FlyBase Allele Report: Dmel\fru[MC.UAS.cSa]

General Information

Symbol

Dmel\fru^MC.UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0144288

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

Carried in Construct

P{UAS-fru.MC.S}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Song et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-fru.MC.S}

Cytology

Description

UASt regulatory sequences drive expression of fru consisting of sequences encoding the 101 male specific amino terminus and the BTB domain derived from the fru male cDNA nos. 5-19 fused to the "C" 3' end.

(Song et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

fru

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^fru.16

(Billeter et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^sca-537.4, fru^w12/fru^sat15

(Song et al., 2002)

Phenotype Manifest In

larval longitudinal connective, with Scer\GAL4^sca-537.4, fru^w12/fru^sat15

(Song et al., 2002)

presumptive embryonic/larval central nervous system, with Scer\GAL4^sca-537.4, fru^w12/fru^sat15

(Song et al., 2002)

serotonergic neuron, with Scer\GAL4^fru.16

(Billeter et al., 2006)

Detailed Description

Statement

Reference

Approximately 32% of wild-type females expressing fru^{MB.Scer\UAS.cSa} under the control of Scer\GAL4^fru.16 develop on average two ectopic `male' serotonergic neurons, sending projections into the abdominal nerve trunk. Ectopic serotonergic terminals innervated the female internal reproductive organs, mostly the calyx of the ovaries but also, more rarely, the uterus and the base of the spermathecae. These serotonergic terminals are never present in virgin or fertilised wild-type females. The ectopic serotonergic neurons and their associated terminalia are analogous to those innervating the male reproductive organs.

(Billeter et al., 2006)

The Fas2-positive and BP102-positive axon tracts in the central nervous system are defective in virtually all fru^w12/fru^sat15 embryos which are expressing fru^{MC.Scer\UAS.cSa} under the control of Scer\GAL4^sca-537.4.

(Song et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

fru^MC.UAS.cSa/Scer\GAL4^fru.16 rescues fru³/fru^ΔC

(Billeter et al., 2006)

fru^MC.UAS.cSa/Scer\GAL4^fru.16 rescues fru³

(Billeter et al., 2006)

Fails to rescue

fru^MC.UAS.cSa/Scer\GAL4^fru.16 fails to rescue fru³

(Billeter et al., 2006)

Comments

Expression of fru^{MC.Scer\UAS.cSa}, under the control of Scer\GAL4^fru.16 in a fru³/fru³ background does not restore male fertility.

Expression of fru^{MC.Scer\UAS.cSa} under the control of Scer\GAL4^fru.16 in fru³ homozygotes and fru³/fru^ΔC transheterozygotes rescues the formation of the muscle of Lawrence in the male.

Extension of mating length beyond normal duration in fru^ΔC/fru³ males is rescued by targeted expression of fru^{MC.Scer\UAS.cSa} via Scer\GAL4^fru.16.

Expression of fru^{MC.Scer\UAS.cSa}, under the control of Scer\GAL4^fru.16 in a fru³ homozygote does not restore male fertility. However, expression of fru^{MC.Scer\UAS.cSa}, under the control of Scer\GAL4^fru.16 in a fru^ΔC/fru³ background, increases the percentage of fertile males from 33% to 78%.

During a 6 hour observation, fru^ΔC/fru³ males do not mate, whereas approximately 50% of fru^ΔC/fru³ males expressing fru^{MC.Scer\UAS.cSa} do. These males are slower to initiate mating, although the duration of copulation is similar to that of wild-type males. A small number of control flies (i.e. fru^{MC.Scer\UAS.cSa}/+ ; fru^ΔC/fru³) manage to mate within the same time as the 'rescued' males, probably due to 'leakage' of the transgene, although mating durations are still significantly longer than those of wild-type males.

(Billeter et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fru^{MC.Scer\UAS.cSa}

fru^MC.UAS.cSa

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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