FlyBase Allele Report: Dmel\Sirt1[05327a]

General Information

Symbol

Dmel\Sirt1^05327a

Species

D. melanogaster

Name

FlyBase ID

FBal0141418

Feature type

allele

Associated gene

Dmel\Sirt1

Associated Insertion(s)

P{PZ}Sirt1^05327a

Carried in Construct

Also Known As

DSir2⁰⁵³²⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Sirt1^05327a

Cytology

Description

P{PZ} insertion 460bp 5' of the Sir2 initiation codon, within the 5' UTR.

(Furuyama et al., 2004)

Insertion approximately 400bp upstream of the Sir2 start codon.

(Astrom et al., 2003)

P{PZ} insertion at position +14 of the Sir2 mRNA.

(Rosenberg and Parkhurst, 2002)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:13,165,538..13,165,538

(Tulin et al., 2006, Furuyama et al., 2004, Astrom et al., 2003, Newman et al., 2002, Rosenberg and Parkhurst, 2002, Fernandez-Funez et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sir2[+]/Sirt1^05327a is an enhancer of visible | homeotic phenotype of E(z)³²/E(z)⁶¹

(Furuyama et al., 2004)

Other

Statement

Reference

Sirt1^05327a, hry²⁶ has lethal | dominant | maternal effect phenotype

(Rosenberg and Parkhurst, 2002)

Polr2B^wimp, Sirt1^05327a has lethal | dominant | maternal effect | embryonic stage phenotype

(Rosenberg and Parkhurst, 2002)

Sirt1^05327a, h[+]/hry²⁶ has lethal | dominant | maternal effect phenotype

(Rosenberg and Parkhurst, 2002)

RpII140[+]/Polr2B^wimp, Sirt1^05327a has lethal | dominant | maternal effect | embryonic stage phenotype

(Rosenberg and Parkhurst, 2002)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Sirt1^05327a has retina phenotype, non-enhanceable by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Sirt1^05327a has eye phenotype, non-enhanceable by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT suppressed by

Statement

Reference

Sirt1^05327a has eye phenotype, non-suppressible by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Sirt1^05327a has retina phenotype, non-suppressible by Hsap\ATXN1^82Q.UAS/Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT Enhancer of

Statement

Reference

Sir2[+]/Sirt1^05327a is a non-enhancer of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Sirt1^05327a is a non-enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Sirt1^05327a is a non-enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT Suppressor of

Statement

Reference

Sir2[+]/Sirt1^05327a is a non-suppressor of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Sirt1^05327a is a non-suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Sirt1^05327a is a non-suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Other

Statement

Reference

Polr2B^wimp, Sirt1^05327a has ventral denticle belt phenotype

(Rosenberg and Parkhurst, 2002)

Polr2B^wimp, Sirt1^05327a has embryonic segment phenotype

(Rosenberg and Parkhurst, 2002)

RpII140[+]/Polr2B^wimp, Sirt1^05327a has ventral denticle belt phenotype

(Rosenberg and Parkhurst, 2002)

RpII140[+]/Polr2B^wimp, Sirt1^05327a has embryonic segment phenotype

(Rosenberg and Parkhurst, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from Sir2^05327a/+ ; RpII140^wimp/+ females (mated to wild-type males) die and show anterior-posterior patterning defects, including loss of segments and pairwise denticle band fusions. Sir2^05327a does not show a dominant synthetic lethal interaction with Rpd3⁰⁴⁵⁵⁶, gro^E47, eve^1.27, run³ or CtBP⁰³⁴⁶³.

(Rosenberg and Parkhurst, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Separable from: l(2)05327b^05327b. The lethality of the "l(2)05327" chromosome is not due to the P{PZ}Sir2^05327a insertion, but is due to a second site lethal (l(2)05327b^05327b).

(Newman et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DSir2⁰⁵³²⁷

(Miotto et al., 2006)

Sir2⁰⁵³²⁷

(Furuyama et al., 2004)

Sir2^05327a

Sirt1^05327a

dSir2⁰⁵³²⁷

(Fernandez-Funez et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBal0008083

References (11)

Report Sections

Open Close