FlyBase Allele Report: Dmel\PGRP-SA[seml]

General Information

Symbol

Dmel\PGRP-SA^seml

Species

D. melanogaster

Name

FlyBase ID

FBal0130837

Feature type

allele

Associated gene

Dmel\PGRP-SA

Associated Insertion(s)

Carried in Construct

Also Known As

seml

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Michel et al., 2001)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: C80Y. Nucleotide substitution: G174A. The mutation is within the PGRP domain.

(Michel et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,562,211..11,562,211 [+]

Nucleotide change:

G11562211A

Reported nucleotide change:

G174A

Amino acid change:

C80Y | PGRP-SA-PA; C80Y | PGRP-SA-PB

Reported amino acid change:

C80Y

(Michel et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Geuking et al., 2009, Wang et al., 2008, Bischoff et al., 2004, Pili-Floury et al., 2004, Gobert et al., 2003, Michel et al., 2001)

abnormal immune response | adult stage

(Iatsenko et al., 2016, Reed et al., 2015, Garver et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

PGRP-SA^seml mutant flies show reduced survival rate upon infection with either Staphylococcus aureus or Streptococcus pyogenes compared to wild-type.

(Iatsenko et al., 2016)

PGRP-SA^seml mutant flies show significantly decreased survival rate upon infection with the Staphylococcus aureus strain COL and unlike wild-type flies is strongly susceptible even to infection with the strain COL MIN, which shows attenuated virulence and is unable to grow in healthy wild-type flies but grows normally in the immunocompromised PGRP-SA^seml mutants.

(Reed et al., 2015)

PGRP-SA^seml mutant flies show an increased susceptibility to challenge by Staphylococcus aureus or Enterococcus faecalis compared to wild type flies.

(Geuking et al., 2009)

Mutant flies are susceptible to S. aureus infection compared to wild-type adults.

(Wang et al., 2008)

Hemolymph clots from PGRP-SA^seml third instar larvae show normal melanization.

(Bidla et al., 2007)

PGRP-SA^seml mutant adults phagocytose E.coli normally, but phagocytosis of S.aureus is reduced.

(Garver et al., 2006)

Mutant flies show similar survival rates as control flies following natural infection (feeding with contaminated food) with either "Ecc-15" (P.carotovorum.carotovorum), S.cerevisiae or M.luteus.

(Ha et al., 2005)

PGRP-SA^seml flies show a normal survival rate after natural infection with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Mutant flies show reduced survival compared to wild-type controls after infection with either S. pyogenes or S. aureus.

(Bischoff et al., 2004)

PGRP-SA^seml flies are susceptible to infection by Gram-positive bacteria (E.faecalis, S.aureus or M.luteus). PGRP-SA^seml flies are resistant to fungal infection (A.fumigatus or B.bassiana) and to infection by Gram-negative bacteria (P.carotovorum.carotovorum).

(Pili-Floury et al., 2004)

Mutants are highly susceptible to Gram-positive infection, but are resistant as wild-type to fungal and Gram-negative bacterial infection.

(Michel et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

PGRP-SA^seml has abnormal immune response | adult stage phenotype, non-enhanceable by PGRP-SD^sk1

(Iatsenko et al., 2016)

PGRP-SA^seml has abnormal immune response phenotype, non-enhanceable by Scer\GAL4^da.G32/GNBP1^RNAi.UAS

(Pili-Floury et al., 2004)

PGRP-SA^seml has abnormal immune response phenotype, non-enhanceable by GNBP1^e03371

(Gobert et al., 2003)

PGRP-SA^seml has abnormal immune response phenotype, non-enhanceable by nec¹/nec²

(Michel et al., 2001)

NOT suppressed by

Statement

Reference

PGRP-SA^seml has abnormal immune response phenotype, non-suppressible by nec¹/nec²

(Michel et al., 2001)

NOT Enhancer of

Statement

Reference

PGRP-SA^seml is a non-enhancer of abnormal immune response phenotype of GNBP1^RNAi.UAS, Scer\GAL4^da.G32

(Pili-Floury et al., 2004)

PGRP-SA^seml is a non-enhancer of abnormal immune response phenotype of GNBP1^e03371

(Gobert et al., 2003)

PGRP-SA^seml is a non-enhancer of melanotic necrosis phenotype of nec¹/nec²

(Michel et al., 2001)

NOT Suppressor of

Statement

Reference

PGRP-SA^seml is a non-suppressor of melanotic necrosis phenotype of nec¹/nec²

(Michel et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

PGRP-SA^seml is a non-enhancer of adult epidermis phenotype of nec¹/nec²

(Michel et al., 2001)

NOT Suppressor of

Statement

Reference

PGRP-SA^seml is a non-suppressor of adult tracheal system phenotype of Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS

(Tang et al., 2008)

PGRP-SA^seml is a non-suppressor of adult epidermis phenotype of nec¹/nec²

(Michel et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced survival rate of PGRP-SA^seml mutant adults upon infection with either Staphylococcus aureus or Streptococcus pyogenes is not worsened further by combination with PGRP-SD^sk1.

(Iatsenko et al., 2016)

The melanisation of the adult tracheal system caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS is not suppressed by PGRP-SA^seml.

(Tang et al., 2008)

PGRP-SD^Δ3 PGRP-SA^seml and GNBP1^e03371 PGRP-SA^seml double mutant flies are extremely susceptible to S. aureus infection compared to wild-type adults.

(Wang et al., 2008)

Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 in a PGRP-SA^seml background show the same degree of susceptibility to M.luteus as either PGRP-SA^seml single mutant flies or flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background.

(Pili-Floury et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PGRP-SA^seml is rescued by PGRP-SA^UAS.cMa/Scer\GAL4^da.G32

(Michel et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55716

P{Dipt2.2-lacZ}1, P{Drs-GFP.JM804}1, y¹ w^* PGRP-SA^seml

Notes on Origin

Discoverer

Comments

Etymology: The mutation semmelweis is named after Ignaz Philipp Semmelweis, a Hungarian physician who was a pioneer in the field of antiseptic treatments and discovered the cause of puerperal fever.

(Michel et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PGRP-SA^Seml

(Aymeric et al., 2010)

PGRP-SA^seml

(Bahuguna et al., 2022, Iatsenko et al., 2020, Dudzic et al., 2019, Troha et al., 2019, Iatsenko et al., 2016, Reed et al., 2015, Arefin et al., 2014, Schmidt et al., 2011, Buchon et al., 2009, Geuking et al., 2009, Davis et al., 2008, Wang et al., 2008, Bidla et al., 2007, Bischoff et al., 2006, Jang et al., 2006, Pelte et al., 2006, Wang et al., 2006, Bischoff et al., 2004)

seml

(Tang et al., 2008, Gottar et al., 2002, Michel et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (30)

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