FlyBase Allele Report: Dmel\nec[2]

General Information

Symbol

Dmel\nec²

Species

D. melanogaster

Name

FlyBase ID

FBal0035260

Feature type

allele

Associated gene

Dmel\nec

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Heitzler et al., 1993)

Mutagen

ethyl methanesulfonate

(Heitzler et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q37term.

(Green et al., 2003, Green et al., 2000)

FlyBase curator comment: The authors mistakenly used the 3 letter code Glu instead of Gln when describing this mutation. This is evident from FBrf0131303 which states Q37term, and the fact that the nec protein has a long glutamine stretch from 31--39.

(Green et al., 2003)

Causes a truncation of the peptide within the polyglutamine repeat.

(Green et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:7,157,973..7,157,973 [-]

Nucleotide change:

C7157973T

Amino acid change:

Q37term | nec-PA

Reported amino acid change:

Q37term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Green et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with nec¹)

(Green et al., 2000)

lethal (with nec¹)

(Green et al., 2000)

lethal | recessive

(Green et al., 2000, Heitzler et al., 1993)

melanotic necrosis (with nec¹)

(Ramet et al., 2002, Michel et al., 2001, Green et al., 2000)

partially lethal - majority live | adult stage | heat sensitive (with nec¹)

(Green et al., 2003)

partially lethal - majority live | adult stage | heat sensitive (with nec⁹)

(Green et al., 2003)

short lived (with nec¹)

(Ligoxygakis et al., 2002)

short lived | heat sensitive (with nec¹)

(Green et al., 2003)

short lived | heat sensitive (with nec⁹)

(Green et al., 2003)

some die during immature adult stage | recessive (with nec¹)

(Green et al., 2000)

Phenotype Manifest In

adult abdomen (with nec¹)

(Green et al., 2000)

adult cuticle (with nec¹⁹)

(Green et al., 2003)

adult epidermis (with nec¹)

(Ramet et al., 2002, Michel et al., 2001, Green et al., 2000)

Detailed Description

Statement

Reference

The lifespan of nec¹/nec² adults is greatly reduced at 25[o]C or 29[o]C, compared to wild-type flies.

(Green et al., 2003)

80% of nec¹/nec² flies die within 48 hours of eclosion at 25^oC.

(Ligoxygakis et al., 2002)

nec¹/nec² mutant animals display melanotic spots on the cuticle.

(Michel et al., 2001)

nec¹/nec² flies survive for several days though the females are sterile. The nec mutant phenotype of Df(2R)sple-D2/Df(2R)nap2 is indistinguishable from that of nec¹/nec². Between 10 and 20% of nec¹/nec² larvae show brown spots around the posterior spiracles. Approximately 10% of the mutant pupae fail to eclose. Adult nec¹/nec² flies show a patchy distribution of melanotic spots of variable position and intensity on cuticular surfaces, but not internally. Their abdomens gradually swell with hemolymph and are extremely distended by 48 hrs post eclosion. The epidermal cells of adult nec¹/nec² flies undergo necrosis, in sites corresponding to the melanotic spots. An additional layer of healthy epidermal cells is seen beneath the necrotic cells. The necrotic mutant phenotype is pleiotropic and it is unclear what causes the lethality of adult nec mutant flies.

(Green et al., 2000)

Homozygotes are pharate adult lethal or die within 24 hours of eclosion.

(Heitzler et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

nec¹/nec² has melanotic necrosis phenotype, non-enhanceable by PGRP-SA^seml

(Michel et al., 2001)

Suppressed by

Statement

Reference

nec¹/nec² has short lived phenotype, suppressible by psh²

(Ligoxygakis et al., 2002)

nec¹/nec² has short lived phenotype, suppressible by psh³

(Ligoxygakis et al., 2002)

nec¹/nec² has short lived phenotype, suppressible by psh⁴

(Ligoxygakis et al., 2002)

nec¹/nec² has short lived phenotype, suppressible by psh⁵

(Ligoxygakis et al., 2002)

nec¹/nec² has short lived phenotype, suppressible by psh¹

(Ligoxygakis et al., 2002)

nec¹/nec² has lethal | adult stage phenotype, suppressible by spz⁴

(Green et al., 2000)

NOT suppressed by

Statement

Reference

nec¹/nec² has melanotic necrosis phenotype, non-suppressible by PGRP-SA^seml

(Michel et al., 2001)

NOT Enhancer of

Statement

Reference

nec¹/nec² is a non-enhancer of abnormal immune response phenotype of PGRP-SA^seml

(Michel et al., 2001)

NOT Suppressor of

Statement

Reference

nec¹/nec² is a non-suppressor of abnormal immune response phenotype of PGRP-SA^seml

(Michel et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

nec¹/nec² has adult epidermis phenotype, non-enhanceable by PGRP-SA^seml

(Michel et al., 2001)

NOT suppressed by

Statement

Reference

nec¹/nec² has adult epidermis phenotype, non-suppressible by PGRP-SA^seml

(Michel et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of PGRP-SA^seml to nec¹/nec¹ animals has no effect on either phenotypes.

(Michel et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nec¹/nec² is rescued by Scer\GAL4^da.G32/nec^UAS.cLa

(Green et al., 2000)

nec¹/nec² is rescued by Scer\GAL4^da.G32/nec^UAS.cLa

(Levashina et al., 1999)

Not rescued by

nec¹/nec² is not rescued by nec^PP.UAS/Scer\GAL4^da.G32

(Green et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9151

Dp(?;2)bw^D, nec² bw^D/CyO

67100

w^*; nec², P{GawB}c564; PBac{UAS-Spn27A.nec}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

nec²

(Katzenberger et al., 2013, Pelte et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (9)

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