FlyBase Allele Report: Dmel\EcR[B2.UAS]

General Information

Symbol

Dmel\EcR^B2.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122212

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR.B2}

Also Known As

UAS-EcR-B2, UAS-EcRB2, EcR-B2

Key Links

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

(Lee et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-EcR.B2}

Cytology

Description

UASt sequences drive expression of a 3.13 kb EcoRI cDNA fragment containing the EcR-B2 isoform.

(Lee et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PI

(Cherbas et al., 2003)

partially lethal, with Scer\GAL4^en-e16E

(Schubiger et al., 2003)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Medioni and Noselli, 2005)

polar follicle cell, with Scer\GAL4^slbo.2.6

(Medioni and Noselli, 2005)

wing vein, with Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Detailed Description

Statement

Reference

Expression of EcR^B2.Scer\UAS in the developing wing under the control of Scer\GAL4^Bx-MS1096 results in mild ectopic wing vein formation.

(Kleefstra et al., 2012)

Expression of EcR^B2.Scer\UAS using heat shock at the end of larval development results in adults that have increased total body triglyceride levels (normalised to total body protein) compared to controls.

(Francis et al., 2010)

Expression of EcR^B2.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} does not have an effect on the total amount of sleep either during the day or at night. Average sleep bout duration is also comparable to controls.

(Ishimoto and Kitamoto, 2010)

Expression of EcR^B2.Scer\UAS in border cells, driven by Scer\GAL4^slbo.2.6, causes a border cell cluster migration defect in over 50-60% of egg chambers. However, the apical cap of these egg chambers forms and is shed normally.

(Medioni and Noselli, 2005)

Expression of EcR^B2.Scer\UAS under the control of Scer\GAL4^en-e16E results in some decrease in the hatching rate, but no lethality during metamorphosis; 75% of eggs laid eclose as adults.

(Schubiger et al., 2003)

Expression of EcR^B2.Scer\UAS when driven by Scer\GAL4^Tab2-201Y does not result in any detectable abnormalities.

(Lee et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

EcR^B2.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of G9a^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tab2-201Y/EcR^B2.UAS is a non-suppressor of adult mushroom body gamma-lobe | somatic clone phenotype of babo⁹

(Zheng et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of EcR^B2.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

(Kleefstra et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Scer\GAL4^c42/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Scer\GAL4^MsrA.602/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^Sgs3.PD/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^dpp.blk1/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B2.UAS/Scer\GAL4^slbo.2.6 partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^FMRFa.PS/EcR^B2.UAS partially rescues EcR⁹⁹/EcR³¹

(Schubiger et al., 2003)

Comments

Expressing EcR^B2.UAS under the control of Scer\GAL4^CCAP.PP fully rescues CCAP neuronal death in larvae and the pupal phenotype (failure in head eversion and shortened wings and legs) induced by expression of EcR^{B1-ΔC655.W650A.UAS} alone.

(Lee et al., 2019)

Co-expression of EcR^B2.Scer\UAS results in nearly complete rescue of larval lethality and Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42.

(Gautam et al., 2015)

Expression of EcR^B2.Scer\UAS under the control of Scer\GAL4^ETH.Switch in the presence of RU486 fully rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486.

(Gauthier et al., 2012)

Co-expression of EcR^B2.Scer\UAS rescues the Malpighian tubule defects (reduction in diameter of the tubules and reduction in number and change in distribution of stellate cells) seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of either Scer\GAL4^tsh-c724 or Scer\GAL4^c649.

(Gautam and Tapadia, 2010)

Expression of EcR^B2.Scer\UAS, under the control of Scer\GAL4^Crz restores complete cell death in 87% of EcR³¹/EcR⁹⁹ tissue, while four to eleven neurons are detectable in the remaining specimens.

(Choi et al., 2006)

Co-expression of EcR^B2.Scer\UAS rescues the lethality and eye defects caused by expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of EcR^B2.Scer\UAS rescues to normal viability and normal morphology animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH or Scer\GAL4^Eh.0.8. Co-expression of EcR^B2.Scer\UAS rescues to nearly complete viability animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of EcR^B2.Scer\UAS significantly rescues animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH or Scer\GAL4^Eip71CD.602. Co-expression of EcR^B2.Scer\UAS partially rescues the glue secretion and puffing defects of animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD. Co-expression of EcR^B2.Scer\UAS partially rescues the border cell migration defects, chorion fragility or fertility of females expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6.

(Cherbas et al., 2003)

The failure of pruning of the larval Tv-neurons seen in EcR³¹/EcR⁹⁹ animals is often rescued by expression of EcR^B2.Scer\UAS under the control of Scer\GAL4^Fmrf.PS, with 12 out of 18 nervous systems examined showing complete pruning. "Hyper-pruning" beyond the normal range is also seen in these animals.

(Schubiger et al., 2003)

EcR^B2.Scer\UAS when driven by Scer\GAL4^Tab2-201Y rescues the Mushroom body γ neuron phenotypes.

(Lee et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EcR^B2.Scer\UAS

EcR^B2.UAS

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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