FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\uspGAL4::LBD.hs
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General Information
Symbol
Dmel\uspGAL4::LBD.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0137065
Feature type
allele
Associated gene
Dmel\usp
Associated Insertion(s)
Carried in Construct
P{hs-GAL4-usp.LBD}
Also Known As
hs-GAL4-USP, hs-GAL4-usp.LBD
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kozlova and Thummel, 2002)
Progenitor genotype
Carried in construct
P{hs-GAL4-usp.LBD}
Cytology
Description

An Hsp70 promoter drives expression of a ligand-inducible transcriptional activator that is composed of the Scer\GAL4 DNA binding domain fused to amino acids 170-508 of usp (this encodes the ligand binding domain and hinge region).

(Kozlova and Thummel, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
GAL4::LBD
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      oogenesis stage S9
      centripetal follicle cell
      (Jang et al., 2009)
      stretch follicle cell
      (Jang et al., 2009)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing usphs.T:Scer\GAL4 have defects in the mushroom body α/β lobes: the lobes may appear slim or occasionally underdeveloped, axonal projections may be misguided or the β lobes may be fused.

      (Kucherenko et al., 2012)

      Expression of usphs.T:Scer\GAL4 using heat shock results in germaria that are filled with supernumerary germline cells containing a single spectrosome (SSCs) in females. The ratio between developing fusome-containing cells and SSCs in regions 1-2A of the germaria is decreased in the mutants compared to wild type.

      (König et al., 2011)

      Expression of usphs.T:Scer\GAL4 using heat shock in late third instar larvae results in 30-40% lethality. Animals carrying usphs.T:Scer\GAL4 and subjected to heat shock during the early pupal stage (12-20 hours after puparium formation) almost all survive.

      (Kozlova and Thummel, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      uspGAL4::LBD.hs has abnormal neuroanatomy phenotype, suppressible by mir-let7UAS.cCa

      (Kucherenko et al., 2012)
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of let-7Scer\UAS.cCa rescues the mushroom body α/β lobe defects seen in animals expressing usphs.T:Scer\GAL4.

      (Kucherenko et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The usphs.T:Scer\GAL4 protein produced can bind to Scer\UAS regulatory sequences in the absence of ligand, but should not activate transcription. In the presence of ligand the protein can switch to an active conformation and induce expression from the Scer\UAS regulatory sequences.

      (Kozlova and Thummel, 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      uspGAL4::LBD.hs
      usphs.T:Scer\GAL4
      Name Synonyms
      Secondary FlyBase IDs
        References (7)