FlyBase Allele Report: Dmel\kay[N-Ala.UAS]

General Information

Symbol

Dmel\kay^N-Ala.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0125003

Feature type

allele

Associated gene

Dmel\kay

Associated Insertion(s)

Carried in Construct

P{UAS-D-fos^N-Ala}

Key Links

Transgenic product class

missense_variant

(Ciapponi et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ciapponi et al., 2001)

Mutagen

in vitro construct

(Ciapponi et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-D-fos^N-Ala}

Cytology

Description

UASt drives expression of a mutated kay cDNA (carries the amino acid replacements T89A and T93A).

(Ciapponi et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kay

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, scrib¹

(Külshammer et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing, with Scer\GAL4^en-e16E

(Bosch et al., 2005)

Phenotype Manifest In

thorax, with Scer\GAL4^69B

(Ciapponi et al., 2001)

Detailed Description

Statement

Reference

Only 35% of wing discs with wounds in the posterior compartment show normal wound healing when kay^{N-Ala.Scer\UAS} (a mutant that cannot be phosphorylated by the JNK pathway) is overexpressed under the control of Scer\GAL4^en-e16E.

(Bosch et al., 2005)

kay^{N-Ala.Scer\UAS} when driven by Scer\GAL4^69B, gives rise to a thoracic cleft at the dorsal midline. When kay^{N-Ala.Scer\UAS} is driven by Scer\GAL4^en-e16E, no effect is seen on wing vein development.

(Ciapponi et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

kay^N-Ala.UAS, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of majority die during third instar larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

kay^N-Ala.UAS, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of abnormal developmental rate | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

kay^N-Ala.UAS, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Scer\GAL4^hs.2sev/kay^N-Ala.UAS is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Ciapponi et al., 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

kay^N-Ala.UAS, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

kay^N-Ala.UAS, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of photoreceptor neuron | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Scer\GAL4^hs.2sev/kay^N-Ala.UAS is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Ciapponi et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^unspecified/kay^N-Ala.UAS is a non-suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Ciapponi et al., 2001)

Scer\GAL4^unspecified/kay^N-Ala.UAS is a non-suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Ciapponi et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Clonal co-expression of kay^{N-Ala.Scer\UAS} partially suppresses the lethality and developmental delay seen in larvae with scrib¹ mutant eye-antennal disc clones expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU, and suppresses the invasive infiltration of the ventral nerve cord exhibited by cells of these clones, although the tumor mass is not significantly decreased.

Clonal co-expression of kay^{N-Ala.Scer\UAS} partially suppresses the defective photoreceptor differentiation seen in scrib¹ mutant eye-antennal disc clones expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU.

(Külshammer et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kay^{N-Ala.Scer\UAS}

kay^N-Ala.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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