FlyBase Allele Report: Dmel\14-3-3ε[ex4]

General Information

Symbol

Dmel\14-3-3ε^ex4

Species

D. melanogaster

Name

FlyBase ID

FBal0148514

Feature type

allele

Associated gene

Dmel\14-3-3ε

Associated Insertion(s)

Carried in Construct

Also Known As

D14-3-3ε^ex4

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Acevedo et al., 2007, Chen et al., 2003)

Progenitor genotype

14-3-3ε^j2B10

(Acevedo et al., 2007, Chen et al., 2003)

Cytology

Description

Small deletion removing the first exon and part of the first intron.

(Acevedo et al., 2007)

Imprecise mobilisation of the P{lacW}14-3-3ε^j2B10 element has created this allele.

(Chen et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size (with 14-3-3ε^j2B10)

(Nielsen et al., 2008)

female fertile (with 14-3-3ε^ex5)

(Tsigkari et al., 2012)

female sterile

(Tsigkari et al., 2012)

female sterile (with 14-3-3ε^j2B10)

(Tsigkari et al., 2012)

female sterile (with Df(3R)14-3-3ε^ex24)

(Tsigkari et al., 2012)

male fertile (with 14-3-3ε^ex5)

(Tsigkari et al., 2012)

male sterile

(Tsigkari et al., 2012)

male sterile (with 14-3-3ε^j2B10)

(Tsigkari et al., 2012)

male sterile (with Df(3R)14-3-3ε^ex24)

(Tsigkari et al., 2012)

partially lethal (with Df(3R)14-3-3ε^ex24)

(Acevedo et al., 2007)

partially lethal - majority die (with 14-3-3ε^j2B10)

(Acevedo et al., 2007)

partially lethal - majority die | recessive

(Acevedo et al., 2007)

some die during embryonic stage | recessive

(Acevedo et al., 2007)

viable (with 14-3-3ε^ex5)

(Acevedo et al., 2007)

visible (with 14-3-3ε^j2B10)

(Acevedo et al., 2007)

visible | recessive

(Acevedo et al., 2007)

Phenotype Manifest In

anterior crossvein

(Acevedo et al., 2007)

anterior crossvein (with 14-3-3ε^j2B10)

(Acevedo et al., 2007)

posterior crossvein

(Acevedo et al., 2007)

posterior crossvein (with 14-3-3ε^j2B10)

(Acevedo et al., 2007)

primordial germ cell

(Tsigkari et al., 2012)

Detailed Description

Statement

Reference

Homozygous females lay very few eggs and heterozygous females lay significantly fewer eggs than wild type.

Pole cells appears dispersed all over the embryo in heterozygous and homozygous 16-18 hours embryos with the number of pole cells per gonad being reduced compared to wild type in homozygous embryos at stages 8 and 11-12.

The somatic cells of the gonad are present and appear similar in number to wild type in heterozygous and homozygous embryos.

(Tsigkari et al., 2012)

No retinal phenotypes are seen in 14-3-3ε^ex4 mutant flies.

(Branco et al., 2008)

Homozygotes show 42% viability. 14-3-3ε^ex4/14-3-3ε^j2B10 animals show 71% viability.

Homozygous embryos do not show gross morphological defects.

Homozygous adults have smaller wings than control flies. 42.9% of homozygotes have defects in the anterior crossvein, while 80.9% have defects in the posterior crossvein.

26.7% of 14-3-3ε^ex4/14-3-3ε^j2B10 adults have defects in the anterior crossvein, while 82.4% have defects in the posterior crossvein.

(Acevedo et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

14-3-3ε^ex4 has partially lethal - majority die | recessive phenotype, suppressible | heat sensitive by 14-3-3ζ^LII.2.hs

(Acevedo et al., 2007)

14-3-3ε^ex4 has visible phenotype, suppressible | partially by 14-3-3ζ^LI.15.hs

(Acevedo et al., 2007)

14-3-3ε^ex4 has partially lethal - majority die | recessive phenotype, suppressible | heat sensitive | partially by 14-3-3ζ^LI.15.hs

(Acevedo et al., 2007)

14-3-3ε^ex4 has partially lethal - majority die | recessive phenotype, suppressible | partially by Scer\GAL4^elav-C155/14-3-3ζ^I.UAS

