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General Information
Symbol
Dmel\Pp2B-14Dact.UAS.EYFP
Species
D. melanogaster
Name
FlyBase ID
FBal0320395
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of constitutively active Pp2B-14D (lacks the C-terminal portion of the protein, including the autoinhibitory domain) that is tagged at the N-terminal end with EYFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
model of  cardiomyopathy
is ameliorated by styΔ5
is ameliorated by Mef2X1
is ameliorated by CanB2EP774
is ameliorated by GalkMB10638
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4tin.CΔ4 in adults results in cardiac enlargement, reduced fractional shortening, and significantly thicker heart wall, as compared to controls.

Expression of Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4en-e16E results in abnormalities in the posterior crossvein and longitudinal vein 5.

Expression of Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4Act5C.PI results in larval lethality.

Expression of Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4dpp.blk1 results in failure of the wing to expand.

Expression of Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4Mef2.PR results in lethality in P-stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Mef2X1/+, CanB2EP774/+, Df(3L)ED4416/+ or Df(3L)BSC130/+ (but not styΔ5/+) rescues the cardiac enlargement of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4tin.CΔ4. Mef2X1/+, styΔ5/+, CanB2EP774/+ or Df(3L)ED4416/+ (but not Df(3L)BSC130/+) rescues the reduced fractional shortening phenotype of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4tin.CΔ4.

GalkMB10638/+ rescues the increased heart wall thickness phenotype of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4tin.CΔ4.

Df(3L)ED4416/+ or GalkMB10638/+ partially rescues the abnormal wing vein phenotypes of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4en-e16E.

Df(3L)ED4416/+ fails to rescue the lethality of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4Act5C.PI or Scer\GAL4Mef2.PR, and fails to rescue the wing expansion phenotype of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4dpp.blk1.

GalkMB10638/+ fails to rescue the lethality of flies expressing Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP under the control of Scer\GAL4Mef2.PR.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pp2B-14Dact.Scer\UAS.T:Avic\GFP-EYFP
Pp2B-14Dact.UAS.EYFP
Name Synonyms
Secondary FlyBase IDs
    References (1)