CanB2^EP774/+ does not cause significant cardiac enlargement or reduced fractional shortening in adult hearts.

Mutant third instar larvae are capable of synaptic homeostasis at the neuromuscular junction, as indicated by an increase in quantal content in the presence of the glutamate receptor antagonist philanthotoxin-433 (10μM).

Mature oocytes ofCanB2^EP774 germ-line clones appear to have normal metaphase I chromosome configuration. However, the laid eggs fail to hatch. The chromosomes in these eggs exhibit an arrested meiotic anaphase I-like configuration.

Over 70% of mid-stage CanB2^EP774 pupae show collapse of all indirect flight muscles. In the remaining 30%, no pupa has more than 7 uncollapsed DLMs (dorsal longitudinal muscles, also known as dorsal medial muscles).

CanB2^EP774 indirect flight muscle tissues show a range of phenotypes. In CanB2^EP774 adults, 20% of myofibrils have a wild-type appearance, ~40% exhibit no discernable filament structure in central regions but have some scattered organized filament patches at the periphery. The remaining mutant adults lack myofibrils with any recognizable structures. CanB2^EP774 adult IFMs sometimes have short Z-bands and missing or partial M-lines, indicative of a reduction or absence of thick filaments. CanB2^EP774 pupae show more severe phenotypes than adults, as their IFMs show no organized filament structure and their most severely-affected muscles have no Z-bands or any other sarcomeric structure markers.

Small numbers of CanB2^EP774 animals survive to adulthood. These animals are flightless, with wings positioned at abnormal angles. In CanB2^EP774 homozygous, CanB2^EP774/CanB2¹⁸⁰ and CanB2^EP774/Df(2R)ST1 animals, large gaps or deletions occur in the centrally located dorsal medial muscle pairs. In most cases, one or more dorsal muscles are present, and on rare occasions, one or more ventral muscles. However, the dorsal ventral and trochanter muscle pairs are intact and normally arranged in these flies. In contrast to the precisely arrayed indirect flight muscles present in posterior thoraces of wild-type animals, the posterior thoraces of CanB2^EP774 homozygous, CanB2^EP774/CanB2¹⁸⁰ and CanB2^EP774/Df(2R)ST1 animals contain large, poorly defined muscle masses. Indirect flight muscle formation in CanB2^EP774 homozygotes is normal in day 2 pupae. During pupal day 3 there is a slow, progressive retraction of the indirect flight muscles towards the posterior of the thorax after they initiate observable muscle contractions. By day 4 all dorsal medial muscle and dorsal ventral muscles are mis-located in a posterior thoracic position.

CanB2^EP774/CanB2[+] is a suppressor of abnormal size | adult stage phenotype of Pp2B-14D^act.UAS.EYFP, Scer\GAL4^tin.CΔ4

CanB2^EP774/CanB2[+] is a suppressor | partially of female sterile phenotype of Pp2B-14D^act.UAS, Scer\GAL4^{VP16.nanos.UTR}

CanB2^EP774/CanB2[+], Scer\GAL4^how-24B is a suppressor of lethal | pupal stage phenotype of Mmus\Ppp3ca^Act.UAS, Scer\GAL4^how-24B