The overall organisation and branching pattern of the embryonic tracheal system is unaffected in bou6Ea-2/bouPG27 trans-heterozygous mutants. However, both the shape and the length of the tracheal dorsal trunk segments are abnormal in mutant flies. Differing from the wild-type, this structure appears in stage 15 embryos as a succession of bulging cysts and, by stage 16, as an abnormally convoluted tube. The fibrous structure of the tracheal chitin cable is disorganised in mutant embryos and chitin is deposited as an amorphous material in the tracheal lumen.
The tracheal tubes are abnormal in shape and dimensions in mutant embryos.
Large homozygous clones in the wing (induced early in larval development) do not show any obvious growth defects.
bouPG27 is rescued by Scer\GAL4bou-PG27/bouUAS.Tag:HA
bouPG27 is not rescued by Scer\GAL4bou-PG27/bouΔC.UAS.Tag:HA
Excision of the insertion reverts the lethal phenotype.