FlyBase Allele Report: Dmel\ana3[SH0558]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\ana3^SH0558

General Information

Symbol

Dmel\ana3^SH0558

Species

D. melanogaster

Name

FlyBase ID

FBal0143965

Feature type

allele

Associated gene

Associated Insertion(s)

P{lacW}ana3^SH0558

Carried in Construct

Key Links

Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Stevens et al., 2009)

Mutagen

P-element activity

(Oh et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{lacW}ana3^SH0558

Cytology

Description

P{lacW} insertion 3 bp upstream of initiating ATG of ana3.

(Stevens et al., 2009)

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

reporter enzyme

Tagged with

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:12,344,981..12,345,083

(Stevens et al., 2009, Oh et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Oh et al., 2003)

(Stevens et al., 2009)

uncoordinated (with Df(2R)Exel6061)

(Stevens et al., 2009)

(Stevens et al., 2009)

viable (with Df(2R)Exel6061)

(Stevens et al., 2009)

Phenotype Manifest In

antennal segment 3 sense organ

(Stevens et al., 2009)

antennal segment 3 sense organ (with Df(2R)Exel6061)

(Stevens et al., 2009)

(Stevens et al., 2009)

axoneme (with Df(2R)Exel6061)

(Stevens et al., 2009)

(Stevens et al., 2009)

centriole (with Df(2R)Exel6061)

(Stevens et al., 2009)

(Stevens et al., 2009)

centrosome (with Df(2R)Exel6061)

(Stevens et al., 2009)

ciliary basal body

(Stevens et al., 2009)

ciliary basal body (with Df(2R)Exel6061)

(Stevens et al., 2009)

meiotic spindle

(Stevens et al., 2009)

meiotic spindle (with Df(2R)Exel6061)

(Stevens et al., 2009)

(Stevens et al., 2009)

spermatocyte (with Df(2R)Exel6061)

(Stevens et al., 2009)

Detailed Description

Statement

Reference

ana3^SH0558 and ana3^SH0558/Df(2R)Exel6061 flies are viable but severely uncoordinated and die shortly after eclosion as they become stuck in the food.

Basal bodies of sensory cilia in the pupal third antennal segment are undetectable in ana3^SH0558 and ana3^SH0558/Df(2R)Exel6061 animals. This leads to a lack of cilia in sensory neurons of these animals.

ana3^SH0558 and ana3^SH0558/Df(2R)Exel6061 larval brain cells show a large variation in centriole and centrosome number, with a third of cells having too few centrioles and centrosomes, and 44% of cells having too many. Some mutant centrioles recruit very small amounts of pericentriolar material, and these centrioles are smaller than normal.

Centriole numbers are reduced in ana3^SH0558 and ana3^SH0558/Df(2R)Exel6061 spermatocytes. In contrast to wild type, virtually none of the remaining centrioles are paired in the mutants. The prematurely separated centrioles remain competent to nucleate astral microtubules during meiosis, and this leads to multipolar spindles.

Centrioles and axonemes are structurally abnormal in ana3^SH0558 and ana3^SH0558/Df(2R)Exel6061 testes.

(Stevens et al., 2009)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ana3^SH0558 is rescued by ana3^Ubi.GFP

(Stevens et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Comments

Precision excision of the P{lacW} reverts the ana3^SH0558 phenotypes.

(Stevens et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ana3^SH0558

(Stevens et al., 2009)

ana3^SH0558

(Cho et al., 2021)

l(2)SH0558^SH0558

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections