FlyBase Insertion Report: Dmel\P{GawB}tsh[c724]

General Information

Symbol

Dmel\P{GawB}tsh^c724

Species

D. melanogaster

Name

FlyBase ID

FBti0207637

Feature type

transposable_element_insertion_site

Also Known As

c724-Gal4, c724, P{GAL4}C724

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, tsh

Viability / fertility

viable

Associated allele(s)

Scer\GAL4^tsh-c724, tsh^c724

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2L ( 40C )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

40C (in situ hybridization reported)

(Sozen et al., 1997)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(FlyBase, 1992-)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Cabrero et al., 2013, Gautam and Tapadia, 2010, Johnson et al., 2005, Broderick et al., 2004, Kerr et al., 2004, Denholm et al., 2003, Rosay et al., 1997, Sozen et al., 1997)

tsh

(Denholm et al., 2003)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^tsh-c724

Phenotypic Class Phenotype Manifest in Detailed Description

tsh^c724

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

Malpighian tubule Type II cell

(Sozen et al., 1997)

adult stage

Malpighian tubule Type II cell

(Sozen et al., 1997)

initial segment of Malpighian tubule | restricted

(Sozen et al., 1997)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

embryonic Malpighian tubule | restricted

(Denholm et al., 2003)

embryonic stage 17

embryonic/larval Malpighian tubule Type II cell

(Gautam and Tapadia, 2010)

embryonic/larval salivary gland

(Gautam and Tapadia, 2010)

larval stage

Malpighian tubule Type II cell

(Sozen et al., 1997)

first instar larval stage

embryonic/larval Malpighian tubule Type II cell

(Gautam and Tapadia, 2010)

embryonic/larval salivary gland

(Gautam and Tapadia, 2010)

larval visceral muscle cell

(Gautam and Tapadia, 2010)

adult stage

initial segment of Malpighian tubule

(Sozen et al., 1997)

Malpighian tubule Type II cell

(Sozen et al., 1997)

adult Malpighian tubule stellate cell of main segment

(Xu et al., 2023, Cabrero et al., 2020)

adult Malpighian tubule Type II cell

(Rosay et al., 1997)

adult Malpighian tubule bar-shaped cell

(Rosay et al., 1997)

Additional Information

Statement

Reference

Drives expression in Malpighian tubule type II cells and stellate cells.

(FlyBase Curators, 2017)

The expression of tsh in the Malpighian tubules was assayed using the GAL4/UAS system. Reporter gene expression was detected in the stellate cell population of stage 15 embryonic tubules.

(Denholm et al., 2003)

Scer\GAL4^tsh-c724 drives expression in Malpighian tubule Type II cells (stellate cells in the main segment, bar-shaped cells in the in the initial and transitional segments).

(Rosay et al., 1997)

Expressed in the stellate type II cells of the adult and larval Malpighian tubules; also expressed in bar-shaped type II cells in the initial segment of anterior tubules.

(Sozen et al., 1997)

Marker for

Malpighian tubule Type II cell

(Denholm et al., 2013, Söderberg et al., 2011)

Reflects expression of

tsh

(Denholm et al., 2013, Denholm et al., 2003)

Reporter construct used in assay

Avic\aeq^UAS.cRa

(Rosay et al., 1997)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

99567

P{GawB}tsh^c724

86330

P{GawB}tsh^c724; P{tubP-GAL80^ts}ncd^GAL80ts-7

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

C724-Gal4

(Weavers and Skaer, 2013, Söderberg et al., 2011)

P{GAL4}^c724

(Johnson et al., 2005)

P{GAL4}C724

(Denholm et al., 2003, FlyBase, 1992-)

P{GAL4}c724

(Sozen et al., 1997, FlyBase, 1992-)

P{GawB}c724

(Sozen et al., 1997, FlyBase, 1992-)

P{GawB}tsh^c724

(FlyBase, 1992-)

c724

(Gamberi et al., 2017, Cabrero et al., 2014, Cabrero et al., 2013, Gautam and Tapadia, 2010, Broderick et al., 2004)

c724-GAL4

(Xu et al., 2023, Cabrero et al., 2020, Cannell et al., 2016)

c724-Gal4

(Ceder et al., 2020, Feingold et al., 2019, Highfill et al., 2017, Denholm et al., 2013, Efetova et al., 2013)

c724: GAL4

(Kerr et al., 2004)

c724::GAL4

(Johnson et al., 2005)

tsh(c724)-Gal4

(Okamoto et al., 2026)

tsh-GAL4

(Xu et al., 2024)

tshGal4

(Denholm et al., 2003)

{GAL4}C724

(Denholm et al., 2003)

Name Synonyms

Secondary FlyBase IDs

FBti0004375
FBti0009566
FBti0037984

References (23)

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