FlyBase Allele Report: Dmel\usp[GAL4::LBD.hs]

General Information

Symbol

Dmel\usp^GAL4::LBD.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0137065

Feature type

allele

Associated gene

Dmel\usp

Associated Insertion(s)

Carried in Construct

P{hs-GAL4-usp.LBD}

Also Known As

hs-GAL4-USP, hs-GAL4-usp.LBD

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kozlova and Thummel, 2002)

Progenitor genotype

Carried in construct

P{hs-GAL4-usp.LBD}

Cytology

Description

An Hsp70 promoter drives expression of a ligand-inducible transcriptional activator that is composed of the Scer\GAL4 DNA binding domain fused to amino acids 170-508 of usp (this encodes the ligand binding domain and hinge region).

(Kozlova and Thummel, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

GAL4::LBD

binary expression system - small molecule-regulated driver

small molecule sensor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S9

centripetal follicle cell

(Jang et al., 2009)

stretch follicle cell

(Jang et al., 2009)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Kucherenko et al., 2012)

lethal - all die before end of larval stage | partially | heat sensitive

(Kozlova and Thummel, 2002)

some die during larval stage | partially | heat sensitive

(Kozlova and Thummel, 2002)

Phenotype Manifest In

adult mushroom body beta-lobe

(Kucherenko et al., 2012)

female germline cell | increased number | heat sensitive

(König et al., 2011)

lobe system of adult mushroom body

(Kucherenko et al., 2012)

Detailed Description

Statement

Reference

Animals expressing usp^{hs.T:Scer\GAL4} have defects in the mushroom body α/β lobes: the lobes may appear slim or occasionally underdeveloped, axonal projections may be misguided or the β lobes may be fused.

(Kucherenko et al., 2012)

Expression of usp^{hs.T:Scer\GAL4} using heat shock results in germaria that are filled with supernumerary germline cells containing a single spectrosome (SSCs) in females. The ratio between developing fusome-containing cells and SSCs in regions 1-2A of the germaria is decreased in the mutants compared to wild type.

(König et al., 2011)

Expression of usp^{hs.T:Scer\GAL4} using heat shock in late third instar larvae results in 30-40% lethality. Animals carrying usp^{hs.T:Scer\GAL4} and subjected to heat shock during the early pupal stage (12-20 hours after puparium formation) almost all survive.

(Kozlova and Thummel, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

usp^GAL4::LBD.hs has abnormal neuroanatomy phenotype, suppressible by mir-let7^UAS.cCa

(Kucherenko et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

usp^GAL4::LBD.hs has lobe system of adult mushroom body phenotype, suppressible by mir-let7^UAS.cCa

(Kucherenko et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of let-7^Scer\UAS.cCa rescues the mushroom body α/β lobe defects seen in animals expressing usp^{hs.T:Scer\GAL4}.

(Kucherenko et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28864

w¹¹¹⁸; P{UAS-Stinger}2; P{hs-GAL4-usp.LBD}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

usp^GAL4::LBD.hs

usp^{hs.T:Scer\GAL4}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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