 2011)

Suppressor of

Statement

Reference

hth[+]/hth^100-1 is a suppressor of eye | somatic clone phenotype of L^rev6-3

(Singh et al., 2011)

hth[+]/hth^100-1 is a suppressor of eye disc | somatic clone phenotype of L^rev6-3

(Singh et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Loss-of-function L² heterozygous eye clones in a hth^100-1 heterozygous background results in a complete loss-of-ventral eye phenotype, as seen in L² eye clones.

L^rev6-3 heterozygous eye clones in a hth^100-1 heterozygous background results in no ventral eye loss and a wild-type eye phenotype.

(Singh et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

hth^100-1 and hth^100-2 have an identical mutation and are likely to represent a clonal event.

(Kurant et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hth^100-1

(Delker et al., 2019, Kumar et al., 2015, Corsetti and Azpiazu, 2013, Rivas et al., 2013, Singh et al., 2011, Joshi et al., 2010, Noro et al., 2006)

hth^100.1

(Uhl et al., 2016, Li-Kroeger et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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