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General Information
Symbol
Dmel\sliGA20
Species
D. melanogaster
Name
FlyBase ID
FBal0123697
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
slitGA20
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Amino acid change:

L837term | sli-PC, L813term | sli-PB, L813term | sli-PA

Reported amino acid change:

L?term

Comment:

A one base deletion leads to early translation termination.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of one base in G-Domain results in a frameshift and immediate translation termination.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In stage 16 sliGA20 mutant embryos 46% of the ganglionic branches cross the midline and the rest are either stalled outside the ventral nerve cord or misrouted inside it.

1.9% of expected mutant embryos hatch as first instar larvae. The latest observed stage of mutants is L1. Nerve cord length is 90% of embryo length. Midline guidance failure of repellence phenotype, evidenced by midline crossing, is severe.

The GMC-1 in mutants frequently (about 10%) divide symmetrically to generate two RP2s instead of an RP2 and a sib. The RP2 and aCC pCC and other neurons are occasionally misplaced within a hemisegment of the CNS of 13-hour or older mutant embryos, however in younger embryos these cells are not displaced and do not cross the midline or segmental boundary.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

sliGA20 has larval ventral nerve cord phenotype, enhanceable by robo[+]/robo11

Additional Comments
Genetic Interactions
Statement
Reference

23.8% of dMP2 neurons project contralaterally in sliGA20/robo1 transheterozygous embryos. This phenotype is suppressed by expression of sliScer\UAS.cKa under the control of Scer\GAL4605.

The effects of sliGA20 and ruPLLb on migration of ganglionic branch trachea approaching the ventral midline during stage 16 appear to be additive.

Percentage of segments with midline crossovers in robo1/sliGA20 transheterozygotes is 60%.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Protein null.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)