FlyBase Allele Report: Dmel\Med[15]

General Information

Symbol

Dmel\Med¹⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0086802

Feature type

allele

Associated gene

Dmel\Med

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hudson et al., 1998)

Progenitor genotype

Cytology

Description

Amino acid replacement: A679V. Amino acid replacement: T714K.

(Hudson et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,614,699..31,614,699 [+]

Nucleotide change:

C31614699T

Amino acid change:

A705V | Med-PA; A631V | Med-PB; A705V | Med-PC

Reported amino acid change:

A679V

Comment:

One of two amino acid changes reported in Med¹⁵ mutant.

(Hudson et al., 1998)

point_mutation

3R:31,614,804..31,614,804 [+]

Nucleotide change:

C31614804A

Amino acid change:

T740K | Med-PA; T666K | Med-PB; T740K | Med-PC

Reported amino acid change:

T714K

Comment:

Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Hudson et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates muscular dystrophy

modeled by Scgδ⁸⁴⁰

(Goldstein et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult thorax

(Hudson et al., 1998)

embryonic/first instar larval cuticle | maternal effect

(Hudson et al., 1998)

embryonic epidermis

(Podos and Ferguson, 1998)

femur

(Hudson et al., 1998)

posterior crossvein

(Hudson et al., 1998)

pretarsus

(Hudson et al., 1998)

tarsal segment 4

(Hudson et al., 1998)

tibia

(Hudson et al., 1998)

wing vein L4

(Hudson et al., 1998)

Detailed Description

Statement

Reference

Homozygous adults do not have any obvious phenotypic abnormalities. Med¹¹/Med¹⁵ flies have a large medial cleft in the notum. Legs show shortening of the femur and tibia and loss of the fourth and fifth tarsal segments. Med¹³/Med¹⁵ flies lack the posterior crossvein and have a large discontinuity in wing vein L4. Embryos derived from homozygous females mated to wild-type males die with a partially ventralised phenotype.

(Hudson et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Med¹⁵ has lethal | maternal effect phenotype, suppressible by Smurf^15C/lack[+]

(Podos et al., 2001)

Med¹⁵ has lethal | maternal effect phenotype, suppressible by Smurf^11R/lack[+]

(Podos et al., 2001)

Enhancer of

Statement

Reference

Med[+]/Med¹⁵ is an enhancer of lethal | recessive | partially phenotype of sog^P129D

(Decotto and Ferguson, 2001)

Suppressor of

Statement

Reference

Med[+]/Med¹⁵ is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scgδ⁸⁴⁰

(Goldstein et al., 2011)

Other

Statement

Reference

Med[+]/Med¹⁵, dpp^e87 has lethal | maternal effect phenotype

(Quijano et al., 2016)

Med[+]/Med¹⁵, dpp^hr27 has lethal | maternal effect phenotype

(Quijano et al., 2016)

Med[+]/Med¹⁵, dpp^hr4 has partially lethal - majority die phenotype

(Quijano et al., 2016)

Med[+]/Med¹⁵, dpp^hr56 has lethal | maternal effect | maternal effect phenotype

(Quijano et al., 2016)

Med¹⁵, dpp^hr4/dpp[+] has lethal | maternal effect phenotype

(Stinchfield et al., 2012)

Med¹⁵, zen¹ has lethal | recessive | embryonic stage phenotype

(Hudson et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

Med[+]/Med¹⁵ is an enhancer of phenotype of dpp^hr4

(Hudson et al., 1998)

Suppressor of

Statement

Reference

Med[+]/Med¹⁵ is a suppressor of embryonic/first instar larval cuticle phenotype of sog^Y506

(Stinchfield et al., 2012)

Med[+]/Med¹⁵ is a suppressor of denticle phenotype of sog^Y506

(Stinchfield et al., 2012)

Other

Statement

Reference

Med[+]/Med¹⁵, dpp^hr4 has embryo | maternal effect phenotype

(Quijano et al., 2016)

Med¹⁵, zen¹ has embryonic/first instar larval cuticle phenotype

(Hudson et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The progeny from Med¹⁵/+ mothers and dpp^hr4/+ fathers present embryo ventralization in most cases and exhibit near lethality, as compared to controls; the progeny from Med¹⁵/+ mothers and either dpp^hr27/+, dpp^hr56/+ or dpp^e87/+ fathers exhibit lethality, as compared to controls.

(Quijano et al., 2016)

Animals derived from homozygous Med¹⁵ females mated to dpp^hr4/+ males show strong lethality (less than 5% of the expected number survive).

Med¹⁵/+ partially suppresses the cuticle phenotype of sog^Y506 hemizygous embryos. The embryos have partially restored denticles, a normal body shape and misplaced, misshapen Filzkorper.

(Stinchfield et al., 2012)

The severe loss of climbing ability in a negative geotaxis assay which is seen in Scgδ⁸⁴⁰ mutants is suppressed if the flies are also carrying Med¹⁵/+.

(Goldstein et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Med⁴

(Hudson et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Med¹⁵

(Quijano et al., 2016, Stinchfield et al., 2012)

Medea¹⁵

(Goldstein et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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