FlyBase Allele Report: Dmel\RhoGAPp190[RNAi.N.UAS]

General Information

Symbol

Dmel\RhoGAPp190^RNAi.N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0128099

Feature type

allele

Associated gene

Dmel\RhoGAPp190

Associated Insertion(s)

Carried in Construct

P{UAS-RhoGAPp190.N.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Billuart et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Billuart et al., 2001)

Mutagen

in vitro construct

(Billuart et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-RhoGAPp190.N.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of two copies of RhoGAPp190 sequence (the N-terminal domain), arranged in an inverted repeat.

(Billuart et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

RhoGAPp190

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by TBPH^Δ23, TBPH^GD6943

(Langellotti et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Jeong et al., 2012)

Phenotype Manifest In

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^elav.PLu

(Jeong et al., 2012)

vertical lobe of adult mushroom body, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

Detailed Description

Statement

Reference

Expression of RhoGAPp190^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^elav.PLu results in premature defasciculation of ISNb axons prior to reaching muscle 13, and sometimes muscle 6, reflecting either increased defasciculation or a defect in muscle target recognition. The ISNb premature branching phenotype is seen in 22.1% of hemisegments, while the total fraction of hemisegments showing ISNb defects is 36.4%.

(Jeong et al., 2012)

Expression of RhoGAPp190^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^OK107 results in truncation or loss of dorsal branches of the mushroom body.

(Billuart et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(4)C3/+

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by PlexB^KG00878/plexB[+]

(Jeong et al., 2012)

Suppressed by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by pbl[+]/pbl²

(Jeong et al., 2012)

NOT suppressed by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Df(4)C3/+

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by PlexB^KG00878/plexB[+]

(Jeong et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-enhanceable by Df(4)C3/+

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-enhanceable by PlexB^KG00878/plexB[+]

(Jeong et al., 2012)

Suppressed by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, suppressible | partially by pbl[+]/pbl²

(Jeong et al., 2012)

NOT suppressed by

Statement

Reference

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-suppressible by Df(4)C3/+

(Jeong et al., 2012)

RhoGAPp190^RNAi.N.UAS, Scer\GAL4^elav.PLu has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-suppressible by PlexB^KG00878/plexB[+]

(Jeong et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Sema-1a^k13702/+ suppresses the premature ISNb branching seen in embryos expressing RhoGAPp190^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^elav.PLu from 22.1% to 8.2% of hemisegments, while the total fraction of hemisegments showing ISNb defects is reduced from 36.4% to 21.2% in these animals.

Heterozygosity for either Df(4)C3 or plexB^KG00878 has no effect on the mutant ISNb phenotype seen in embryos expressing RhoGAPp190^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^elav.PLu.

pbl²/+ significantly reduces the penetrance of the ISNb premature branching phenotype seen in embryos expressing RhoGAPp190^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^elav.PLu (from 22.1% to 9.4% of hemisegments). These embryos show a significant increase in defasciculation defects (excluding premature branching defects) at the last ISNb choice point.

(Jeong et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6429

y¹ w^*; P{UAS-RhoGAPp190.N.RNAi}8.2

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-RhoGAPp190.N-dsRNA} construct should produce RhoGAPp190 dsRNA, resulting in dsRNA interference (RNAi) of the RhoGAPp190 gene.

(Billuart et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RhoGAPp190^RNAi.N.UAS

RhoGAPp190^{dsRNA.N.Scer\UAS}

RhoGAPp190^dsRNA.N.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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