FlyBase Allele Report: Dmel\CDase[UAS.cAa]

General Information

Symbol

Dmel\CDase^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Acharya

FlyBase ID

FBal0145166

Feature type

allele

Associated gene

Dmel\CDase

Associated Insertion(s)

Carried in Construct

P{UAS-CDase.A}

Also Known As

UAS-Ceramidase, UAS-CDase

Key Links

Transgenic product class

wild_type

(Acharya et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Acharya et al., 2003)

Mutagen

in vitro construct

(Acharya et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-CDase.A}

Cytology

Description

UAS regulatory sequences drive expression of CDase.

(Acharya et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

CDase

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates retinal disease

modeled by norpA^P41

(Acharya et al., 2003)

exacerbates primary autosomal dominant microcephaly 18

modeled by bchs⁵⁸

(Hebbar et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF does not effect photoreceptor integrity.

(Acharya et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of decreased cell number | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Suppressor of

Statement

Reference

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of abnormal neurophysiology phenotype of Cerk¹

(Dasgupta et al., 2009)

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of abnormal neurophysiology phenotype of Arr2³

(Acharya et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of larval RP2 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of larval DA1 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

CDase^UAS.cAa/Scer\GAL4^hs.PB is an enhancer of rhabdomere phenotype of ninaE¹⁷

(Acharya et al., 2004)

CDase^UAS.cAa/Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of ninaE¹⁷

(Acharya et al., 2004)

NOT Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is a non-enhancer of autophagosome | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^elav-C155/CDase^UAS.cAa is a non-enhancer of eye phenotype of cathD¹

(Kuronen et al., 2009)

Suppressor of

Statement

Reference

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of rhabdomere phenotype of Cerk¹

(Dasgupta et al., 2009)

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell phenotype of Cerk¹

(Dasgupta et al., 2009)

Scer\GAL4^unspecified/CDase^UAS.cAa is a suppressor of eye photoreceptor cell phenotype of Arr2³

(Acharya et al., 2008)

CDase^UAS.cAa is a suppressor of photoreceptor cell R2 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R5 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R6 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of photoreceptor cell R1 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of rhabdomere phenotype of Arr2³

(Acharya et al., 2003)

CDase^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of rhabdomere phenotype of norpA^P41

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of photoreceptor cell R3 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of photoreceptor cell R4 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of photoreceptor cell R5 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of photoreceptor cell R6 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R1 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R2 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R3 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

CDase^UAS.cAa is a suppressor of rhabdomere R4 phenotype of Scer\GAL4^GMR.PF, shi^1.UAS

(Acharya et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is a non-suppressor of autophagosome | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF rescues the lack of a light-induced electroretinogram response seen in Cerk¹ flies. The light-induced retinal degeneration that is seen in Cerk¹ flies is also rescued by expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF.

(Dasgupta et al., 2009)

The photoreceptor degeneration seen in Arr2³ mutants is suppressed by expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^unspecified in the fat body.

Expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^unspecified in the fat body enhances the turnover of involuting rhabdomeric elements in ninaE¹⁷ mutants.

(Acharya et al., 2008)

CDase^Scer\UAS.cAa (driven by Scer\GAL4^GMR.PF) enhances the rhabdomere phenotype seen in ninaE¹⁷ animals. Very few rhabdomeric elements remain at the apical surface of the photoreceptors in three day old animals of this genotype. Most of the rhabdomeric membranes are internalised and are in the process of being cleared. When CDase^Scer\UAS.cAa is driven by Scer\GAL4^hs.PB and expressed in ninaE¹⁷ flies (under heat shock) just after eclosion, most of the rhabdomere is cleared from the apical region, unlike the phenotype seen in ninaE¹⁷ animals alone.

(Acharya et al., 2004)

Expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF suppresses rhabdomere degeneration in Arr2³ flies and results in ommatidia that are similar to those of wild-type flies. Arr2³ flies expressing CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF have an electroretinogram with a large amplitude response, but the slow inactivation kinetics seen in Arr2³ flies are not rescued. The degeneration of photoreceptor cells seen in flies expressing shi^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 23^oC is suppressed by coexpression of CDase^Scer\UAS.cAa; rhabdomeres are largely intact, there are fewer vacuolated cells and the trapezoidal arrangement of the rhabdomeres is retained. Expression of CDase^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF suppresses the retinal degeneration seen in norpA³⁹ flies.

(Acharya et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CDase^Scer\UAS.cAa

CDase^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Acharya

(Acharya et al., 2003)

Secondary FlyBase IDs

References (7)

Report Sections

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