FlyBase Allele Report: Dmel\Vap33[Δ448]

General Information

Symbol

Dmel\Vap33^Δ448

Species

D. melanogaster

Name

FlyBase ID

FBal0141327

Feature type

allele

Associated gene

Dmel\Vap33

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Pennetta et al., 2002)

Mutagen

P-element activity

(Pennetta et al., 2002)

Progenitor genotype

Vap33⁴⁷

(Pennetta et al., 2002)

Cytology

Description

Imprecise excision of P{lArB} of Vap-33-1⁴⁷ removing 1.5kb of flanking DNA, including Vap-33-1 coding region.

(Pennetta et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Karsten et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Pennetta et al., 2002)

abnormal neurophysiology

(Chai et al., 2008)

lethal - all die before end of larval stage

(Chai et al., 2008)

lethal - all die before end of larval stage | recessive

(Pennetta et al., 2002)

some die during larval stage

(Chai et al., 2008)

some die during larval stage | recessive

(Pennetta et al., 2002)

Phenotype Manifest In

adult mushroom body alpha-lobe | pupal stage

(Tsuda et al., 2008)

bouton

(Pennetta et al., 2002)

bouton & microtubule

(Pennetta et al., 2002)

NMJ bouton

(Chai et al., 2008)

synapse

(Chai et al., 2008)

Detailed Description

Statement

Reference

Vap-33-1^Δ448 mutants are larval lethal with rare adult escapers (~1%).

(Chai et al., 2008)

Vap-33-1^Δ448 mutants lack mushroom body α lobes in late pupae and adult brains.

(Tsuda et al., 2008)

Lethality acts in the 2nd/early 3rd instar. Number of boutons on embryonic somatic muscles is 50% wild type, though muscle surface area is normal. The size of the boutons is abnormally large, such that synaptic surface area for all boutons on a muscle is similar to that of wild type. Microtubules in boutons are disorganised, and punctae are evident. Localization of syt, Csp, Fas2 and dlg1 proteins is normal, indicating normal axonal transport.

(Pennetta et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vap33^Δ448 has lethal | larval stage phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ448 has abnormal neurophysiology phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ448 has lethal | larval stage phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Vap33^Δ448 has abnormal neurophysiology phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Vap33^Δ448 has lethal phenotype, suppressible by Hsap\VAPB^P56S.UAS/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ448 has lethal phenotype, suppressible by Hsap\VAPB^P56S.UAS/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Suppressor of

Statement

Reference

Vap33^Δ448/Vap-33-1[+] is a suppressor of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Karsten et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vap33^Δ448 has NMJ bouton phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ448 has synapse phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ448 has NMJ bouton phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Vap33^Δ448 has synapse phenotype, suppressible by Hsap\VAPB^UAS.cCa/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Suppressor of

Statement

Reference

Vap33^Δ448/Vap-33-1[+] is a suppressor of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Karsten et al., 2006)

Other

Statement

Reference

Eph^unspecified, Vap33^Δ448 has adult mushroom body alpha-lobe | pupal stage phenotype

(Tsuda et al., 2008)

Eph^unspecified, Vap33^Δ448 has adult mushroom body alpha-lobe | adult stage phenotype

(Tsuda et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Vap-33-1^Δ448 Eph^unspecified double mutants lack mushroom body α lobes in late pupae and adult brains.

(Tsuda et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\VAPB^Scer\UAS.cCa in a Vap-33-1^Δ448 mutant background, under the control of Scer\GAL4^C164 or Scer\GAL4^D42 rescues the larval lethality. Rescued flies are fertile and do not show any obvious morphological or behavioral defects.

In synapses expressing Hsap\VAPB^Scer\UAS.cCa under the control of Scer\GAL4^C164 or Scer\GAL4^D42 in a Vap-33-1^Δ448 background, the number of synaptic boutons is similar to controls. Electrophysiological analysis of the same synapses shows that the EJPs and mEJPs are both similar to controls. Hence, the lethality, aberrant NMJ morphology and increased mEJP amplitude associated with loss of Vap-33-1 are rescued by Hsap\VAPB^Scer\UAS.cCa neuronal expression.

Vap-33-1^Δ448 mutant lethality is rescued through expression of Hsap\VAPB^{P56S.Scer\UAS} under the control of either Scer\GAL4^C164 or Scer\GAL4^D42. However, the number of boutons is not significantly different in flies expressing Hsap\VAPB^{P56S.Scer\UAS} compared to controls. Moreover, no significant difference in EJPs or quantal sizes is observed in flies expressing Hsap\VAPB^{P56S.Scer\UAS}.

(Chai et al., 2008)

The rough eye phenotype caused by Hsap\MAPT^GMR.Ex.PJ is suppressed by Vap-33-1^Δ448/+.

(Karsten et al., 2006)

Complementation and Rescue Data

Rescued by

Vap33^Δ448 is rescued by Vap33^UAS.cPa/Scer\GAL4^C164

(Pennetta et al., 2002)

Vap33^Δ448 is rescued by Vap33^hs.PP

(Pennetta et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39683

Vap33^Δ448/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DVAP-33^Δ448

(Chai et al., 2008)

DVAP33A⁴⁴⁸

(Karsten et al., 2006)

Vap-33-1^Δ448

Vap-33A^Δ448

Vap33^Δ448

(Li et al., 2025)

dVAP^Δ488

(Tsuda et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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