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General Information
Symbol
Dmel\Dscam133
Species
D. melanogaster
Name
FlyBase ID
FBal0148063
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Dscam33
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

anterior scutellar bristle & mechanosensory neuron | somatic clone

posterior dorsocentral bristle & mechanosensory neuron | somatic clone

posterior scutellar bristle & mechanosensory neuron | somatic clone

Detailed Description
Statement
Reference

Dscam33 posterior Dorsocentral (pDc), anterior Scutellar (aSc) and posterior Scutellar (pSc) neuron clones enter the thoracic ganglion at the correct position but are unable to correctly target axonal branches and fail to elaborate any arbors. These neurons are able to form axonal branches but these remain clustered in a small bolus around a presumptive decision point. This phenotype is 100% penetrant for all tested neuron clones.

The overall innervation pattern of Dscam33 mutant antennal olfactory receptor neurons through the antennal nerve into the antennal lobe is largely normal. Dscam33 mutant maxillary palp olfactory receptor neurons that project into the central nervous system along the labial nerve frequently terminate not within the antennal lobe but in regions surrounding it and the suboesophageal ganglion. Or47a-expressing olfactory receptor neurons (ORNs) that are mutant for Dscam33 show marked mis-targeting of axons in the antennal lobe, often failing to project to the normal target (glomerulus DM3) and instead innervating abnormal locations on the ipsilateral side. less frequently, targeting to ectopic or cognate sites in the contralateral lobe is seen. P{GAL4}GH298-expressing ORNs that are mutant for Dscam33 often fail to project to their normal target (the V glomerulus), innervating abnormal locations on the ipsilateral side. Single Dscam33 mutant Or47a-expressing ORNs exit the antennal nerve and often terminate in abnormal locations between glomeruli. Mutant terminals that do reach the appropriate glomerulus fail to elaborate the network of thin arbors characteristic of wild-type terminals. Or22a-expressing and Or23a-expressing Dscam33 mutant ORNs target normally to the correct glomerulus, although they show a marked reduction in branches to the contralateral antennal lobe. Or47b-expressing Dscam33 mutant ORNs show mistargeting to more dorsal regions of the antennal lobe than the normal target (the VA1 l/m glomerulus) and also targeting defects in the contralateral lobe. Or46a-expressing and Or59c-expressing Dscam33 mutant ORNs frequently terminate upon entering the ventral central nervous system before reaching the antennal lobe, either immediately prior to entering the suboesophageal ganglion or upon exiting it, just ventral to the antennal lobe. Or71a-expressing Dscam33 mutant ORNs frequently terminate upon entering the ventral central nervous system before reaching the antennal lobe, either immediately prior to entering the suboesophageal ganglion or upon exiting it, just ventral to the antennal lobe. The mutant ORNs form a structure with the appearance of a glomerulus where they terminate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

There is no evidence for a genetic interaction between tutl23 and Dscam33 when assayed by studying dendrite self-avoidance in class IV da neurons.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of transgenes that encode different isoforms of Dscam, under the control of Scer\GAL4elav.PLu, partially rescue the axonal targeting phenotype of Dscam33 mechanosensory clones. Both the Dscam1.34.30.2.Scer\UAS and Dscam7.6.19.2.Scer\UAS transgenes rescue primary axon extension posteriorly. Occasionally, Dscam1.30.30.2.A.Scer\UAS rescues the proximal, anterior-extending secondary axonal branch and a distal ipsilateral branch.

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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (5)