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General Information
Symbol
Dmel\14-3-3εex4
Species
D. melanogaster
Name
FlyBase ID
FBal0148514
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
D14-3-3εex4
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Small deletion removing the first exon and part of the first intron.

Imprecise mobilisation of the P{lacW}14-3-3εj2B10 element has created this allele.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous females lay very few eggs and heterozygous females lay significantly fewer eggs than wild type.

Pole cells appears dispersed all over the embryo in heterozygous and homozygous 16-18 hours embryos with the number of pole cells per gonad being reduced compared to wild type in homozygous embryos at stages 8 and 11-12.

The somatic cells of the gonad are present and appear similar in number to wild type in heterozygous and homozygous embryos.

No retinal phenotypes are seen in 14-3-3εex4 mutant flies.

Homozygotes show 42% viability. 14-3-3εex4/14-3-3εj2B10 animals show 71% viability.

Homozygous embryos do not show gross morphological defects.

Homozygous adults have smaller wings than control flies. 42.9% of homozygotes have defects in the anterior crossvein, while 80.9% have defects in the posterior crossvein.

26.7% of 14-3-3εex4/14-3-3εj2B10 adults have defects in the anterior crossvein, while 82.4% have defects in the posterior crossvein.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

14-3-3εex4/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\HTT128Q.1-336.UAS, Scer\GAL4GMR.PU

14-3-3εex4/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\ATXN182Q.UAS, Scer\GAL4GMR.PU

Additional Comments
Genetic Interactions
Statement
Reference

Expression of 14-3-3ζI.Scer\UAS under the control of either Scer\GAL4αTub84B.PL, Scer\GAL4Act5C.PI or Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3εex4 embryos.

Expression of 14-3-3ζII.Scer\UAS under the control of either Scer\GAL4αTub84B.PL, Scer\GAL4Act5C.PI or Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3εex4 embryos.

Xenogenetic Interactions
Statement
Reference

One copy of 14-3-3εex4 suppresses the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

One copy of 14-3-3εex4 suppresses the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Partially rescued by
Comments

The reduction in the number of pole cells per gonad seen in 14-3-3εex4 embryos is almost completely rescued by expression of 14-3-3εScer\UAS.cCa under the control of Scer\GAL4αTub84B.PL but not under the control of Scer\GAL4Act5C.PI.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)