(Acevedo et al., 2007)

14-3-3ε^ex4 has partially lethal - majority die | recessive phenotype, suppressible by Scer\GAL4^elav-C155/14-3-3ζ^II.UAS

(Acevedo et al., 2007)

NOT suppressed by

Statement

Reference

14-3-3ε^ex4 has visible phenotype, non-suppressible by 14-3-3ζ^LII.2.hs

(Acevedo et al., 2007)

Enhancer of

Statement

Reference

14-3-3ε^ex4 is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Shirasaki et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

14-3-3ε^ex4 has posterior crossvein phenotype, suppressible | partially by 14-3-3ζ^LI.15.hs

(Acevedo et al., 2007)

14-3-3ε^ex4 has anterior crossvein phenotype, suppressible by 14-3-3ζ^LI.15.hs

(Acevedo et al., 2007)

NOT suppressed by

Statement

Reference

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by 14-3-3ζ^I.UAS/Scer\GAL4^αTub84B.PL

(Tsigkari et al., 2012)

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by Scer\GAL4^Act5C.PI/14-3-3ζ^I.UAS

(Tsigkari et al., 2012)

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/14-3-3ζ^I.UAS

(Tsigkari et al., 2012)

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by 14-3-3ζ^II.UAS/Scer\GAL4^αTub84B.PL

(Tsigkari et al., 2012)

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by Scer\GAL4^Act5C.PI/14-3-3ζ^II.UAS

(Tsigkari et al., 2012)

14-3-3ε^ex4 has primordial germ cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/14-3-3ζ^II.UAS

(Tsigkari et al., 2012)

14-3-3ε^ex4 has anterior crossvein phenotype, non-suppressible by 14-3-3ζ^LII.2.hs

(Acevedo et al., 2007)

14-3-3ε^ex4 has posterior crossvein phenotype, non-suppressible by 14-3-3ζ^LII.2.hs

(Acevedo et al., 2007)

Suppressor of

Statement

Reference

14-3-3ε^ex4/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

14-3-3ε^ex4/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of 14-3-3ζ^I.Scer\UAS under the control of either Scer\GAL4^αTub84B.PL, Scer\GAL4^Act5C.PI or Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3ε^ex4 embryos.

Expression of 14-3-3ζ^II.Scer\UAS under the control of either Scer\GAL4^αTub84B.PL, Scer\GAL4^Act5C.PI or Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3ε^ex4 embryos.

(Tsigkari et al., 2012)

Xenogenetic Interactions

Statement

Reference

One copy of 14-3-3ε^ex4 suppresses the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of 14-3-3ε^ex4 suppresses the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Rescued by

14-3-3ε^ex4 is rescued by 14-3-3ε^UAS.Tag:MYC/Scer\GAL4^Tub.PU

(Acevedo et al., 2007)

14-3-3ε^ex4 is rescued by Scer\GAL4^elav-C155/14-3-3ε^UAS.Tag:MYC

(Acevedo et al., 2007)

14-3-3ε^ex4 is rescued by 14-3-3ε^hs.PA

(Acevedo et al., 2007)

Partially rescued by

14-3-3ε^ex4 is partially rescued by 14-3-3ε^UAS.cCa/Scer\GAL4^αTub84B.PL

(Tsigkari et al., 2012)

Not rescued by

14-3-3ε^ex4 is not rescued by 14-3-3ε^UAS.cCa/Scer\GAL4^Act5C.PI

(Tsigkari et al., 2012)

Comments

The reduction in the number of pole cells per gonad seen in 14-3-3ε^ex4 embryos is almost completely rescued by expression of 14-3-3ε^Scer\UAS.cCa under the control of Scer\GAL4^αTub84B.PL but not under the control of Scer\GAL4^Act5C.PI.

(Tsigkari et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

14-3-3ε^Exc4

(Nielsen et al., 2008)

14-3-3ε^ex4

(Shirasaki et al., 2012, Branco et al., 2008)

14-3-3ε^exc4

(Nielsen et al., 2008)

D14-3-3ε^ex4

(Lalle et al., 2013, Tsigkari et al., 2012, Messaritou et al., 2010, Acevedo et al., 2007)

eps^ex4

(Tsigkari et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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