FlyBase Allele Report: Dmel\Incenp[EP2340]

General Information

Symbol

Dmel\Incenp^EP2340

Species

D. melanogaster

Name

FlyBase ID

FBal0130978

Feature type

allele

Associated gene

Dmel\Incenp

Associated Insertion(s)

P{EP}Incenp^EP2340

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Incenp^EP2340

Cytology

Description

P{EP}Incenp^EP2340 is inserted into the third exon of Incenp.

(Chang et al., 2006)

P{EP} insertion at codon 91 in the Incenp coding region.

(Tseng and Hariharan, 2002)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,279,129..7,279,129 [+]

(Chang et al., 2006, Resnick et al., 2006, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | embryonic stage 5

(Tseng and Hariharan, 2002)

eye

(Chang et al., 2006)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

onion stage spermatid

(Chang et al., 2006)

spindle

(Chang et al., 2006)

wing

(Chang et al., 2006)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

Male flies carrying Incenp^EP2340 show a dominant meiotic phenotype that results in low fertility. The main defects incude misshapen meiotic spindles, tetrapolar or multipolar spindles and defects in onion stage spermatids consistent with problems both in chromosome disjunction and cytokinesis. Heterozygous individuals also show defects in larval mitosis and mild external defects consistent with abnormalities during imaginal mitosis, including slightly roughened eyes, nicked wings and defects in the abdominal terguites.

(Chang et al., 2006)

Heterozygotes show reduced fertility.

Mutant males show defects in meiosis I; cells are seen in which unaligned chromosomes are distributed along the spindle and cells in which the four bivalents are not distinguishable or the chromosome morphology is abnormal are seen.

Heterozygous males show increased nondisjunction of the sex, second and fourth chromosomes during meiosis compared to controls.

(Resnick et al., 2006)

No oocytes have been observed in females in which homozygous Incenp^EP2340 germline clones have been induced, suggesting that Incenp is required for early stages of oocyte development.

(Jang et al., 2005)

Expression of Incenp^EP2340 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing Incenp^EP2340 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing Incenp^EP2340 under the control of Scer\GAL4^en-e16E have wings with a smaller posterior compartment that contains a reduced number of cells. Cells from third larval instar wing discs expressing Incenp^EP2340 under the control of Scer\GAL4^Act5C.PP are normal in size. There is a small increase in the proportion of cells with a 4N DNA content (cells presumed to be in G2) compared to controls. Homozygous blastoderm embryos have irregular cell outlines after cellularisation and appear to contain increased amounts of DNA.

(Tseng and Hariharan, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Incenp^EP2340, Scer\GAL4^ey.PH has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Other

Statement

Reference

EC201^EC201, Incenp^EP2340 has lethal | embryonic stage phenotype

(Chang et al., 2006)

EC1650^EC1650, Incenp^EP2340 has partially lethal - majority die phenotype

(Chang et al., 2006)

EC2364^EC2364, Incenp^EP2340 has viable phenotype

(Chang et al., 2006)

EC2374^EC2374, Incenp^EP2340 has viable phenotype

(Chang et al., 2006)

EC4056^EC4056, Incenp^EP2340 has partially lethal - majority die phenotype

(Chang et al., 2006)

EC2388^EC2388, Incenp^EP2340 has viable phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has viable phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has abnormal meiotic cell cycle phenotype

(Chang et al., 2006)

EC2519^EC2519, Incenp^EP2340 has partially lethal - majority die phenotype

(Chang et al., 2006)

EC3959^EC3959, Incenp^EP2340 has partially lethal - majority die phenotype

(Chang et al., 2006)

EC282^EC282, Incenp^EP2340 has partially lethal - majority die | embryonic stage phenotype

(Chang et al., 2006)

EC497^EC497, Incenp^EP2340 has partially lethal - majority die | first instar larval stage phenotype

(Chang et al., 2006)

EC549^EC549, Incenp^EP2340 has partially lethal - majority die | embryonic stage phenotype

(Chang et al., 2006)

EC666^EC666, Incenp^EP2340 has abnormal meiotic cell cycle phenotype

(Chang et al., 2006)

EC666^EC666, Incenp^EP2340 has lethal | third instar larval stage phenotype

(Chang et al., 2006)

EC1608^EC1608, Incenp^EP2340 has viable phenotype

(Chang et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Incenp^EP2340, Scer\GAL4^ey.PH has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Other

Statement

Reference

EC282^EC282, Incenp^EP2340 has macrochaeta phenotype

(Chang et al., 2006)

EC2388^EC2388, Incenp^EP2340 has macrochaeta phenotype

(Chang et al., 2006)

EC2388^EC2388, Incenp^EP2340 has wing phenotype

(Chang et al., 2006)

EC2388^EC2388, Incenp^EP2340 has eye phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has macrochaeta phenotype

(Chang et al., 2006)

EC282^EC282, Incenp^EP2340 has wing phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has wing phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has eye phenotype

(Chang et al., 2006)

EC2394^EC2394, Incenp^EP2340 has primary spermatocyte phenotype

(Chang et al., 2006)

EC2519^EC2519, Incenp^EP2340 has macrochaeta phenotype

(Chang et al., 2006)

EC2519^EC2519, Incenp^EP2340 has wing phenotype

(Chang et al., 2006)

EC2519^EC2519, Incenp^EP2340 has eye phenotype

(Chang et al., 2006)

EC3959^EC3959, Incenp^EP2340 has macrochaeta phenotype

(Chang et al., 2006)

EC3959^EC3959, Incenp^EP2340 has wing phenotype

(Chang et al., 2006)

EC282^EC282, Incenp^EP2340 has eye phenotype

(Chang et al., 2006)

EC3959^EC3959, Incenp^EP2340 has eye phenotype

(Chang et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Variation in the nuclear size in EC2519^EC2519/Incenp^EP2340 is an indication of defective chromokinesis.

The presence of giant primary spermatocytes in EC2394^EC2394/Incenp^EP2340 could be indicative of a defect in cytokinesis in gonial mitosis.

Abnormal meiotic spindles and tetrapolar spindles are found in EC2394^EC2394/Incenp^EP2340 mutants.

(Chang et al., 2006)

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of Incenp^EP2340 under the control of Scer\GAL4^ey.PH. Cells from third larval instar wing discs expressing Incenp^EP2340 under the control of Scer\GAL4^Act5C.PP (and also co-expressing BacA\p35^Scer\UAS.cHa to eliminate cell death) have a 15% longer doubling time than control cells expressing BacA\p35^Scer\UAS.cHa alone under the control of Scer\GAL4^Act5C.PP.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Incenp^EP2340

(Jang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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