Expression of AP-2α^HMS00653 under the control of Scer\GAL4^ppk.PU (and Dicer-2, for efficient RNAi) results in mild but highly penetrant ddaC neuron dendrite pruning defects at 16h after puparium formation, as compared to controls.

Expression of AP-2α^HMS00653 under the control of Scer\GAL4^ey.PU induces a headless phenotype in pupae. Expression under the control of Scer\GAL4^elav-C155 or Scer\GAL4^Toll-6-D42 results in sluggish 3rd instar larvae which die. Expression under the control of Scer\GAL4^elav-C155 also leads to a significant increase in the number of boutons at larval muscle 6/7 NMJs, as compared to controls; electrophysiology recording show significant increases in mEJP frequency and amplitude, significant decreases in EJP amplitude and quantal content, and a significant decline in EJP amplitude upon high-frequency stimulation at 34[o]C conditions, even after a 4min rest period.

Under starvation conditions, the expression of AP-2α^HMS00653 under the control of Scer\GAL4^Cg.PA results in the third instar larval fat body showing an the accumulation and enlargement of early and late endosomes (identified as GFP-FYVE and GFP-Rab7 cytoplasmic puncta, respectively), an enlargement of lysosomes (identified as LAMP-GFP puncta) and their accumulation at the cell membrane, and an accumulation and enlargement of autophagosomes (identified as GFP-Atg8 cytoplasmic puncta and p62 protein cytoplasmic puncta), as compared to starved controls. However, similar defects in early and late endosomes, lysosomes and autophagosomes are also observed under normal diet.

Expression of AP-2α^HMS00653 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in melanisation of the fat body.

Expression of AP-2α^HMS00653 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in embryos with two distinct plasma membrane furrow defects. The first class of embryos exhibit various degrees of furrow loss, while the second class display abnormal plasma membrane expansions, specifically at furrow tips at both cellularisation and earlier syncytial divisions. This second class also lacks the endocytic tubules that usually emanate from the furrow canals.

AP-2α^HMS00653, Scer\GAL4^ppk.PU has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by prd1^M56/prd1[+]

AP-2α^HMS00653, Scer\GAL4^ppk.PU has abnormal size | pupal stage P5 phenotype, enhanceable by prd1^M56/prd1[+]

AP-2α^HMS00653, ND-42^ninaE.GD6220, Scer\GAL4^54C has abnormal neuroanatomy | adult stage phenotype

AP-2α^HMS00653, ND-42^ninaE.GD6220, Scer\GAL4^54C has abnormal neurophysiology | adult stage phenotype

AP-2α^HMS00653, Scer\GAL4^ppk.PU has dendritic tree | pupal stage P5 phenotype, enhanceable by prd1^M56/prd1[+]

AP-2α^HMS00653, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by prd1^M56/prd1[+]

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has embryo | embryonic stage 5 phenotype, enhanceable by AP-2sigma[+]/AP-2σ^KG02457

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has furrow canal phenotype, enhanceable by AP-2sigma[+]/AP-2σ^KG02457

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has embryo | embryonic stage 4 phenotype, enhanceable by AP-2sigma[+]/AP-2σ^KG02457

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has embryo | embryonic stage 5 phenotype, enhanceable by step^SH0323/step[+]

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has furrow canal phenotype, enhanceable by step^SH0323/step[+]

AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C} has embryo | embryonic stage 4 phenotype, enhanceable by step^SH0323/step[+]

AP-2α^HMS00653, Scer\GAL4^54C is a suppressor of lipid droplet | adult stage phenotype of ND-42^ninaE.GD6220, Scer\GAL4^54C

AP-2α^HMS00653, Scer\GAL4^54C is a suppressor of pigment cell | adult stage phenotype of ND-42^ninaE.GD6220, Scer\GAL4^54C

AP-2α^HMS00653, Scer\GAL4^ninaE.PU is a non-suppressor of lipid droplet | adult stage phenotype of ND-42^ninaE.GD6220, Scer\GAL4^ninaE.PU

AP-2α^HMS00653, Scer\GAL4^ninaE.PU is a non-suppressor of pigment cell | adult stage phenotype of ND-42^ninaE.GD6220, Scer\GAL4^ninaE.PU

AP-2α^HMS00653, ND-42^ninaE.GD6220, Scer\GAL4^54C has rhabdomere | adult stage phenotype

AP-2α^HMS00653, ND-42^ninaE.GD6220, Scer\GAL4^54C has retina | adult stage phenotype