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General Information
Symbol
Dmel\TBPHGD6943
Species
D. melanogaster
Name
FlyBase ID
FBal0208690
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
TBPHRNAi, TBPH-RNAi, UAS-TBPH RNAi, UAS-dTDP-43 RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of TBPHGD6943 under the control of Scer\GAL4GMR.PS leads to no obvious eye phenotype, as compared to controls.

Expression of TBPHGD6943 RNAi under the control of Scer\GAL4Toll-6-D42 lengthens the time it takes for third instar larvae to roll over in a turning assay.

Expression of TBPHGD6943 under the control of Scer\GAL4repo.PU (in combination with Dcr-2 expression for efficient RNAi) in a TBPHΔ23 heterozygous background results in strong motility defects, as well as consistent reduction of life span, compared with wild-type

Flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PU in a TBPHΔ23/+ mutant background exhibit significant climbing defects, a severe reduction in life span, and degeneration of the neuropil, as compared to controls.

Flies expressing TBPHGD6943 in a TBPHΔ23/+ mutant background under the control of Scer\GAL4tub.PU, and restricted to the adult stage using Scer\GAL80ts.αTub84B, exhibit progressive climbing defects and reduced lifespan.

Larvae expressing TBPHGD6943 under the control of Scer\GAL4tub.PU from the third instar larval stage onward (using Scer\GAL80ts.αTub84B) in a TBPHΔ23/+ background) exhibit locomotor defects from 24 hours after the temperature shift. These alterations in fly motility are not associated with anatomical modifications in the number of synaptic boutons or motoneuron terminal branches at the NMJ, except for the presence of subtle alterations in the morphology of the synaptic boutons compared to controls.

Expression of TBPHGD6943 under the control of Scer\GAL4Act.PU results in a subset of adults possessing supernumerary scutellar bristles or supernumerary anterior postalar bristles.

Expression of TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 results in locomotor defects in third instar larvae. Crawling larvae rolled ventral side up take longer to turn back to dorsal side up.

Adults expressing TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 exhibit a progressive decline in their ability to climb at 18[o]C, and exhibit a decrease in lifespan, as compared to controls.

When TBPHGD6943 is expressed pan-neuronally under the control of Scer\GAL4elav.PU the synaptic boutons on muscles 6 and 7 in third instar larvae appear deformed and are irregularly spaced along the terminal axons with several fused or elongated silhouettes and clear loss of their characteristic round-smooth shape.

Expression of TBPHGD6943 under the control of Scer\GAL4ey-OK107 does not affect the morphology of the mushroom body lobes. The flies show a small (10%) but significant reduction in performance in an odour avoidance learning assay compared to controls.

Expression of TBPHGD6943 under the control of either Scer\GAL4Mhc.PU or Scer\GAL4D42 does not result in locomotor defects.

Expression of TBPHGD6943 under the control of Scer\GAL4elav.PU results in locomotor defects in larvae. The number of synaptic boutons normalised to the muscle area is increased at the neuromuscular junction in these animals.

No obvious eye defect is found in flies expressing TBPHGD6943 under the control of Scer\GAL4GMR.PF.

Expression of TBPHGD6943 in the mushroom body (MB) under the control of Scer\GAL4ey-OK107 results in axonal loss and neuronal death. No obvious abnormal axonal varicosities are detected in TBPHGD6943-expressing MBs.

The locomotive ability of larvae expressing TBPHGD6943 under the control of Scer\GAL4VGlut-OK371 is normal.

Adults expressing TBPHGD6943 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of TBPHGD6943 in the developing eye under the control of Scer\GAL4GMR.PF does not affect eye development.

Adults expressing TBPHGD6943 under the control of either Scer\GAL41407 or Scer\GAL4elav.PLu show defects in climbing ability.

Flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PLu in a TBPHΔ23/+ background show defects in both walking and climbing assays.

Expression of TBPHGD6943 under the control of Scer\GAL4221 in a TBPHQ367X/+ background results in a significant decrease in dendritic branching in the ddaE and ddaF neurons of third instar larvae.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The longer time it takes for third instar larvae expressing TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 to roll over in a turning assay compared to wild-type controls is lengthened further by co-expression of Fmr1GD1288 RNAi.

Knockdown of TBPH, through expression of TBPHGD6943, enhances the eye phenotype seen in flies expressing Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU.

Co-expression of Hrb98DEJF01249 enhances the climbing defects, shortened life span and neuropil degeneration seen in TBPHΔ23/+ flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PU.

Expression of TBPHGD6943 under the control of Scer\GAL4elav.PU enhances the climbing defects, shortened life span and neuropil degeneration seen in TBPHΔ23/+ flies expressing Hrb98DEJF01249 under the control of Scer\GAL4elav.PU.

Expression of Syx1AScer\UAS.cBa significantly suppresses the climbing defects seen in adult hypomorphic TBPH mutants (generated by expressing TBPHGD6943 in adult flies under the control of Scer\GAL4tub.PU and Scer\GAL80ts.αTub84B, in a TBPHΔ23/+ background).

Expression of TBPHGD6943 in the developing eye dominantly suppresses the eye degeneration phenotype found upon expression of TER94R152H.Scer\UAS.R (with both transgenes driven by Scer\GAL4GMR.PF). This is concurrent with a significant reduction in the blinded phenotypic severity score.

Xenogenetic Interactions
Statement
Reference

The co-expression of TBPHGD6943 enhances the severity of the eye defects resulting from the expression of Zzzz\UGGAAW.Scer\UAS under the control of Scer\GAL4GMR.PS (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities); the severe eye defects resulting from this co-expression are fully or partially suppressed by the additional co-expression of Hsap\FUSScer\UAS.cIa or Hsap\HNRNPA2B1Scer\UAS.cKa, respectively.

The co-expression of TBPHGD6943 and Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS leads to lethality, which is rescued by the additional co-expression of either Hsap\FUSScer\UAS.cIa or Hsap\HNRNPA2B1Scer\UAS.cKa; the rescued adults exhibit significantly less severe eye phenotypes, as compared to adults only expressing Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS.

Expression of TBPHGD6943 enhances the third instar larval locomotor defects seen when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed in motor neurons under the control of Scer\GAL4D42.

Expression of TBPHGD6943 enhances the third instar larval locomotor defects seen when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed in motor neurons under the control of Scer\GAL4D42.

Co-expression of TBPHGD6943 with Hsap\TARDBPScer\UAS.T:Ivir\haemagglutinin,T:Disc\RFP under the control of Scer\GAL4VGlut-OK371 results in improved larval movement compared to the Hsap\TARDBPScer\UAS.T:Ivir\haemagglutinin,T:Disc\RFP-expressing parental line.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (23)