FlyBase Allele Report: Scer\GAL4[tsh-c724]

The expression of tsh in the Malpighian tubules was assayed using the GAL4/UAS system. Reporter gene expression was detected in the stellate cell population of stage 15 embryonic tubules.

(Denholm et al., 2003)

Scer\GAL4^tsh-c724 drives expression in Malpighian tubule Type II cells (stellate cells in the main segment, bar-shaped cells in the in the initial and transitional segments).

(Rosay et al., 1997)

Expressed in the stellate type II cells of the adult and larval Malpighian tubules; also expressed in bar-shaped type II cells in the initial segment of anterior tubules.

(Sozen et al., 1997)

Marker for

Malpighian tubule Type II cell

(Denholm et al., 2013, Söderberg et al., 2011)

Reflects expression of

tsh

(Denholm et al., 2013, Denholm et al., 2003)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal osmotic stress response | adult stage, with SecCl^JF02437

(Feingold et al., 2019)

abnormal starvation stress response | adult stage, with Lkr^KK102546

(Cannell et al., 2016)

decreased body size | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

lethal - all die before end of first instar larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

long lived | conditional, with SecCl^JF02437

(Feingold et al., 2019)

partially lethal, with tsh^JF02856

(Denholm et al., 2013)

partially lethal - majority die, with Myc^UAS.cZa

(Gamberi et al., 2017)

short lived, with tsh^JF02856

(Denholm et al., 2013)

some die during first instar larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

some die during pupal stage, with tsh^JF02856

(Denholm et al., 2013)

viable, with SecCl^JF02437

(Feingold et al., 2019)

visible, with tsh^JF02856

(Denholm et al., 2013)

Phenotype Manifest In

adult abdomen, with tsh^JF02856

(Denholm et al., 2013)

adult Malpighian tubule, with Drip^NIG.9023R

(Cabrero et al., 2020)

adult Malpighian tubule, with Lkr^KK102546

(Cannell et al., 2016)

adult Malpighian tubule, with Myc^UAS.cZa

(Gamberi et al., 2017)

adult Malpighian tubule, with Prip^NIG.7777R

(Cabrero et al., 2020)

adult Malpighian tubule, with tsh^JF02856

(Denholm et al., 2013)

adult Malpighian tubule Type II cell, with tsh^JF02856

(Denholm et al., 2013)

embryonic/larval Malpighian tubule | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

embryonic/larval Malpighian tubule Type II cell, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

Malpighian tubule, with 5-HT7^UAS.cKa

(Kerr et al., 2004)

Malpighian tubule, with Besp\bPAC^UAS.cSa, Scer\GAL4^c42

(Efetova et al., 2013)

Malpighian tubule | conditional, with Besp\bPAC^UAS.cSa

(Efetova et al., 2013)

Malpighian tubule | drug conditional, with Rnor\Npr1^UAS.cKa

(Kerr et al., 2004)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^tsh-c724, tsh^JF02856 has partially lethal phenotype, suppressible | partially by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Scer\GAL4^tsh-c724, tsh^JF02856 has visible phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Suppressor of

Statement

Reference

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor | partially of partially lethal phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of visible phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724 has Malpighian tubule | conditional phenotype, enhanceable by Epac^UAS.cEa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^tsh-c724, tsh^JF02856 has adult Malpighian tubule Type II cell phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Scer\GAL4^tsh-c724, tsh^JF02856 has adult abdomen phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724 has Malpighian tubule | conditional phenotype, suppressible by Pka-C1^RNAi.UAS.cUa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Enhancer of

Statement

Reference

Epac^UAS.cEa, Scer\GAL4^tsh-c724 is an enhancer of Malpighian tubule | conditional phenotype of Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Suppressor of

Statement

Reference

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule Type II cell phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of adult abdomen phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

Pka-C1^RNAi.UAS.cUa, Scer\GAL4^tsh-c724 is a suppressor of Malpighian tubule | conditional phenotype of Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^tsh-c724/EcR^B2.UAS rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam and Tapadia, 2010)

Fails to rescue

SecCl^UAS.cFa/Scer\GAL4^tsh-c724 fails to rescue SecCl^G6893

(Feingold et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

99567

P{GawB}tsh^c724

86330

P{GawB}tsh^c724; P{tubP-GAL80^ts}ncd^GAL80ts-7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^c724

Scer\GAL4^c724

Scer\GAL4^tsh-c724

Name Synonyms

Secondary FlyBase IDs

References (23)

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