FlyBase Allele Report: Dmel\snf[148]

General Information

Symbol

Dmel\snf¹⁴⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0124934

Feature type

allele

Associated gene

Dmel\snf

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Swan et al., 2001)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of germ cell cancer

CEA

(Chau et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of germ cell cancer

is ameliorated by CycB²

(Chau et al., 2009)

is ameliorated by Sxl^cF1.otu

(Chau et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Gawande et al., 2006, Nagengast et al., 2003, Swan et al., 2001)

neoplasia | dominant | oogenesis

(Primus et al., 2019)

suppressor of variegation, with P{SUPor-P}KV00108

(Swenson et al., 2016)

Phenotype Manifest In

egg chamber

(Nagengast et al., 2003)

female germline cell

(Mercer et al., 2025)

germarium

(Primus et al., 2019, Chau et al., 2012, Swan et al., 2001)

ovariole

(Mercer et al., 2025)

ovary

(Primus et al., 2019, Chau et al., 2009)

Detailed Description

Statement

Reference

snf¹⁴⁸ leads to a dominant 'germline tumor' phenotype, which is mild and rare in heterozygotes, while strong and fully prevalent in homozygotes.

(Primus et al., 2019)

snf¹⁴⁸/snf¹⁴⁸ suppresses position effect variegation at the w locus caused by P{SUPor-P}KV00108.

(Swenson et al., 2016)

Homozygous females have an ovarian tumour phenotype, and tumour growth continues through adulthood.

(Chau et al., 2012)

Larval snf¹⁴⁸ ovaries do not contain an excess of germ cells and a tumorous phenotype is not apparent in ovaries from animals at the larval/pupal transition.

The ovarian tumour of snf¹⁴⁸ females is apparent in newborn adults, and increase in size as the fly ages. As the tumour grows, the somatic gonadal cells surround some of the tumour cells to form a structure that resembles an egg chamber. Dividing cells in snf¹⁴⁸ adult ovaries are not confined to the anterior half of the germarium (as occurs in wild-type females) and are seen throughout the ovary, even in pseudo-egg chambers. The mutant tumours are composed of both single cells with round spectrosomes and cells with abnormal fusome-like structures. Both single and interconnected germ cells are observed to be in mitosis in the mutant ovaries, irrespective of their position within the germarium. Analysis using markers indicates that the majority of the tumour cells retain characteristics of both stem and their differentiating daughter cells.

(Chau et al., 2009)

Mutant egg chambers are filled with many small nuclei, and ovarian tumor phenotype.

(Nagengast et al., 2003)

Mutation results in a tumourous germarium.

(Swan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

snf¹⁴⁸ has neoplasia | oogenesis | dominant phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

Suppressed by

Statement

Reference

snf¹⁴⁸ has decreased cell number | oogenesis phenotype, suppressible by Sxl^cF1.otu

(Mercer et al., 2025)

snf¹⁴⁸ has female sterile phenotype, suppressible by Sxl^cF1.otu

(Gawande et al., 2006, Nagengast et al., 2003)

Other

Statement

Reference

Sxl^cF1.otu, Sxl^fP7B0, snf¹⁴⁸ has lethal phenotype

(Nagengast et al., 2003)

Sxl^fP7B0/Sxl^cF1.otu, snf¹⁴⁸ has lethal phenotype

(Nagengast et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

snf¹⁴⁸ has ovariole phenotype, enhanceable by bbn^KO

(Mercer et al., 2025)

snf¹⁴⁸ has germarium phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

snf¹⁴⁸ has ovary phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

Suppressed by

Statement

Reference

snf¹⁴⁸ has ovariole phenotype, suppressible by Sxl^cF1.otu

(Mercer et al., 2025)

snf¹⁴⁸ has female germline cell phenotype, suppressible by Sxl^cF1.otu

(Mercer et al., 2025)

Enhancer of

Statement

Reference

snf¹⁴⁸ is an enhancer of ovariole phenotype of bbn^KO

(Mercer et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Tumour growth is strongly suppressed in the ovaries of snf¹⁴⁸/snf¹⁴⁸ ; nos¹⁸/Df(3R)Exel6183 females. In young animals, 35% of germaria are agametic, In older animals the fraction of agametic germaria increases to 74%, with a marked reduction in tumour size compared with similar aged snf¹⁴⁸/snf¹⁴⁸ females. The majority of surviving double mutant germ cells have abnormal fusome-like structures and fail to differentiate (indicating that they are more similar to snf than nos mutant germ cells).

(Chau et al., 2012)

snf¹⁴⁸ ; CycB² double mutant ovaries are not tumourous and contain few or no germ cells, similar to the CycB² single mutant phenotype.

snf¹⁴⁸ ; zpg^z-2533 double mutant ovaries are not tumourous and an average of only 11 +/- 0.4 germline cells per germarium, similar to the zpg^z-2533 single mutant phenotype.

The fusome phenotype of snf¹⁴⁸ ; bam^Δ86 double mutant ovaries is more similar to that of the snf¹⁴⁸ single mutant phenotype than that of the bam^Δ86 single mutant phenotype.

(Chau et al., 2009)

The female progeny of snf¹⁴⁸/+ females mated to Sxl^fP7B0/Y males show only 48.0% viability.

The female progeny of snf¹⁴⁸/+ ; fl(2)d¹/+ females mated to Sxl^fP7B0/Y males show only 11.1% viability.

The female progeny of snf¹⁴⁸/+ ; fl(2)d²/+ females mated to Sxl^fP7B0/Y males show only 45.6% viability.

(Penn et al., 2008)

Expression of Sxl^cF1.otu restores fertility in snf¹⁴⁸ female flies.

(Gawande et al., 2006)

snf¹⁴⁸ has no significant effect on the survival rate of snRNP70K^ON35/snRNP70K² ; snRNP70K::Hsap\SFRS1^HCterm adults. snf¹⁴⁸ has no significant effect on the survival rate of snRNP70K^ON35/snRNP70K² ; snRNP70K^+t9 adults. snf¹⁴⁸/Y ; snRNP70K^ON35/snRNP70K² ; snRNP70K::Hsap\SFRS1^{ΔRrich-HCterm} adults are never recovered.

(Salz et al., 2004)

Homozygous snf¹⁴⁸ females are sterile. If their sterility is rescued by Sxl^cF1.otu, the resulting males and females have equivalent wild-type survival rates. If snf¹⁴⁸/snf¹⁴⁸, Sxl^cF1.otu females are crossed with Sxl^fP7B0 males only 6% of her Sxl^fP7B0/+ daughters survive.

(Nagengast et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7398

y¹ w¹¹¹⁸ snf¹⁴⁸ P{neoFRT}19A/FM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Snf¹⁴⁸

(Primus et al., 2019)

snf¹⁴⁸

(Mercer et al., 2025, Hudry et al., 2019, Swenson et al., 2016, Li et al., 2013, Chau et al., 2012, Tastan et al., 2010, Chau et al., 2009, Penn et al., 2008, Gawande et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (13)

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