FlyBase Allele Report: Dmel\Tfb5[SH1279]

General Information

Symbol

Dmel\Tfb5^SH1279

Species

D. melanogaster

Name

FlyBase ID

FBal0143719

Feature type

allele

Associated gene

Dmel\Tfb5

Associated Insertion(s)

P{lacW}Tfb5^SH1279

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Herrera-Cruz et al., 2012)

Mutagen

P-element activity

(Oh et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{lacW}Tfb5^SH1279

Cytology

Description

Insertion in the middle of the Tfb5 open reading frame, in the second exon.

(Herrera-Cruz et al., 2012)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:5,010,592..5,010,800

(Herrera-Cruz et al., 2012, Oh et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing

(Herrera-Cruz et al., 2012)

wing (with Df(2L)BSC172)

(Herrera-Cruz et al., 2012)

wing (with Df(2L)Exel7022)

(Herrera-Cruz et al., 2012)

Detailed Description

Statement

Reference

Homozygous, Tfb5^SH1279/Df(2L)Exel7022 and Tfb5^SH1279/Df(2L)BSC172 flies show semi-lethality. The surviving males are sterile, while the surviving females have very reduced fertility, laying very few fertilised eggs of which only very few develop. All of the adults have a held-out wing phenotype as well as a slight reduction in wing size.

(Herrera-Cruz et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Tfb5^SH1279 has partially lethal phenotype, non-suppressible by CG31917^{Hsp83.N.Tag:MYC}

(Herrera-Cruz et al., 2012)

Tfb5^SH1279 has male sterile phenotype, non-suppressible by CG31917^{Hsp83.N.Tag:MYC}

(Herrera-Cruz et al., 2012)

Tfb5^SH1279 has female semi-sterile phenotype, non-suppressible by CG31917^{Hsp83.N.Tag:MYC}

(Herrera-Cruz et al., 2012)

Tfb5^SH1279 has visible phenotype, non-suppressible by CG31917^{Hsp83.N.Tag:MYC}

(Herrera-Cruz et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Tfb5^SH1279 has wing phenotype, non-suppressible by CG31917^{Hsp83.N.Tag:MYC}

(Herrera-Cruz et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

CG31917^{Hsp83.N.T:Hsap\MYC} does not rescue viability or any other defects (sterility and held-out wing phenotype) seen in Tfb5^SH1279 homozygotes.

Flies expressing one copy of P{GD10407} (which results in down-regulation of both CG31917 and Tfb5 expression) under the control of one copy of Scer\GAL4^Bx-MS1096 in a homozygous Tfb5^SH1279 background show a reduction in wing size.

(Herrera-Cruz et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tfb5^SH1279 is rescued by Tfb5^{C.Hsp83.Tag:polyHis}

(Herrera-Cruz et al., 2012)

Comments

One copy of Tfb5^{C.Hsp83.T:Zzzz\His6} rescues the viability and all other defects (sterility and held-out wing phenotype) seen in Tfb5^SH1279 homozygotes.

(Herrera-Cruz et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The P{lacW}Tfb5^SH1279 insertion is within a bicistronic transcript that encodes both Tfb5 and CG31917. However, evidence indicates that the mutant phenotypes caused by the insertion are due to an effect on Tfb5 and not on CG31917 : all defects seen in P{lacW}Tfb5^SH1279 homozygotes are rescued by Tfb5^{C.Hsp83.T:Zzzz\His6} and CG31917^{Hsp83.N.T:Hsap\MYC} is unable to rescue any of the P{lacW}Tfb5^SH1279 mutant phenotypes.

(Herrera-Cruz et al., 2012)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tfb5^SH1279

l(2)SH1279

(Herrera-Cruz et al., 2012)

l(2)SH1279^SH1279

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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