FlyBase Allele Report: Dmel\nanos[αTub84B.3'UTR.nanos+2.UAS]

General Information

Symbol

Dmel\nanos^{αTub84B.3'UTR.nanos+2.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0141014

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

P{UAS-nanos-tub:nanos+2}

Also Known As

UAS-nos-tub:nos+2

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Clark et al., 2002)

Progenitor genotype

nanos^{αTub84B.3'UTR.UAS}

(Clark et al., 2002)

Carried in construct

P{UAS-nanos-tub:nanos+2}

Cytology

Description

A 180bp nucleotide region of the nos 3'UTR (the "+2 element") has been inserted into a BstEII site within the αTub84B 3'UTR sequences.

(Clark et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nanos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^8-123

(Ye et al., 2004)

lethal, with Scer\GAL4^h-1J3

(Clark et al., 2002)

lethal, with Scer\GAL4^how-24B

(Clark et al., 2002)

partially lethal, with Scer\GAL4^elav.PLu

(Clark et al., 2002)

partially lethal, with Scer\GAL4^insc-Mz1407

(Clark et al., 2002)

partially lethal - majority die, with Scer\GAL4^HD34A

(Duchow et al., 2005)

visible, with Scer\GAL4^GMR.PF

(Clark et al., 2002)

visible, with Scer\GAL4^hs.2sev

(Clark et al., 2002)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^Eh.2.4

(Clark et al., 2002)

adult cuticle, with Scer\GAL4^HD23A

(Clark et al., 2002)

adult cuticle, with Scer\GAL4^HD44A

(Clark et al., 2002)

adult cuticle, with Scer\GAL4^hs.2sev

(Clark et al., 2002)

adult cuticle, with Scer\GAL4^sca-109-68

(Clark et al., 2002)

dendritic arborising neuron & dendritic tree, with Scer\GAL4^8-123

(Ye et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Clark et al., 2002)

ommatidium, with Scer\GAL4^hs.2sev

(Clark et al., 2002)

wing, with Scer\GAL4^Eh.2.4

(Clark et al., 2002)

wing, with Scer\GAL4^HD23A

(Clark et al., 2002)

wing, with Scer\GAL4^HD44A

(Clark et al., 2002)

wing, with Scer\GAL4^hs.2sev

(Clark et al., 2002)

wing, with Scer\GAL4^sca-109-68

(Clark et al., 2002)

Detailed Description

Statement

Reference

Expression of nos^{αTub84B.3'UTR.nos+2.Scer\UAS} in the dorsal pouch epithelium, under the control of Scer\GAL4^HD34A, causes lethality in 25% of flies.

(Duchow et al., 2005)

Expression of nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^8-123 results in a reduction in the amount of high-order dendritic branches in class IV dendritic arborization neurons of third instar larvae, but the phenotype is significantly less severe than that seen when nos^{αTub84B.3'UTR.Scer\UAS} is expressed under the control of Scer\GAL4^8-123.

(Ye et al., 2004)

100% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^GMR.PF have rough eyes. These flies have black spots. 100% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^hs.2sev have rough eyes. Flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^elav.PLu show 84% lethality. Flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4¹⁴⁰⁷ show 61% lethality. 24% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^hs.2sev show an "adolescent" phenotype; the wing expansion and body cuticle hardening that normally occurs shortly after eclosion fails to occur. The flies are fertile, but show reduced viability. A number of other Scer\GAL4 lines produce this "adolescent" phenotype in combination with nos^{αTub84B.3'UTR.nos+2.Scer\UAS}, they are: Scer\GAL4^sca-109-68 (23% penetrance), Scer\GAL4^HD23A (33% penetrance), Scer\GAL4^HD44A (24% penetrance) and Scer\GAL4^Eh.2.4 (75% penetrance).

(Clark et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.nanos+2.UAS} has wing phenotype, non-suppressible by smg¹

(Clark et al., 2002)

Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.nanos+2.UAS} has adult cuticle phenotype, non-suppressible by smg¹

(Clark et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of the adolescent phenotype in animals expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^sca-109-68 is not altered if they are also carrying smg¹.

(Clark et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

nanos^{αTub84B.3'UTR.nanos+2.UAS}

nanos^{αTub84B.3'UTR.nos+2.UAS}

nos^{αTub84B.3'UTR.nos+2.Scer\UAS}

nos^{αTub84B.3'UTR.nos+2.UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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