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General Information
Symbol
Dmel\par-1N1S.UASp.mGFP6
Species
D. melanogaster
Name
FlyBase ID
FBal0122938
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GFP-PAR-1 N1S, Par1-GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of the N1S isoform of par-1 tagged at the N-terminal end with mGFP6.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

par-1N1S.UASp.mGFP6 is localized on the plasma membrane and enriched at the posterior of the oocyte in control egg chambers .

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The oocyte nucleus is often found mislocalised to the centre of the oocyte in stag 9-10 egg chambers of females expressing two copies of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16. Expression of one copy of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 has no effect on oocyte nucleus localisation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The oocyte nucleus is mislocalised to the centre of the oocyte in 43% of egg chambers of females co-expressing par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 and l(2)gl3A.Scer\UAS.P\T under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

Xenogenetic Interactions
Statement
Reference

The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6.

Complementation and Rescue Data
Comments

Expression of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 fully rescues the posterior localisation of osk mRNA in the egg chamber.

par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 rescues the posterior movement of the centrosomes and the maintenance of oocyte identity when expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in par-1W3 germline clones.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6
par-1N1S.Scer\UAS.T:Avic\GFP-m6
par-1N1S.UASp.mGFP6
Name Synonyms
Secondary FlyBase IDs
    References (16)