FlyBase Allele Report: Dmel\unc-5[UAS.cKa]

General Information

Symbol

Dmel\unc-5^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Keleman

FlyBase ID

FBal0128973

Feature type

allele

Associated gene

Dmel\unc-5

Associated Insertion(s)

Carried in Construct

P{UAS-unc-5.K}

Also Known As

UAS-Unc5, UAS-unc-5

Key Links

Transgenic product class

wild_type

(Keleman and Dickson, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Keleman and Dickson, 2001)

Mutagen

in vitro construct

(Keleman and Dickson, 2001)

Progenitor genotype

Carried in construct

P{UAS-unc-5.K}

Cytology

Description

Expression of full length unc-5 is governed by UASt regulatory sequences.

(Keleman and Dickson, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

unc-5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell body & serotonin neuron, with Scer\GAL4^eg-Mz360

(Couch et al., 2004)

embryonic longitudinal glial cell, with Scer\GAL4^gcm-rA87.P

(Freeman et al., 2003)

larval anterior commissure, with Scer\GAL4^elav.PLu

(Keleman and Dickson, 2001)

larval longitudinal connective, with Scer\GAL4^NP6558

(Brankatschk and Dickson, 2006)

larval posterior commissure, with Scer\GAL4^elav.PLu

(Keleman and Dickson, 2001)

larval ventral nerve cord commissure, with Scer\GAL4^insc-Mz1407

(Brankatschk and Dickson, 2006)

photoreceptor cell R7, with Scer\GAL4^GMR.PF

(Berger-Müller et al., 2013)

presumptive embryonic salivary gland, with Scer\GAL4^prd.RG1

(Kolesnikov and Beckendorf, 2005)

serotonin neuron & ventral nerve cord, with Scer\GAL4^eg-Mz360

(Couch et al., 2004)

Detailed Description

Statement

Reference

R7 photoreceptor axons that over-express unc-5^Scer\UAS.cKa (under the control of Scer\GAL4^GMR.PF) are redirected to the more superficial layer M0-1.

(Berger-Müller et al., 2013)

Misexpression of unc-5^Scer\UAS.cKa throughout the PNS under the control of Scer\GAL4^5-40 results in disruption of sensory input patterns. Whie the sensory axons project normally within the peripheral nerves to the CNS, once they arrive they fail to grow towards the midline and tend to stall laterally, or in some cases fail to enter the CNS altogether. Despite the disruption of the sensory input, the overall structure of the CNS appears normal, although subtle secondary defects would not be detectable. Expression of unc-5^Scer\UAS.cKa phenocopies the effects of inhibiting the neurons and results in propagation defects in the crawling pattern.

(Hughes and Thomas, 2007)

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷.

The axons of sensory neurons that express Scer\GAL4^ato-NP6558 normally arborise in the intermediate region of the longitudinal connective when they enter the central nervous system (CNS). When these axons are forced to express unc-5^Scer\UAS.cKa via Scer\GAL4^ato-NP6558, they terminate instead lateral to the connective.

(Brankatschk and Dickson, 2006)

When unc-5^Scer\UAS.cKa is expressed in embryonic salivary glands, driven by Scer\GAL4^prd.RG1, the glands become oriented laterally away from the CNS midline, instead of lying parallel to it as in wild type. This phenotype has 70% penetrance.

(Kolesnikov and Beckendorf, 2005)

In the ventral nerve cord of stage 16 unc-5^Scer\UAS.cKa; Scer\GAL4^eg-Mz360 embryo, serotonergic neurons fail to cross the midline. In addition, as subset of these neurons lose their serotonin uptake ability. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 unc-5^Scer\UAS.cKa; Scer\GAL4^eg-Mz360 embryos are shifted laterally compared to wild-type.

(Couch et al., 2004)

Embryos expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4^gcm.PP show a block in the medial migration of longitudinal glia and an excess of glia migrating to the lateral edge of the central nervous system and onto the nerve roots.

(Freeman et al., 2003)

When expression is driven by Scer\GAL4^elav.PLu commissures are completely lacking from the embryonic CNS, because the commissural axons are prevented from crossing the midline.

(Keleman and Dickson, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, suppressible by Df(1)NetAB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, suppressible by Df(1)NetAB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, suppressible by NetB^tm/NetA^Δ

(Brankatschk and Dickson, 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP6558, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetB^tm/NetA^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetA^Δ/NetB^myc

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetA^Δ/NetB^myc

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetA^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has abnormal neuroanatomy phenotype, non-suppressible by NetA^Δ

(Brankatschk and Dickson, 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has larval ventral nerve cord commissure phenotype, suppressible by Df(1)NetAB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has larval longitudinal connective phenotype, suppressible by Df(1)NetAB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has larval longitudinal connective phenotype, suppressible by NetB^tm/NetA^Δ

(Brankatschk and Dickson, 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has larval ventral nerve cord commissure phenotype, non-suppressible by NetA^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has larval ventral nerve cord commissure phenotype, non-suppressible by NetB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has larval longitudinal connective phenotype, non-suppressible by NetA^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has larval longitudinal connective phenotype, non-suppressible by NetB^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has larval ventral nerve cord commissure phenotype, non-suppressible by NetB^tm/NetA^Δ

(Brankatschk and Dickson, 2006)

Scer\GAL4^insc-Mz1407, unc-5^UAS.cKa has larval ventral nerve cord commissure phenotype, non-suppressible by NetA^Δ/NetB^myc

(Brankatschk and Dickson, 2006)

Scer\GAL4^NP6558, unc-5^UAS.cKa has larval longitudinal connective phenotype, non-suppressible by NetA^Δ/NetB^myc

(Brankatschk and Dickson, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷ in a homozygous NetA^Δ mutant genetic background.

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷ in a homozygous NetB^Δ mutant genetic background.

Both ventral nerve cord commissures are present in the majority of segments of embryos expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷ in a homozygous Df(1)NetAB^Δ mutant genetic background.

Homozygous NetA^Δ has little effect on long-range repulsion of axons expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4^ato-NP6558.

Homozygous NetB^Δ has little effect on long-range repulsion of axons expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4^ato-NP6558.

Homozygous Df(1)NetAB^Δ completely eliminates the long-range repulsion of axons expressing unc-5^Scer\UAS.cKa under the control of Scer\GAL4^ato-NP6558.

Short-range repulsion is preserved in NetA^Δ, NetB^{KI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC} embryos that express unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷, as the ventral nerve cord commissures are completely absent in every segment examined.

Short-range repulsion is preserved in NetA^Δ, NetB^{KI.T:Hsap\MYC} embryos that express unc-5^Scer\UAS.cKa under the control of Scer\GAL4¹⁴⁰⁷, as the ventral nerve cord commissures are completely absent in every segment examined.

Long-range axon-repulsion is completely abolished in NetA^Δ, NetB^{KI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC} embryos that express unc-5^Scer\UAS.cKa under the control of Scer\GAL4^ato-NP6558.

Long-range axon-repulsion is preserved in NetA^Δ, NetB^{KI.T:Hsap\MYC} embryos that express unc-5^Scer\UAS.cKa under the control of Scer\GAL4^ato-NP6558.

(Brankatschk and Dickson, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Five out of five inserts tested gave completely penetrant phenotype with single copy of construct.

(Keleman and Dickson, 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

unc-5^Scer\UAS.cKa

unc-5^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Keleman

(Keleman and Dickson, 2001)

Secondary FlyBase IDs

References (7)

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