FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.
The spindle is asymmetric in telophase pI cells (6/8) of rapsunspecified homozygous pupae; however, in this case the spindle posterior arm is preferentially extended relative to the anterior spindle arm.
pinsunspecified is an enhancer of abnormal neuroanatomy phenotype of locoP283
pinsunspecified is an enhancer of astral microtubule & neuroblast | ectopic phenotype of locoP283
pinsunspecified is an enhancer of neuroblast phenotype of locoP283
pinsunspecified is a suppressor of spindle pole centrosome phenotype of GαiUAS.cCa, Scer\GAL4sca-P309
insc22, pinsunspecified has spindle pole centrosome & astral microtubule phenotype
In embryos derived from double germline clones of locoP283 and rapsunspecified, the majority of neuroblasts undergo symmetric divisions to generate two similar-sized daughter cells in stage 10 mutant embryos, in 60% of cases. The cleavage plane is placed near the middle of the two centrosomes and the spindle is positioned symmetrically with both centrosomes lying in close proximity to the cell cortex. Astral microtubules, which are normally associated only with the apical centrosome in wild-type neuroblasts, can emanate from both centrosomes in locoP283 and rapsunspecified double mutant neuroblasts.
In rapsunspecified insc22 double mutant neuroblasts, both centrosomes are associated with astral microtubules, with a cap structure forming over each centrosome from metaphase onwards. This phenotype is not observed in single mutants for these genes. Expression of G-iα65AScer\UAS.cCa, under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in a rapsunspecified background results in equal size division occurring in 62% of neuroblasts.