FlyBase Allele Report: Dmel\step[SH0323]

General Information

Symbol

Dmel\step^SH0323

Species

D. melanogaster

Name

FlyBase ID

FBal0144079

Feature type

allele

Associated gene

Dmel\step

Associated Insertion(s)

P{lacW}step^SH0323

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Oh et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{lacW}step^SH0323

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,757,187..21,757,232

(Oh et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage (with step^k08110)

(Fuss et al., 2006)

decreased body size | larval stage

(Fuss et al., 2006)

decreased body size | larval stage (with step^k08110)

(Fuss et al., 2006)

lethal | recessive

(Oh et al., 2003)

Phenotype Manifest In

embryo | embryonic stage 5 (with step^k08110)

(Lee and Harris, 2013)

furrow canal (with step^k08110)

(Lee and Harris, 2013)

Detailed Description

Statement

Reference

Embryos derived from mothers maternally mutant for step^SH0323/step^k08110 have largely lost the endocytic tubules usually seen emanating from early cellularisation furrow canals.

(Lee and Harris, 2013)

Grp1^k08110/Grp1^SH0323 male and female adults are significantly smaller and show a drastic decrease in weight compared to control animals, although the body proportions of the mutant animals are not altered compared to wild type. Homozygous and Grp1^k08110/Grp1^SH0323 larvae are also significantly smaller than controls.

(Fuss et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

step^SH0323/step^k08110 has embryo | embryonic stage 5 phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

step^SH0323/step^k08110 has furrow canal phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

Enhancer of

Statement

Reference

step^SH0323/step[+] is an enhancer of embryo | embryonic stage 5 phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

step^SH0323/step[+] is an enhancer of furrow canal phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

step^SH0323/step[+] is an enhancer of embryo | embryonic stage 4 phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

NOT Suppressor of

Statement

Reference

step^SH0323/step^k08110 is a non-suppressor of phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Fuss et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Maternal heterozygosity for Rho1^72O almost completely suppresses the furrow canal expansion phenotype seen in step^SH0323/step^k08110 mutant embryos.

Maternal heterozygosity for step^SH0323 enhances the furrow loss phenotype seen in embryos derived from mothers expressing AP-2α^HMS00653 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Lee and Harris, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Grp1^SH0323

l(2)SH0323^SH0323

step^SH0323

(Lee and Harris, 2013, Fuss et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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