FlyBase Allele Report: Dmel\Smurf[15C]

General Information

Symbol

Dmel\Smurf^15C

Species

D. melanogaster

Name

FlyBase ID

FBal0147051

Feature type

allele

Associated gene

Dmel\Smurf

Associated Insertion(s)

H{}Smurf^15C

Carried in Construct

Also Known As

DSmurf^15C

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

hobo activity

Nature of the Allele

Allele class

Mutagen

hobo activity

(Podos et al., 2001)

Progenitor genotype

Associated Insertion(s)

H{}Smurf^15C

Cytology

Description

hobo insertion following codon 608 of lack.

(Podos et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,601,639..17,601,639 [+]

(Podos et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Podos et al., 2001)

lethal | rescuable maternal effect

(Podos et al., 2001)

viable

(Podos et al., 2001)

Phenotype Manifest In

amnioserosa | rescuable maternal effect

(Podos et al., 2001)

embryonic/first instar larval cuticle | rescuable maternal effect

(Podos et al., 2001)

embryonic head | rescuable maternal effect

(Podos et al., 2001)

embryonic hindgut | rescuable maternal effect

(Podos et al., 2001)

stomodeum | dorsal | rescuable maternal effect

(Podos et al., 2001)

Detailed Description

Statement

Reference

99% of lack^15C homozygous progeny of lack^15C homozygous mothers die as embryos. Most cuticles from these dead embryos have a hole in the dorsal posterior region extending from the spiracles to the posterior pole, or a more extensive dorsal hole (approximately 50%). These embryos also have variable head defects, and an excess of amnioserosa cells at stage 12 (336+/-47 (n=5) compared to 176+/-54 (n=3) for wild-type). From stage 14 onwards lack^15C homozygous progeny of lack^15C homozygous mothers display hindgut abnormalities: The hindgut is shorter and wider than in wild-type; bulges out of the dorsal surface of the embryo; and is splayed open, exposing the lumen. These embryos also display an abnormal variability in the number and shape of epithelial invaginations in the dorsal stomodeum.

(Podos et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Smurf^15C has lethal | rescuable maternal effect phenotype, suppressible by dpp^H46/dpp[+]

(Podos et al., 2001)

Enhancer of

Statement

Reference

Smurf[+]/Smurf^15C is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, wts^UAS.cYa

(Cao et al., 2014)

Smurf[+]/Smurf^15C is an enhancer of abnormal size phenotype of Scer\GAL4^Tub.PU, wts^UAS.cYa

(Cao et al., 2014)

Smurf[+]/Smurf^15C is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, yki^UAS.cHa

(Cao et al., 2014)

Smurf[+]/Smurf^15C is an enhancer of abnormal size phenotype of Scer\GAL4^Tub.PU, yki^UAS.cHa

(Cao et al., 2014)

Suppressor of

Statement

Reference

Smurf^15C/lack[+] is a suppressor of lethal | maternal effect phenotype of Med¹⁵

(Podos et al., 2001)

Smurf^15C is a suppressor of lethal | dominant phenotype of dpp^H46

(Podos et al., 2001)

NOT Suppressor of

Statement

Reference

Smurf^15C is a non-suppressor of lethal phenotype of dpp^H46

(Podos et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Smurf^15C has embryonic hindgut | rescuable maternal effect phenotype, suppressible by dpp^H46/dpp[+]

(Podos et al., 2001)

Enhancer of

Statement

Reference

Smurf[+]/Smurf^15C is an enhancer of eye phenotype of Scer\GAL4^Tub.PU, wts^UAS.cYa

(Cao et al., 2014)

Smurf[+]/Smurf^15C is an enhancer of eye phenotype of Scer\GAL4^Tub.PU, yki^UAS.cHa

(Cao et al., 2014)

Smurf^15C is an enhancer of larval dorsal hair phenotype of sog^Y506

(Podos et al., 2001)

Smurf^15C is an enhancer of embryonic/first instar larval cuticle phenotype of sog^Y506

(Podos et al., 2001)

Smurf^15C is an enhancer of ventral denticle belt phenotype of sog^Y506

(Podos et al., 2001)

NOT Suppressor of

Statement

Reference

Smurf^15C is a non-suppressor of cuticle phenotype of dpp^H46

(Podos et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Smurf^15C dominantly enhances the small and rough eye phenotype resulting from the expression of Scer\GAL4^tub.PU>wts^Scer\UAS.cYa.

Smurf^15C dominantly enhances the enlarged eyes caused by overexpressing Scer\GAL4^tub.PU>yki^Scer\UAS.cHa.

(Cao et al., 2014)

Homozygous lack^15C has no effect on the increased number of spectrosome-containing GSC-like cells seen in fu⁴¹ mutant germaria.

Expression of tkv^{ca.bam.T:Avic\GFP} enhances the delay in cystoblast differentiation seen in lack^15C mutant germaria. Nealy 38% of ovarioles are composed of a tumorous germarium, and 62% of the ovarioles contains tumorous germaria that are attached to one or several egg chambers.

(Xia et al., 2010)

In piwi^unspecified lack^15C double mutant ovaries, most germaria contain supernumerary germline stem cells (GSCs) and a continuous supply of egg chambers, resembling the lack^15C single mutant phenotype.

(Chen and McKearin, 2005)

Embryonic lethality due to maternal and zygotic homozygosity for lack^15C is partially suppressed (83% survival to adulthood) by heterozygosity for dpp^H46. The embryonic midgut phenotype of maternal and zygotic lack^15C/lack^15C embryos is fully suppressed by dpp^H46/+ and sog^Y506/sog^Y506 and partially suppressed by zen⁷/zen⁷ (in the latter embryos, the hindgut frequently fails to form its normal hook-like morphology). Maternal and zygotic homozygosity for lack^15C partially suppresses the dominant lethality of dpp^H46 (83% survival to adulthood), but has no effect on the lethality or cuticle phenotypes due to homozygosity for dpp^H46. The weak dorsalisation phenotype exhibited by cuticles from sog^Y506 hemizygous embryos is enhanced to full dorsalisation (transformation of all ventral denticle belts into dorsal hairs) by maternal and zygotic homozygosity for lack^15C.

(Podos et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DSmurf^15C

(Podos et al., 2001)

Dsmurf 15c

(Chen and McKearin, 2005)

Smurf^15C

(Lin et al., 2017, Chang et al., 2013, Casanueva and Ferguson, 2004)

dSmurf^15C

(Cao et al., 2014)

lack^15C

smurf^15C

(Sullivan and Bashaw, 2025, Xia et al., 2010)

smurf^15c

(Huang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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